Abstract
ABSTRACT Broccoli (Brassica oleracea L. Botrytis Group ‘Green Duke’) seeds were germinated in vitro photoautotrophically (without sugar in medium) or photomixotrophically (with sugar in medium) for 3 weeks at 23 ° C and 150 μmol · m−2 · s−1 photosynthetic photon flux (PPF). Vessels were then stored at 1 ± 0.35°C under 1.6 ± 0.20,4.1 ± 0.35, or 8.6 ± 0.50 μmol · m−2 · s−1 constant PPF each of white (400–800 nm), red (600–700 nm), or blue (400–500 nm) light. Concentrations of CO2 inside the vessels were monitored until equilibrium was reached. Light compensation point was reached at $2.0 μmol · m−2 · s−1 for photoautotrophic seedlings and at $4.0 μmol · m−2 · s−1 for photomixotrophic seedlings. Therefore, in the long-term storage experiment, seedlings were stored for 4, 8, or 12 weeks at 1 ± 0.35°C in darkness or under 3 μmol · m−2 · s−1 constant PPF (average light compensation point) of white, red, or blue light. Variable to maximal chlorophyll fluorescence (Fv/Fm) of leaves decreased as storage time increased, regardless of media composition. Illumination during storage was necessary to maintain dry weight and regrowth potentials of in vitro seedlings. All photo-autotrophic seedlings stored in darkness were of poor quality and died when transferred to the greenhouse. Seventy-five percent of dark-stored photomixotrophic seedlings survived storage for 12 weeks but declined in appearance, dry weight, total soluble sugars (TSS), and chlorophyll fluorescence. Red light during storage increased seedling dry weight, TSS, and regrowth potential. Supplying 2% sucrose in the culture medium increased dry weight and maintained overall seedling quality during irradiated storage.
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Claims 9
Claim · model-written summary
Photoautotrophic seedlings reached light compensation at about 2.0 μmol · m−2 · s−1.
- Taxon
- Broccoli
Brassica oleracea var. italicat0 - Material
- photoautotrophic seedlingse0
- Outcome
- Light compensation pointe0
- Value
- $2.0 μmol · m−2 · s−1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light compensation point was reached at $2.0 μmol · m−2 · s−1 for photoautotrophic seedlings”
t0“Broccoli”
Claim · model-written summary
Photomixotrophic seedlings reached light compensation at about 4.0 μmol · m−2 · s−1.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Material
- photomixotrophic seedlingse0
- Outcome
- Light compensation pointe0
- Value
- $4.0 μmol · m−2 · s−1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light compensation point was reached at $2.0 μmol · m−2 · s−1 for photoautotrophic seedlings and at $4.0 μmol · m−2 · s−1 for photomixotrophic seedlings”
t0“Broccoli”
Claim · model-written summary
Leaf Fv/Fm decreased as storage time increased regardless of media composition.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Organ / stage
- leavese0
- Outcome
- Variable to maximal chlorophyll fluorescence (Fv/Fm)e0
- Direction
- decreasede0
- Setting
- storagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Variable to maximal chlorophyll fluorescence (Fv/Fm) of leaves decreased as storage time increased, regardless of media composition.”
t0“Broccoli”
Claim · model-written summary
Illumination during storage maintained seedling dry weight and regrowth potential.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- Illumination during storagee0
- Outcome
- dry weight and regrowth potentialse0
- Direction
- maintaine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Illumination during storage was necessary to maintain dry weight and regrowth potentials of in vitro seedlings.”
t0“Broccoli”
Claim · model-written summary
Dark-stored photoautotrophic seedlings were poor quality and died after greenhouse transfer.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Material
- photo-autotrophic seedlingse0
- Outcome
- poor quality and diede0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All photo-autotrophic seedlings stored in darkness were of poor quality and died when transferred to the greenhouse.”
t0“Broccoli”
Claim · model-written summary
Seventy-five percent of dark-stored photomixotrophic seedlings survived 12 weeks.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Material
- photomixotrophic seedlingse0
- Outcome
- survived storagee0
- Value
- Seventy-five percente0
- Qualifier
- 12 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seventy-five percent of dark-stored photomixotrophic seedlings survived storage for 12 weeks”
t0“Broccoli”
Claim · model-written summary
Dark-stored photomixotrophic seedlings declined in appearance, dry weight, TSS and chlorophyll fluorescence.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Outcome
- appearance, dry weight, total soluble sugars (TSS), and chlorophyll fluorescencee0
- Direction
- declinede0
- Setting
- storagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“photomixotrophic seedlings survived storage for 12 weeks but declined in appearance, dry weight, total soluble sugars (TSS), and chlorophyll fluorescence.”
t0“Broccoli”
Claim · model-written summary
Red storage light increased seedling dry weight, soluble sugars, and regrowth potential.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- Red light during storagee0
- Outcome
- seedling dry weight, TSS, and regrowth potentiale0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red light during storage increased seedling dry weight, TSS, and regrowth potential.”
t0“Broccoli”
Claim · model-written summary
Two-percent sucrose increased dry weight and maintained overall seedling quality in irradiated storage.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- 2% sucrosee0
- Outcome
- dry weight and maintained overall seedling qualitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Supplying 2% sucrose in the culture medium increased dry weight and maintained overall seedling quality during irradiated storage.”
t0“Broccoli”
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sucrose · quality: general · Brassica oleracea (wild cabbage)Results agree
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- positive
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6 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
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12 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- no effect
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See all 17 papers →light · growth · Brassica oleracea (wild cabbage)Positive and negative results
39 papers in the same direction as this paper · 13 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- no effect
50 papers more in this group
See all 54 papers →storage · physiology: osmolytes · Brassica oleracea (wild cabbage)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.