Paper · 1987 · New Phytologist

EFFECTS OF SIMULATED ACID RAIN ON PRODUCTION, MORPHOLOGY AND COMPOSITION OF EPICUTICULAR WAX AND ON CUTICULAR MEMBRANE DEVELOPMENT

K. E. PERCY; E. A. BAKER

20
claims
27
verbatim quotes
67
citations
2
research areas
✓
Open access

Abstract

S ummary Leaves of Phaseolus vulgaris L., Vicia faba L., Pisum sativum L. and Brassica napus L. were exposed to simulated rain from emergence to full expansion at seven pHs between 5.6 and 2.6, applied at 2 mm h −1 in amounts and at intervals representative of ambient rain in southwestern England. Leaf expansion was unaffected by rain pH greater than 30 in V. faba , and pH 2–6 in the other species. Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax. Foliar injury was greatest in species with crystalline wax. Wax production was affected by simulated acid rain in all species and was often accompanied by changes in wax composition. Wax quantity was reduced on leaves of P. vulgaris exposed to rain at pH 4.6, while wax on leaves of P. sativum and B. napus had increased numbers of smaller plates and tubes, respectively, per unit area at pH 4.6. Leaves of B. napus , stripped of crystalline epicuticular wax at about 20% full expansion, regenerated less wax at pH 3.4 than at pH 5.6. The thickness of the cuticular membranes in leaves of P. vulgaris, V faba and P. sativum exposed to simulated acid rain at pH 4.2 decreased by 28 to 35 % compared with those exposed to pH 5.6 rain. Membrane ultrastructure was also altered in leaves of P. vulgaris. Taken together, these changes could have important consequences for leaf wettability, rainfall retention, foliar uptake of chemicals and host‐parasite interactions.

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Claims 20

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Leaf expansion was unaffected under the reported rain pH condition in V. faba.

Taxon
V. fabaVicia fabae0
Treatment
rain pH greater than 30e0
Outcome
Leaf expansione0
Direction
unaffectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Leaf expansion was unaffected by rain pH greater than 30 in V. faba , and pH 2–6 in the other species.”

✓ located verbatim in the source · abstract, characters 299–401 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Leaf expansion was unaffected under the reported rain pH condition in P. vulgaris.

Taxon
P. vulgarisPhaseolus vulgarise1
Treatment
pH 2–6e0
Outcome
Leaf expansione0
Direction
unaffectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Leaf expansion was unaffected by rain pH greater than 30 in V. faba , and pH 2–6 in the other species.”

✓ located verbatim in the source · abstract, characters 299–401 · show in text ↑
e1

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Leaf expansion was unaffected under the reported rain pH condition in P. sativum.

Taxon
P. sativumLathyrus oleraceuse1
Treatment
pH 2–6e0
Outcome
Leaf expansione0
Direction
unaffectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Leaf expansion was unaffected by rain pH greater than 30 in V. faba , and pH 2–6 in the other species.”

✓ located verbatim in the source · abstract, characters 299–401 · show in text ↑
e1

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Leaf expansion was unaffected under the reported rain pH condition in B. napus.

Taxon
B. napusBrassica napuse1
Treatment
pH 2–6e0
Outcome
Leaf expansione0
Direction
unaffectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Leaf expansion was unaffected by rain pH greater than 30 in V. faba , and pH 2–6 in the other species.”

✓ located verbatim in the source · abstract, characters 299–401 · show in text ↑
e1

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Simulated acid rain induced foliar injury in P. sativum.

Taxon
P. sativumLathyrus oleraceuse0
Treatment
pH 3.4e0
Outcome
Macroscopic foliar injurye0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Simulated acid rain induced foliar injury in B. napus.

Taxon
B. napusBrassica napuse0
Treatment
pH 3.4e0
Outcome
Macroscopic foliar injurye0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Simulated acid rain induced foliar injury in P. vulgaris.

Taxon
P. vulgarisPhaseolus vulgarise0
Treatment
pH 3.0e0
Outcome
Macroscopic foliar injurye0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Simulated acid rain induced foliar injury in V. faba.

Taxon
V. fabaVicia fabae0
Treatment
pH 3.0e0
Outcome
Macroscopic foliar injurye0
Direction
inducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Foliar injury was greatest in species with crystalline wax.

Taxon
speciese0
Organ / stage
crystalline waxe0
Outcome
Foliar injurye0
Direction
greateste0
Qualifier
species with crystalline waxe0

Verbatim

e0

“Foliar injury was greatest in species with crystalline wax.”

✓ located verbatim in the source · abstract, characters 672–731 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Simulated acid rain affected wax production in P. vulgaris.

Taxon
P. vulgarisPhaseolus vulgarise1
Treatment
simulated acid raine0
Outcome
Wax productione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Wax production was affected by simulated acid rain in all species and was often accompanied by changes in wax composition.”

✓ located verbatim in the source · abstract, characters 732–854 · show in text ↑
e1

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Simulated acid rain affected wax production in V. faba.

Taxon
V. fabaVicia fabae1
Treatment
simulated acid raine0
Outcome
Wax productione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Wax production was affected by simulated acid rain in all species and was often accompanied by changes in wax composition.”

✓ located verbatim in the source · abstract, characters 732–854 · show in text ↑
e1

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Simulated acid rain affected wax production in P. sativum.

Taxon
P. sativumLathyrus oleraceuse1
Treatment
simulated acid raine0
Outcome
Wax productione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Wax production was affected by simulated acid rain in all species and was often accompanied by changes in wax composition.”

✓ located verbatim in the source · abstract, characters 732–854 · show in text ↑
e1

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Simulated acid rain affected wax production in B. napus.

Taxon
B. napusBrassica napuse1
Treatment
simulated acid raine0
Outcome
Wax productione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Wax production was affected by simulated acid rain in all species and was often accompanied by changes in wax composition.”

✓ located verbatim in the source · abstract, characters 732–854 · show in text ↑
e1

“Macroscopic foliar injury was induced at pH 3.4 in non‐wettable leaves of P. sativum and B. napus which had relatively large deposits of crystalline epicuticular wax, and at pH 3.0 in wettable leaves of P. vulgaris and V. faba which had small deposits of amorphous wax.”

✓ located verbatim in the source · abstract, characters 402–671 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Wax quantity was reduced in P. vulgaris at pH 4.6.

Taxon
P. vulgarisPhaseolus vulgarise0
Treatment
rain at pH 4.6e0
Outcome
Wax quantitye0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Wax quantity was reduced on leaves of P. vulgaris exposed to rain at pH 4.6, while wax on leaves of P. sativum and B. napus had increased numbers of smaller plates and tubes, respectively, per unit area at pH 4.6.”

✓ located verbatim in the source · abstract, characters 855–1068 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

P. sativum wax had increased numbers of smaller plates at pH 4.6.

Taxon
P. sativumLathyrus oleraceuse0
Treatment
pH 4.6e0
Outcome
numbers of smaller platese0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Wax quantity was reduced on leaves of P. vulgaris exposed to rain at pH 4.6, while wax on leaves of P. sativum and B. napus had increased numbers of smaller plates and tubes, respectively, per unit area at pH 4.6.”

✓ located verbatim in the source · abstract, characters 855–1068 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

B. napus wax had increased numbers of smaller tubes at pH 4.6.

Taxon
B. napusBrassica napuse0
Treatment
pH 4.6e0
Outcome
tubese0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Wax quantity was reduced on leaves of P. vulgaris exposed to rain at pH 4.6, while wax on leaves of P. sativum and B. napus had increased numbers of smaller plates and tubes, respectively, per unit area at pH 4.6.”

✓ located verbatim in the source · abstract, characters 855–1068 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Stripped B. napus leaves regenerated less wax at pH 3.4 than pH 5.6.

Taxon
B. napusBrassica napuse0
Treatment
pH 3.4e0
Outcome
regenerated less waxe0
Direction
lesse0
Comparator
pH 5.6e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Leaves of B. napus , stripped of crystalline epicuticular wax at about 20% full expansion, regenerated less wax at pH 3.4 than at pH 5.6.”

✓ located verbatim in the source · abstract, characters 1069–1206 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cuticular membrane thickness decreased by 28 to 35% at pH 4.2 versus pH 5.6 in P. vulgaris.

Taxon
P. vulgarisPhaseolus vulgarise0
Treatment
pH 4.2e0
Outcome
thickness of the cuticular membranese0
Direction
decreasede0
Value
28 to 35 %e0
Comparator
pH 5.6e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The thickness of the cuticular membranes in leaves of P. vulgaris, V faba and P. sativum exposed to simulated acid rain at pH 4.2 decreased by 28 to 35 % compared with those exposed to pH 5.6 rain.”

✓ located verbatim in the source · abstract, characters 1207–1404 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cuticular membrane thickness decreased by 28 to 35% at pH 4.2 versus pH 5.6 in V faba.

Taxon
V fabaVicia fabae0
Treatment
pH 4.2e0
Outcome
thickness of the cuticular membranese0
Direction
decreasede0
Value
28 to 35 %e0
Comparator
pH 5.6e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vicia faba
Vicia faba · TaxonRahmaziz · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The thickness of the cuticular membranes in leaves of P. vulgaris, V faba and P. sativum exposed to simulated acid rain at pH 4.2 decreased by 28 to 35 % compared with those exposed to pH 5.6 rain.”

✓ located verbatim in the source · abstract, characters 1207–1404 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cuticular membrane thickness decreased by 28 to 35% at pH 4.2 versus pH 5.6 in P. sativum.

Taxon
P. sativumLathyrus oleraceuse0
Treatment
pH 4.2e0
Outcome
thickness of the cuticular membranese0
Direction
decreasede0
Value
28 to 35 %e0
Comparator
pH 5.6e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The thickness of the cuticular membranes in leaves of P. vulgaris, V faba and P. sativum exposed to simulated acid rain at pH 4.2 decreased by 28 to 35 % compared with those exposed to pH 5.6 rain.”

✓ located verbatim in the source · abstract, characters 1207–1404 · show in text ↑

ph · growth · Lathyrus oleraceus (pea)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

ph · molecular: metabolites · Phaseolus vulgaris (common bean)Positive and negative results

7 papers: 4 positive, 3 negative or no effect

2 papers in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

ph · growth · Phaseolus vulgaris (common bean)Positive and negative results

9 papers: 3 positive, 6 negative or no effect

5 papers in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

ph · growth · Brassica napus (oilseed rape)Positive and negative results

10 papers: 6 positive, 4 negative or no effect

3 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

ph · crop injury · Lathyrus oleraceus (pea)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

ph · crop injury · Phaseolus vulgaris (common bean)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

ph · crop injury · Brassica napus (oilseed rape)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

rainfall · molecular: metabolites · Phaseolus vulgaris (common bean)Results agree

3 papers: 0 positive, 3 negative or no effect

2 papers in the same direction as this paper

  1. negative
  2. negative
See all 3 papers →

8 of 11 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.