Abstract
Th e eff ect of zygotic embryo age and pH of media on somatic embryogenesis induction of spring rapeseed was investigated. Immature zygotic embryos cultured on a growth regulator-free Murashige & Skoog medium produced primary somatic embryos without an intervening callus phase. Secondary somatic embryo induction was also possible on hormone-free medium. Similar to direct embryogenesis, the effi ciency of secondary embryogenesis depended on pH of media. Higher pH (5.0) stimulated the proliferation of primary somatic embryos 25 to 26 days aft er pollination of zygotic embryos, while a lower pH (3.5) signifi cantly increased the number of responding primary somatic embryos developed 20 to 21 days aft er pollination of zygotic embryos. Our study showed that primary somatic embryogenesis proceeded at a lower rate than secondary, but the highest number of somatic embryos per responding explant was obtained in primary somatic embryogenesis. Upon transfer to B5 medium, cotyledonary embryos developed into plantlets at a frequency of 7.3 - 30 %.
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Claims 7
Claim · model-written summary
Immature zygotic embryos on regulator free medium produced primary somatic embryos without callus.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Organ / stage
- Immature zygotic embryose0
- Treatment
- growth regulator-free Murashige & Skoog mediume0
- Outcome
- primary somatic embryos without an intervening callus phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Immature zygotic embryos cultured on a growth regulator-free Murashige & Skoog medium produced primary somatic embryos without an intervening callus phase.”
t0“Brassica napus”
Claim · model-written summary
Secondary somatic embryos could also be induced on hormone free medium.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- hormone-free mediume0
- Outcome
- Secondary somatic embryo inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Secondary somatic embryo induction was also possible on hormone-free medium.”
t0“Brassica napus”
Claim · model-written summary
Higher medium pH stimulated proliferation of older primary somatic embryos.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- Higher pH (5.0)e0
- Outcome
- proliferation of primary somatic embryose0
- Direction
- stimulatede0
- Qualifier
- 25 to 26 days aft er pollinatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher pH (5.0) stimulated the proliferation of primary somatic embryos 25 to 26 days aft er pollination of zygotic embryos”
t0“Brassica napus”
Claim · model-written summary
Lower pH significantly increased responding primary embryos at the earlier age.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- lower pH (3.5)e0
- Outcome
- number of responding primary somatic embryose0
- Direction
- increasede0
- Qualifier
- 20 to 21 days aft er pollinatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a lower pH (3.5) signifi cantly increased the number of responding primary somatic embryos developed 20 to 21 days aft er pollination of zygotic embryos”
t0“Brassica napus”
Claim · model-written summary
Primary somatic embryogenesis proceeded more slowly than secondary embryogenesis.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- ratee0
- Direction
- lowere0
- Comparator
- secondarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“primary somatic embryogenesis proceeded at a lower rate than secondary”
t0“Brassica napus”
Claim · model-written summary
Primary embryogenesis had the highest number of somatic embryos per responding explant.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- number of somatic embryos per responding explante0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest number of somatic embryos per responding explant was obtained in primary somatic embryogenesis.”
t0“Brassica napus”
Claim · model-written summary
Cotyledonary embryos developed into plantlets on B5 medium at 7.3 to 30% frequency.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- cotyledonary embryose0
- Treatment
- B5 mediume0
- Outcome
- developed into plantletse0
- Value
- 7.3 - 30 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon transfer to B5 medium, cotyledonary embryos developed into plantlets at a frequency of 7.3 - 30 %.”
t0“Brassica napus”
The same questions in other papers
ph · growth · Brassica napus (oilseed rape)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.