Abstract
During fall 1998, volunteer watermelons (Citrullus lunatus L. (Thunb.) Matsum. & Nakai) showing leaf curl, crumpling, and yellowing symptoms were found in a commercial honeydew melon (Cucumis melo L. subsp. melo Inodorus group) field in the Imperial Valley of California. The plants were infected with a begomovirus (family Geminiviridae, genus Begomovirus) based on (i) a positive response in squash blots probed with a general begomovirus DNA probe (1) and (ii) amplification of DNA-A (≈1.2 kb) and DNA-B (≈1.4 kb) fragments by polymerase chain reaction (PCR) with degenerate DNA-A (PAL1v1978/PAR1c496) and DNA-B (PBL1v2040/PBR1c970) primers, respectively (3). The DNA-A and -B fragments were cloned and sequenced (GenBank accession nos. AF224760 [DNA-A] and AF224761 [DNA-B]). The DNA-A and -B fragments had a nearly identical (99.5%) common region (CR) of 186 (DNA-A) and 187 (DNA-B) nucleotides, indicating they were from the same begomovirus. Database searches conducted with these sequences revealed no high degree of sequence identity (i.e., >90%) with other begomoviruses, including Squash leaf curl virus (SqLCV [2]) from southern California. The partial AC1 sequence (669 nt) was most identical to Tomato severe leaf curl virus (ToSLCV) from Guatemala (83%) and SqLCV (81%), the partial AV1 sequence (135 nt) was most identical to Tomato golden mosaic virus from Brazil (84%) and SqLCV (81%), and the CR was most identical to Squash yellow mottle virus from Costa Rica (81%), ToSLCV (81%), and SqLCV (77%). The partial BV1 sequence (465 nt) was most identical to Bean calico mosaic virus and SqLCV (72%), and the partial BC1 sequence (158 nt) was most identical to SqLCV (75%). Watermelon seedlings bombarded with a DNA extract from infected watermelon volunteers developed crumpling and distortion symptoms, whereas seedlings bombarded with gold particles alone developed no symptoms. Geminivirus infection in symptomatic seedlings was confirmed by PCR. These results suggest a new begomovirus caused the disease symptoms in the watermelon volunteers. Leaf crumpling and curling symptoms were not observed in spring melons in the Imperial Valley in 1999, but on 2 July and 17 August 1999, cantaloupe (C. melo L. subsp. melo Cantalupensis group), muskmelon (C. melo L. subsp. melo Cantalupensis group), and watermelon plants with leaf crumpling and yellowing were found. These plants were infected with the new begomovirus based on sequence analysis of PCR-amplified DNA-A fragments (97 to 98% identity for CR and partial AC1 sequence). A survey of fall melons, conducted 23 to 24 September 1999, revealed widespread symptoms of leaf curl and crumpling on new growth of muskmelon plants in all seven commercial fields examined (estimated incidence 25 to 50%) and on watermelon volunteers. No such symptoms were observed on leaves of honeydew melons. Symptomatic muskmelon and watermelon leaves, collected from eight locations throughout the Imperial Valley, were infected with the new begomovirus based on sequence analysis of PCR-amplified DNA-A fragments. Thus, a new begomovirus has emerged in the Imperial Valley; the name Cucurbit leaf crumple virus (CuLCrV) is proposed. References: (1) R. L. Gilbertson et al. Plant Dis. 75: 336, 1991. (2) S. G. Lazarowitz and I. B. Lazdins. Virology 180:58, 1991. (3) M. R. Rojas et al. Plant Dis. 77:340, 1993.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 15
Claim · model-written summary
Watermelon seedlings bombarded with a DNA extract from infected watermelon volunteers developed crumpling and distortion symptoms, whereas seedlings bombarded with gold particles alone developed no symptoms.
- Taxon
- Watermelon
Citrullus lanatuse0 - Treatment
- DNA extract from infected watermelon volunteerse0
- Outcome
- crumpling and distortion symptomse0
- Comparator
- gold particles alonee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Watermelon seedlings bombarded with a DNA extract from infected watermelon volunteers developed crumpling and distortion symptoms, whereas seedlings bombarded with gold particles alone developed no symptoms”
Claim · model-written summary
Geminivirus infection was confirmed by PCR in symptomatic seedlings.
- Taxon
- Cucurbitst0
- Interactor
- GeminivirusGeminiviridaee0pathogen
- Outcome
- Geminivirus infectione0
- Qualifier
- in symptomatic seedlingse0
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“Geminivirus infection in symptomatic seedlings was confirmed by PCR”
Claim · model-written summary
The common regions of the DNA-A and DNA-B fragments were nearly identical, 99.5%.
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“The DNA-A and -B fragments had a nearly identical (99.5%) common region (CR) of 186 (DNA-A) and 187 (DNA-B) nucleotides”
Claim · model-written summary
The new begomovirus showed no high sequence identity (above 90%) with other begomoviruses.
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“Database searches conducted with these sequences revealed no high degree of sequence identity (i.e., >90%) with other begomoviruses”
Claim · model-written summary
The partial AC1 sequence was most identical to Tomato severe leaf curl virus (83%).
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“The partial AC1 sequence (669 nt) was most identical to Tomato severe leaf curl virus (ToSLCV) from Guatemala (83%)”
Claim · model-written summary
The partial AC1 sequence was most identical to SqLCV (81%).
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“The partial AC1 sequence (669 nt) was most identical to Tomato severe leaf curl virus (ToSLCV) from Guatemala (83%) and SqLCV (81%)”
Claim · model-written summary
The partial AV1 sequence was most identical to Tomato golden mosaic virus (84%).
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“The partial AV1 sequence (135 nt) was most identical to Tomato golden mosaic virus from Brazil (84%) and SqLCV (81%)”
Claim · model-written summary
The common region was most identical to Squash yellow mottle virus (81%).
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“the CR was most identical to Squash yellow mottle virus from Costa Rica (81%), ToSLCV (81%), and SqLCV (77%)”
Claim · model-written summary
The partial BV1 sequence was most identical to Bean calico mosaic virus and SqLCV (72%).
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“The partial BV1 sequence (465 nt) was most identical to Bean calico mosaic virus and SqLCV (72%)”
Claim · model-written summary
The partial BC1 sequence was most identical to SqLCV (75%).
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“the partial BC1 sequence (158 nt) was most identical to SqLCV (75%)”
Claim · model-written summary
Cantaloupe, muskmelon and watermelon plants with leaf crumpling and yellowing were found in July and August 1999.
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“on 2 July and 17 August 1999, cantaloupe (C. melo L. subsp. melo Cantalupensis group), muskmelon (C. melo L. subsp. melo Cantalupensis group), and watermelon plants with leaf crumpling and yellowing were found”
Claim · model-written summary
Widespread leaf curl and crumpling occurred on muskmelon in all seven commercial fields surveyed in fall 1999, at an estimated incidence of 25 to 50%.
- Taxon
- muskmelon
.jpg?width=60)
Cucumis meloe0 - Outcome
- leaf curl and crumplinge0
- Value
- 25 to 50%e0
- Qualifier
- widespreade0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“revealed widespread symptoms of leaf curl and crumpling on new growth of muskmelon plants in all seven commercial fields examined (estimated incidence 25 to 50%)”
Claim · model-written summary
No such symptoms were observed on honeydew melon leaves.
- Taxon
- honeydew melons
Cucumis melo var. inodoruse0 - Outcome
- symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No such symptoms were observed on leaves of honeydew melons”
Claim · model-written summary
Symptomatic muskmelon and watermelon leaves from eight locations in the Imperial Valley were infected with the new begomovirus.
- Taxon
- muskmelon and watermelonCitrullus lanatusCucumis meloe0
- Interactor
- new begomoviruse0pathogen
- Outcome
- infected with the new begomoviruse0
- Setting
- eight locations throughout the Imperial Valleye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Symptomatic muskmelon and watermelon leaves, collected from eight locations throughout the Imperial Valley, were infected with the new begomovirus based on sequence analysis of PCR-amplified DNA-A fragments”
Claim · model-written summary
A new begomovirus, named Cucurbit leaf crumple virus, has emerged in the Imperial Valley.
- Taxon
- Cucurbitst0
- Interactor
- Cucurbit leaf crumple virusCucurbit leaf crumple viruse0pathogen
- Outcome
- a new begomovirus has emerged in the Imperial Valleye0
Verbatim
t0“Leaf Curl and Crumpling in Cucurbits”
e0“Thus, a new begomovirus has emerged in the Imperial Valley; the name Cucurbit leaf crumple virus (CuLCrV) is proposed”
The same questions in other papers
botanical · disease · Citrullus lanatus (watermelon)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
infection · disease · Citrullus lanatus (watermelon)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.