Abstract
Background and Objective: Watermelon (Citrullus lanatus) is a member of the Cucurbitaceae family native to Africa and has been cultivated since ancient times.Reports of chemo-resistance pathogens and health implications have stalled the application of agrochemicals for improving fruit production.This led to the isolation, purification, identification of pathogens and use of Azadirachta indica leaf extract to inhibit pathogenic growth responsible for post-harvest deterioration of watermelon (Citrullus lanatus).The study aims to isolate, purify and determine growth rate, zone of inhibition and colony count of fungi pathogen of watermelon using standard mycological techniques to ascertain the effectiveness of Azadirachta indica leaf extract on the pathogen.Materials and Methods: Sample collection and media preparation, culture and purification of isolates, pathogenicity test, as well as neem leaf aqueous extract, were conducted using standard pathological methods in this research, after which five fungi pathogens such as Aspergillus flavus, Fusarium oxysporum, Aspergillus niger, Rhizopus sp. and Saccharomyces sp.They were isolated from infested watermelon fruits and treated with 5, 10, 20 and 40 g concentrations of neem extracts where 5.4, 5.8, 6.1 and 6.6 cm inhibition rates were observed.Results: Aspergillus species appeared undeterred by the extract at these concentrations, but the overall inhibitory potential of neem leaf extracted was observed at 40 g inhibitory concentration at 50% concentration (IC 50 ).This means that the higher the concentration of the neem extract, the more effective in inhibiting pathogenic diseases of watermelon.Findings revealed that at controlled doses, Azadirachta indica leaf extracts may be able to suppress post-harvest pathogenic fungus in watermelon.Conclusion: To get compounds with significant resistance to post-harvest rotting, bioactive components should be assessed via bioassay-guided isolation.
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Claims 6
Claim · model-written summary
Five fungi were isolated from infested watermelon fruits.
- Taxon
- watermelon
Citrullus lanatuse0 - Interactor
- Aspergillus flavus
Aspergillus flavuse0pathogen - Outcome
- isolated from infested watermelon fruitse0
- Value
- fivee0
- Qualifier
- Fusarium oxysporum, Aspergillus niger, Rhizopus sp. and Saccharomyces sp.e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“five fungi pathogens such as Aspergillus flavus, Fusarium oxysporum, Aspergillus niger, Rhizopus sp. and Saccharomyces sp.They were isolated from infested watermelon fruits”
Claim · model-written summary
At neem extract doses of 5, 10, 20, and 40 g, inhibition measurements were 5.4, 5.8, 6.1, and 6.6 cm respectively.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- neem extractse0
- Outcome
- inhibition ratese0
- Value
- 5.4, 5.8, 6.1 and 6.6 cme0
- Qualifier
- 5, 10, 20 and 40 ge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treated with 5, 10, 20 and 40 g concentrations of neem extracts where 5.4, 5.8, 6.1 and 6.6 cm inhibition rates were observed”
t0“Watermelon (Citrullus lanatus)”
Claim · model-written summary
Aspergillus species were not deterred by neem extract at the tested concentrations.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- extracte0
- Interactor
- Aspergillus species
Aspergilluse0pathogen - Outcome
- Aspergillus species appeared undeterrede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aspergillus species appeared undeterred by the extract at these concentrations”
t0“Watermelon (Citrullus lanatus)”
Claim · model-written summary
The neem extract showed overall inhibitory potential at 40 g.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- neem leaf extractede0
- Outcome
- overall inhibitory potentiale0
- Value
- 40 ge0
- Qualifier
- 50% concentration (IC 50 )e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overall inhibitory potential of neem leaf extracted was observed at 40 g inhibitory concentration at 50% concentration (IC 50 )”
t0“Watermelon (Citrullus lanatus)”
Claim · model-written summary
Higher neem extract concentration inhibited watermelon pathogens more effectively.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- neem extracte0
- Outcome
- inhibiting pathogenic diseases of watermelone0
- Direction
- more effectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the higher the concentration of the neem extract, the more effective in inhibiting pathogenic diseases of watermelon”
Claim · model-written summary
The authors recommend bioassay-guided isolation of bioactive components for resistance to post-harvest rotting.
- Taxon
- WatermelonCitrullus lanatust0
- Outcome
- bioactive components should be assessed via bioassay-guided isolatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“To get compounds with significant resistance to post-harvest rotting, bioactive components should be assessed via bioassay-guided isolation”
t0“Watermelon (Citrullus lanatus)”
The same questions in other papers
botanical · disease · Citrullus lanatus (watermelon)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.