Abstract
Abstract Rapeseed ( Brassica napus L.) is a major oilseed crop with high boron (B) requirements. In hydroponic cultivation, B uptake is highly responsive to the media conditions (e.g., pH). Different N sources, such as NH 4 + and NO 3 − , directly affect the media pH. To date, the underlying mechanisms, the role of the buffering at constant N-sources by separating the effects of pH and/or sources of N-form, for the uptake of B remain indistinct under hydroponic cultivation, particularly in rapeseed. Two identical experiments were conducted using (NH 4 ) 2 SO 4 and Ca(NO 3 ) 2 as sole N-sources each set were subjected to four treatments viz. non-buffered, buffered pH 5.0, 6.0, and 7.0 at low (1 µM) and high (100 µM) B for 5 days. The novelty of this study demonstrates that NH 4 + -fed plants prefer buffered pH (6 and 7) for promoting higher plant growth, which was in line with the B concentration, while NO 3 − -fed plants were not influenced. These aforesaid findings were further supported by the upregulation of BnaNIP5;1 and BnaBOR1;2 in the root and higher expression of BnaNIP5; 1 in shoots with buffered pH in NH 4 + -fed plants. According to our findings, in NH 4 + -based buffering, B transport channel protein BnaNIP5;1 (through diffusion) facilitated B uptake and distribution from root to shoot while BnaBOR1;2 mediated active uptake of B was in root under low supply of B. In a nutshell, pH buffering in hydroponics is very important in NH 4 + -based but not in NO 3 − -based nutrition for the uptake of boron and growth of the rapeseed plants.
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Claims 8
Claim · model-written summary
Buffered pH 6 and 7 increased growth of ammonium-fed rapeseed.
Not reportedComparator
Verbatim
e0“NH 4 + -fed plants prefer buffered pH (6 and 7) for promoting higher plant growth”
t0“Rapeseed (Brassica napus L.)”
Claim · model-written summary
Boron concentration followed growth under buffered ammonium nutrition.
Not reportedDirection
Verbatim
e0“which was in line with the B concentration”
e1“NH 4 + -fed plants prefer buffered pH (6 and 7) for promoting higher plant growth”
t0“Rapeseed (Brassica napus L.)”
Claim · model-written summary
Buffered pH did not influence nitrate-fed plants.
Not reportedComparator
Verbatim
e0“NO 3 − -fed plants were not influenced”
e1“buffered pH (6 and 7)”
t0“Rapeseed (Brassica napus L.)”
Claim · model-written summary
Buffered pH upregulated BnaNIP5;1 in roots of ammonium-fed plants.
Verbatim
e0“upregulation of BnaNIP5;1 and BnaBOR1;2 in the root”
e1“with buffered pH in NH 4 + -fed plants”
t0“Rapeseed (Brassica napus L.)”
Claim · model-written summary
Buffered pH upregulated BnaBOR1;2 in roots of ammonium-fed plants.
Verbatim
e0“upregulation of BnaNIP5;1 and BnaBOR1;2 in the root”
e1“with buffered pH in NH 4 + -fed plants”
t0“Rapeseed (Brassica napus L.)”
Claim · model-written summary
BnaNIP5; 1 expression was higher in shoots with buffered pH.
Verbatim
e0“higher expression of BnaNIP5; 1 in shoots with buffered pH in NH 4 + -fed plants”
t0“Rapeseed (Brassica napus L.)”
Claim · model-written summary
The authors attribute root-to-shoot boron uptake and distribution to BnaNIP5;1.
Verbatim
e0“B transport channel protein BnaNIP5;1 (through diffusion) facilitated B uptake and distribution from root to shoot”
t0“Rapeseed (Brassica napus L.)”
Claim · model-written summary
The authors attribute active root boron uptake under low boron supply to BnaBOR1;2.
Verbatim
e0“BnaBOR1;2 mediated active uptake of B was in root under low supply of B”
t0“Rapeseed (Brassica napus L.)”
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