Abstract
Phytase from California Small White beans was extracted, concentrated and enriched by heat treatment and ammonium sulfate fractionation. Crude enzyme fractions tenaciously retained endogenous phytate which could be removed by autolysis at 45°C and pH 5.2. Characterization of the phytase preparation showed: (1) increase in activity with increasing temperature from 37 to 60°C, (2) an activation energy of approximately 9,200 cal/mole for the hydrolysis of inositol hexaphosphate; (3) pH optimum of 5.2; (4) a Km value of 2.22 × 10-4M; (5) apparently partial inhibition at high substrate concentrations; (6) fully competitive inhibition by one of the reaction products, inorganic phosphate, with Ki=3.1 × 104M. These properties and kinetic constants are comparable to those reported for phytase preparations from similar sources and they adequately account for in situ autolytic loss of phytic acid from these beans.
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Claims 8
Claim · model-written summary
Autolysis at 45°C and pH 5.2 removed endogenous phytate retained by crude bean phytase fractions.
- Taxon
- BEANSt0
- Treatment
- autolysis at 45°C and pH 5.2e0
- Gene
- phytaset0
- Outcome
- endogenous phytatee0
- Direction
- removede0
Not reportedComparator
Verbatim
e0“Crude enzyme fractions tenaciously retained endogenous phytate which could be removed by autolysis at 45°C and pH 5.2”
t0“CHARACTERIZATION OF PHYTASE OF BEANS (PHASEOLUS VULGARIS)”
Claim · model-written summary
Bean phytase activity increased as temperature rose from 37 to 60°C.
Verbatim
e0“increase in activity with increasing temperature from 37 to 60°C”
t0“CHARACTERIZATION OF PHYTASE OF BEANS (PHASEOLUS VULGARIS)”
Claim · model-written summary
The activation energy for bean phytase hydrolysis of inositol hexaphosphate was approximately 9,200 cal/mole.
Verbatim
e0“an activation energy of approximately 9,200 cal/mole for the hydrolysis of inositol hexaphosphate”
t0“CHARACTERIZATION OF PHYTASE OF BEANS (PHASEOLUS VULGARIS)”
Claim · model-written summary
The bean phytase pH optimum was 5.2.
Verbatim
e0“pH optimum of 5.2”
t0“CHARACTERIZATION OF PHYTASE OF BEANS (PHASEOLUS VULGARIS)”
Claim · model-written summary
The bean phytase Km was 2.22 × 10-4M.
Verbatim
e0“a Km value of 2.22 × 10-4M”
t0“CHARACTERIZATION OF PHYTASE OF BEANS (PHASEOLUS VULGARIS)”
Claim · model-written summary
High substrate concentrations partially inhibited bean phytase.
Verbatim
e0“apparently partial inhibition at high substrate concentrations”
t0“CHARACTERIZATION OF PHYTASE OF BEANS (PHASEOLUS VULGARIS)”
Claim · model-written summary
Inorganic phosphate competitively inhibited bean phytase, with Ki=3.1 × 104M.
- Taxon
- BEANSt0
- Treatment
- inorganic phosphatee0
- Gene
- PHYTASEt0
- Outcome
- competitive inhibitione0
- Direction
- inhibitione0
- Value
- Ki=3.1 × 104Me0
Not reportedComparator
Verbatim
e0“fully competitive inhibition by one of the reaction products, inorganic phosphate, with Ki=3.1 × 104M”
t0“CHARACTERIZATION OF PHYTASE OF BEANS (PHASEOLUS VULGARIS)”
Claim · model-written summary
The measured phytase properties account for in situ autolytic phytic acid loss from these beans.
Verbatim
e0“they adequately account for in situ autolytic loss of phytic acid from these beans”
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