Abstract
Using three varieties of Brassica campestris, Hauarad (708), Maoshan-3 (714) and Youbai (715),as the maternal plants and one variety of Brassica oleracea Jingfeng-1 (6012) as paternalplants, crosses were made to produce interspecific hybrids through ovary culture techniques.The ovaries from the cross between B. campestrisB.oleracea (7086012 and 7146012) werecultured and ovary culture was more effective in terms of obtained seeds when ovaries werecultured in vitro at 9 d after pollination (DAP). While for the cross of 7156012, it wasbetter when ovaries in vitro cultured at 12 DAP. Among three cross combinations, the cross of7146012 showed the best response and 43 seeds per ovary were obtained. Among the mediastudied, the ovaries from the cross of 7086012 cultured on MS media supplemented with 3.0 mgL-1 BA0.1 mg L-1 NAA showed better response, and its rate of seeds per ovary reached 44.0%.While the ovaries from the other two crosses (7146012 and 7156012) showed the best responsewhen cultured on B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAA, and the rates of seedsper ovary reached 72.0 and 60.0%, respectively. All seeds obtained from the three crosscombinations were cultured on the MS media supplemented with 1.0 mg L-1 BA + 0.05 mg L-1 NAA,and the seeds from the cross of 7156012 showed the best germination response and thepercentage of germinations reached 66.7%. The regenerated plantlets were obtained from theseseedlings after cultured on the MS media supplemented with 0.05 mg L-1 NAA. Cytological studyshowed that these regenerated plants were all true hybrids of B.campestrisB.oleracea.
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Claims 9
Claim · model-written summary
The 7086012 and 7146012 crosses produced more seeds when ovaries were cultured at 9 days after pollination.
- Taxon
- Brassica campestris x B. oleracea
.jpg?width=60)
Brassica rapa subsp. oleiferat0 - Material
- 7086012 and 7146012e1
- Treatment
- 9 d after pollination (DAP)e0
- Outcome
- obtained seedse0
- Direction
- more effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Brassica campestris x B. oleracea”
e0“ovary culture was more effective in terms of obtained seeds when ovaries werecultured in vitro at 9 d after pollination (DAP)”
e1“B. campestrisB.oleracea (7086012 and 7146012)”
Claim · model-written summary
The 7156012 cross performed better when ovaries were cultured at 12 days after pollination.
- Taxon
- Brassica campestris x B. oleraceaBrassica rapa subsp. oleiferat0
- Material
- 7156012e0
- Treatment
- 12 DAPe0
- Outcome
- bettere0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica campestris x B. oleracea”
e0“for the cross of 7156012, it wasbetter when ovaries in vitro cultured at 12 DAP”
Claim · model-written summary
The 7146012 cross had the best response, with 43 seeds per ovary.
- Taxon
- Brassica campestris x B. oleraceaBrassica rapa subsp. oleiferat0
- Material
- 7146012e0
- Outcome
- seeds per ovarye0
- Direction
- beste0
- Value
- 43 seeds per ovarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica campestris x B. oleracea”
e0“the cross of7146012 showed the best response and 43 seeds per ovary were obtained”
Claim · model-written summary
The 7086012 cross on supplemented MS medium had a 44.0% seeds per ovary rate.
- Taxon
- Brassica campestris x B. oleraceaBrassica rapa subsp. oleiferat0
- Material
- 7086012e0
- Treatment
- MS media supplemented with 3.0 mgL-1 BA0.1 mg L-1 NAAe0
- Outcome
- rate of seeds per ovarye0
- Direction
- bettere0
- Value
- 44.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica campestris x B. oleracea”
e0“the ovaries from the cross of 7086012 cultured on MS media supplemented with 3.0 mgL-1 BA0.1 mg L-1 NAA showed better response, and its rate of seeds per ovary reached 44.0%”
Claim · model-written summary
The 7146012 cross on supplemented B5 medium reached 72.0% seeds per ovary.
- Taxon
- Brassica campestris x B. oleraceaBrassica rapa subsp. oleiferat0
- Material
- 7146012e0
- Treatment
- B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAAe0
- Outcome
- rates of seedsper ovarye0
- Direction
- beste0
- Value
- 72.0e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica campestris x B. oleracea”
e0“the ovaries from the other two crosses (7146012 and 7156012) showed the best responsewhen cultured on B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAA, and the rates of seedsper ovary reached 72.0 and 60.0%, respectively”
Claim · model-written summary
The 7156012 cross on supplemented B5 medium reached 60.0% seeds per ovary.
- Taxon
- Brassica campestris x B. oleraceaBrassica rapa subsp. oleiferat0
- Material
- 7156012e0
- Treatment
- B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAAe0
- Outcome
- rates of seedsper ovarye0
- Direction
- beste0
- Value
- 60.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica campestris x B. oleracea”
e0“the ovaries from the other two crosses (7146012 and 7156012) showed the best responsewhen cultured on B5 media supplemented with 3.0 mg L-1 BA + 0.2 mg L-1 NAA, and the rates of seedsper ovary reached 72.0 and 60.0%, respectively”
Claim · model-written summary
Seeds from the 7156012 cross had the best germination response, reaching 66.7%.
- Taxon
- Brassica campestris x B. oleraceaBrassica rapa subsp. oleiferat0
- Material
- 7156012e0
- Outcome
- percentage of germinationse0
- Direction
- beste0
- Value
- 66.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica campestris x B. oleracea”
e0“the seeds from the cross of 7156012 showed the best germination response and thepercentage of germinations reached 66.7%”
Claim · model-written summary
Regenerated plantlets were obtained after seedlings were cultured on supplemented MS medium.
- Taxon
- Brassica campestris x B. oleraceaBrassica rapa subsp. oleiferat0
- Treatment
- MS media supplemented with 0.05 mg L-1 NAAe0
- Outcome
- regenerated plantletse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica campestris x B. oleracea”
e0“The regenerated plantlets were obtained from theseseedlings after cultured on the MS media supplemented with 0.05 mg L-1 NAA”
Claim · model-written summary
Cytology identified all regenerated plants as true interspecific hybrids.
- Taxon
- Brassica campestris x B. oleraceaBrassica rapa subsp. oleiferat0
- Outcome
- true hybridse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica campestris x B. oleracea”
e0“Cytological studyshowed that these regenerated plants were all true hybrids of B.campestrisB.oleracea”
The same questions in other papers
1-naphthaleneacetic acid · yield · Brassica rapa (field mustard)Results agree
1 paper in the same direction as this paper
- positive
in vitro · yield · Brassica rapa (field mustard)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.