Abstract
We isolated an Arabidopsis lesion initiation 2 (lin2) mutant, which develops lesion formation on leaves and siliques in a developmentally regulated and light-dependent manner. The phenotype of the lin2 plants resulted from a single nuclear recessive mutation, and LIN2 was isolated by a T-DNA tagging approach. LIN2 encodes coproporphyrinogen III oxidase, a key enzyme in the biosynthetic pathway of chlorophyll and heme, a tetrapyrrole pathway, in Arabidopsis. The lin2 plants express cytological and molecular markers associated with the defense responses, usually activated by pathogen infection. These results demonstrate that a porphyrin pathway impairment is responsible for the lesion initiation phenotype, which leads to the activation of defense responses, in Arabidopsis. Lesion formation was not suppressed, and was even enhanced when accumulation of salicylic acid (SA) was prevented in lin2 plants by the expression of an SA-degrading salicylate hydroxylase (nahG) gene. This suggests that the lesion formation triggered in lin2 plants is determined prior to or independently of the accumulation of SA but that the accumulation is required to limit the spread of lesions in lin2 plants.
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Claims 10
Claim · model-written summary
The lin2 mutant develops lesion formation on leaves and siliques in a developmentally regulated and light-dependent manner.
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- lin2e1
- Organ / stage
- leaves and siliquese1
- Outcome
- lesion formatione1
- Qualifier
- developmentally regulated and light-dependent mannere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We isolated an Arabidopsis lesion initiation 2 (lin2) mutant, which develops lesion formation on leaves and siliques in a developmentally regulated and light-dependent manner”
Claim · model-written summary
The lin2 phenotype results from a single nuclear recessive mutation.
- Taxon
- ArabidopsisArabidopsist0
- Material
- lin2 plantse2
- Outcome
- phenotypee2
- Qualifier
- resulted from a single nuclear recessive mutatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“causes lesion formation in Arabidopsis”
e2“The phenotype of the lin2 plants resulted from a single nuclear recessive mutation”
Claim · model-written summary
LIN2 encodes coproporphyrinogen III oxidase, a key enzyme of chlorophyll and heme biosynthesis in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- LIN2e3
- Outcome
- LIN2 encodes coproporphyrinogen III oxidasee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“causes lesion formation in Arabidopsis”
e3“LIN2 encodes coproporphyrinogen III oxidase, a key enzyme in the biosynthetic pathway of chlorophyll and heme”
Claim · model-written summary
The lin2 plants express cytological and molecular markers associated with defense responses.
- Taxon
- ArabidopsisArabidopsist0
- Material
- lin2 plantse4
- Outcome
- cytological and molecular markerse4
- Qualifier
- associated with the defense responsese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“causes lesion formation in Arabidopsis”
e4“The lin2 plants express cytological and molecular markers associated with the defense responses, usually activated by pathogen infection”
Claim · model-written summary
A porphyrin pathway impairment is responsible for the lesion initiation phenotype in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise5
- Outcome
- lesion initiation phenotypee5
- Qualifier
- porphyrin pathway impairmente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“These results demonstrate that a porphyrin pathway impairment is responsible for the lesion initiation phenotype, which leads to the activation of defense responses, in Arabidopsis”
Claim · model-written summary
The porphyrin pathway impairment leads to activation of defense responses in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise5
- Outcome
- activation of defense responsese5
- Qualifier
- porphyrin pathway impairmente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“These results demonstrate that a porphyrin pathway impairment is responsible for the lesion initiation phenotype, which leads to the activation of defense responses, in Arabidopsis”
Claim · model-written summary
Expression of nahG did not suppress lesion formation in lin2 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- lin2 plantse6
- Treatment
- expression of an SA-degrading salicylate hydroxylase (nahG) genee6
- Gene
- nahGe6
- Compound
- salicylic acide6
- Outcome
- Lesion formatione6
- Direction
- not suppressede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“causes lesion formation in Arabidopsis”
e6“Lesion formation was not suppressed, and was even enhanced when accumulation of salicylic acid (SA) was prevented in lin2 plants by the expression of an SA-degrading salicylate hydroxylase (nahG) gene”
Claim · model-written summary
Expression of nahG enhanced lesion formation in lin2 plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- lin2 plantse6
- Treatment
- expression of an SA-degrading salicylate hydroxylase (nahG) genee6
- Gene
- nahGe6
- Compound
- salicylic acide6
- Outcome
- Lesion formatione6
- Direction
- enhancede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“causes lesion formation in Arabidopsis”
e6“Lesion formation was not suppressed, and was even enhanced when accumulation of salicylic acid (SA) was prevented in lin2 plants by the expression of an SA-degrading salicylate hydroxylase (nahG) gene”
Claim · model-written summary
Accumulation of SA is required to limit the spread of lesions in lin2 plants.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- SAe7
- Outcome
- spread of lesionse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“causes lesion formation in Arabidopsis”
e7“This suggests that the lesion formation triggered in lin2 plants is determined prior to or independently of the accumulation of SA but that the accumulation is required to limit the spread of lesions in lin2 plants”
Claim · model-written summary
Lesion formation in lin2 plants is determined prior to or independently of SA accumulation.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- SAe7
- Outcome
- lesion formatione7
- Qualifier
- determined prior to or independently of the accumulation of SAe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“causes lesion formation in Arabidopsis”
e7“This suggests that the lesion formation triggered in lin2 plants is determined prior to or independently of the accumulation of SA but that the accumulation is required to limit the spread of lesions in lin2 plants”
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See all 55 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.