Abstract
Using three varieties of Brassica campestris, Hauarad (708), Maoshan-3 (714) and Youbai (715),as the maternal plants and one variety of Brassica oleracea Jingfeng-1 (6012) as paternal plants, crosses were made to produce interspecific hybrids through ovary culture techniques.The ovaries from the cross between B. campestris × B.oleracea (708 × 6012 and 714 × 6012) were cultured and ovary culture was more effective in terms of obtained seeds when ovaries were cultured in vitro at 9 d after pollination (DAP). While for the cross of 715 × 6012, it was better when ovaries in vitro cultured at 12 DAP. Among three cross combinations, the cross of 714 × 6012 showed the best response and 43 seeds per ovary were obtained. Among the media studied, the ovaries from the cross of 708 × 6012 cultured on MS media supplemented with 3.0 mg L-1 BA × 0.1 mg L-1 NAA showed better response, and its rate of seeds per ovary reached 44.0%.While the ovaries from the other two crosses (714 × 6012 and 715 × 6012) showed the best response when cultured on B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAA, and the rates of seeds per ovary reached 72.0 and 60.0%, respectively. All seeds obtained from the three cross combinations were cultured on the MS media supplemented with 1.0 mg L-1 BA + 0.05 mg L-1 NAA,and the seeds from the cross of 715 × 6012 showed the best germination response and the percentage of germinations reached 66.7%. The regenerated plantlets were obtained from these seedlings after cultured on the MS media supplemented with 0.05 mg L-1 NAA. Cytological study showed that these regenerated plants were all true hybrids of B.campestris × B.oleracea.
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Claims 9
Claim · model-written summary
For 708 × 6012 and 714 × 6012 crosses, ovary culture at 9 DAP was more effective for seed production.
- Taxon
- B. campestris × B.oleracea
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Brassica rapa subsp. oleiferae0 - Material
- 708 × 6012 and 714 × 6012e0
- Treatment
- cultured in vitro at 9 d after pollination (DAP)e0
- Outcome
- obtained seedse0
- Direction
- more effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The ovaries from the cross between B. campestris × B.oleracea (708 × 6012 and 714 × 6012) were cultured and ovary culture was more effective in terms of obtained seeds when ovaries were cultured in vitro at 9 d after pollination (DAP)”
Claim · model-written summary
For 715 × 6012, ovary culture at 12 DAP was better.
- Taxon
- Brassica campestrisBrassica rapa subsp. oleiferat0
- Material
- 715 × 6012e0
- Treatment
- ovaries in vitro cultured at 12 DAPe0
- Outcome
- bettere0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“for the cross of 715 × 6012, it was better when ovaries in vitro cultured at 12 DAP”
t0“Brassica campestris x B. oleracea”
Claim · model-written summary
The 714 × 6012 cross produced the best response at 43 seeds per ovary.
- Taxon
- Brassica campestrisBrassica rapa subsp. oleiferat0
- Material
- 714 × 6012e0
- Outcome
- seeds per ovarye0
- Direction
- beste0
- Value
- 43 seeds per ovarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cross of 714 × 6012 showed the best response and 43 seeds per ovary were obtained”
t0“Brassica campestris x B. oleracea”
Claim · model-written summary
The 708 × 6012 cross on supplemented MS medium achieved 44.0% seeds per ovary.
- Taxon
- Brassica campestrisBrassica rapa subsp. oleiferat0
- Material
- 708 × 6012e0
- Treatment
- MS media supplemented with 3.0 mg L-1 BA × 0.1 mg L-1 NAAe0
- Outcome
- rate of seeds per ovarye0
- Direction
- bettere0
- Value
- 44.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ovaries from the cross of 708 × 6012 cultured on MS media supplemented with 3.0 mg L-1 BA × 0.1 mg L-1 NAA showed better response, and its rate of seeds per ovary reached 44.0%”
t0“Brassica campestris x B. oleracea”
Claim · model-written summary
The 714 × 6012 cross on supplemented B5 medium achieved 72.0% seeds per ovary.
- Taxon
- Brassica campestrisBrassica rapa subsp. oleiferat0
- Material
- 714 × 6012e0
- Treatment
- B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAAe0
- Outcome
- rates of seeds per ovarye0
- Direction
- beste0
- Value
- 72.0e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ovaries from the other two crosses (714 × 6012 and 715 × 6012) showed the best response when cultured on B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAA, and the rates of seeds per ovary reached 72.0 and 60.0%, respectively”
t0“Brassica campestris x B. oleracea”
Claim · model-written summary
The 715 × 6012 cross on supplemented B5 medium achieved 60.0% seeds per ovary.
- Taxon
- Brassica campestrisBrassica rapa subsp. oleiferat0
- Material
- 715 × 6012e0
- Treatment
- B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAAe0
- Outcome
- rates of seeds per ovarye0
- Direction
- beste0
- Value
- 60.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ovaries from the other two crosses (714 × 6012 and 715 × 6012) showed the best response when cultured on B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAA, and the rates of seeds per ovary reached 72.0 and 60.0%, respectively”
t0“Brassica campestris x B. oleracea”
Claim · model-written summary
The 715 × 6012 seeds had the best germination at 66.7%.
- Taxon
- Brassica campestrisBrassica rapa subsp. oleiferat0
- Material
- 715 × 6012e0
- Outcome
- germination responsee0
- Direction
- beste0
- Value
- 66.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the seeds from the cross of 715 × 6012 showed the best germination response and the percentage of germinations reached 66.7%”
t0“Brassica campestris x B. oleracea”
Claim · model-written summary
Seedlings regenerated into plantlets on MS medium supplemented with NAA.
- Taxon
- Brassica campestrisBrassica rapa subsp. oleiferat0
- Treatment
- MS media supplemented with 0.05 mg L-1 NAAe0
- Outcome
- regenerated plantletse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regenerated plantlets were obtained from these seedlings after cultured on the MS media supplemented with 0.05 mg L-1 NAA”
t0“Brassica campestris x B. oleracea”
Claim · model-written summary
Cytology confirmed all regenerated plants as true interspecific hybrids.
- Taxon
- B.campestris × B.oleraceaBrassica rapa subsp. oleiferae0
- Outcome
- true hybridse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cytological study showed that these regenerated plants were all true hybrids of B.campestris × B.oleracea”
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- positive
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5 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.