Abstract
DNA methylation is a chromatin modification that is sometimes associated with epigenetic regulation of gene expression. As DNA methylation can be reversible at some loci, it is possible that methylation patterns may change within an organism that is subjected to environmental stress. In order to assess the effects of abiotic stress on DNA methylation patterns in maize (Zea mays), seeding plants were subjected to heat, cold, and UV stress treatments. Tissue was later collected from individual adult plants that had been subjected to stress or control treatments and used to perform DNA methylation profiling to determine whether there were consistent changes in DNA methylation triggered by specific stress treatments. DNA methylation profiling was performed by immunoprecipitation of methylated DNA followed by microarray hybridization to allow for quantitative estimates of DNA methylation abundance throughout the low-copy portion of the maize genome. By comparing the DNA methylation profiles of each individual plant to the average of the control plants it was possible to identify regions of the genome with variable DNA methylation. However, we did not find evidence of consistent DNA methylation changes resulting from the stress treatments used in this study. Instead, the data suggest that there is a low-rate of stochastic variation that is present in both control and stressed plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 3
Claim · model-written summary
Heat, cold and UV stress did not cause consistent DNA methylation changes in maize relative to controls.
- Treatment
- heat, cold, and UV stress treatmentse4
- Outcome
- consistent DNA methylation changese1
- Direction
- did not find evidence of consistente1
- Comparator
- the average of the control plantse2
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In order to assess the effects of abiotic stress on DNA methylation patterns in maize (Zea mays)”
e4“seeding plants were subjected to heat, cold, and UV stress treatments”
e1“we did not find evidence of consistent DNA methylation changes resulting from the stress treatments used in this study”
e2“By comparing the DNA methylation profiles of each individual plant to the average of the control plants it was possible to identify regions of the genome with variable DNA methylation”
Claim · model-written summary
A low rate of stochastic DNA methylation variation is present in both control and stressed maize plants.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In order to assess the effects of abiotic stress on DNA methylation patterns in maize (Zea mays)”
e3“the data suggest that there is a low-rate of stochastic variation that is present in both control and stressed plants”
Claim · model-written summary
Comparing each plant with the average of control plants identified regions of the maize genome with variable DNA methylation.
- Outcome
- regions of the genome with variable DNA methylatione2
- Comparator
- the average of the control plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In order to assess the effects of abiotic stress on DNA methylation patterns in maize (Zea mays)”
e2“By comparing the DNA methylation profiles of each individual plant to the average of the control plants it was possible to identify regions of the genome with variable DNA methylation”
The same questions in other papers
uv · molecular: expression · Zea mays (maize)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
heat · molecular: expression · Zea mays (maize)Positive and negative results
9 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
16 papers more in this group
See all 20 papers →cold · molecular: expression · Zea mays (maize)Positive and negative results
10 papers in the same direction as this paper · 21 in the opposite direction
- positive
- positive
- positive
28 papers more in this group
See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.