Abstract
Studies on development in vitro of excised ovaries in the reciprocal crosses between Brassica campestris L. and Brassica oleracea L. were carried out. Three cultivars each of B. campestris and of B. oleracea were used in the experiment. Development in vitro of excised ovaries 4 days after pollination was studied on culture media with coconutmilk and casein hydrolysate, which were added either separately or in various combinations. Results obtained were as follows. (1) Many seeds were obtained in both sibmates of B. campestris and B. oleracea. Seed set in the capsules was not so different in the media used in the experiment. (2) Many seeds and naked-embryos protruded from undeveloped seed coat were obtained in the capsules of B. campestris× B. oleracea. Effect of coconut milk and casein hydrolysate in the medium on the production of hybrid plants was favorable but not indispensable in culture in vitro of excised ovaries. Fifteen hybrid plants were obtained in all ovaries cultured. (3) In the reciprocal cross, B. oleracea×B. campestris, no needs and no naked-embryos were obtained in all ovaries cultured. (4) Culture in vitro of excised ovaries is useful as a new technique for the production of interspecific hybrids in B. campestris×B. oleracea.
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Claims 7
Claim · model-written summary
Both parental sibmates produced many seeds.
- Taxon
- B. campestris and B. oleracea
Brassica oleracea.jpg?width=60)
Brassica rapa subsp. oleiferae0 - Outcome
- Many seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Many seeds were obtained in both sibmates of B. campestris and B. oleracea.”
Claim · model-written summary
Seed set was similar across tested media.
- Taxon
- Brassica campestris and Brassica oleraceaBrassica oleraceaBrassica rapa subsp. oleiferat0
- Treatment
- media used in the experimente0
- Outcome
- Seed set in the capsulese0
- Direction
- not so differente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seed set in the capsules was not so different in the media used in the experiment.”
t0“Brassica campestris and Brassica oleracea”
Claim · model-written summary
The B. campestris × B. oleracea cross yielded many seeds and naked embryos.
- Taxon
- B. campestris× B. oleraceaBrassica rapa subsp. oleiferae0
- Outcome
- Many seeds and naked-embryos protruded from undeveloped seed coate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many seeds and naked-embryos protruded from undeveloped seed coat were obtained in the capsules of B. campestris× B. oleracea.”
Claim · model-written summary
Coconut milk and casein hydrolysate favored hybrid production but were not indispensable.
- Taxon
- Brassica campestris and Brassica oleraceaBrassica oleraceaBrassica rapa subsp. oleiferat0
- Treatment
- coconut milk and casein hydrolysatee0
- Outcome
- production of hybrid plantse0
- Direction
- favorable but not indispensablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Effect of coconut milk and casein hydrolysate in the medium on the production of hybrid plants was favorable but not indispensable in culture in vitro of excised ovaries.”
t0“Brassica campestris and Brassica oleracea”
Claim · model-written summary
Fifteen hybrid plants arose from all cultured ovaries.
- Taxon
- Brassica campestris and Brassica oleraceaBrassica oleraceaBrassica rapa subsp. oleiferat0
- Outcome
- hybrid plantse0
- Value
- Fifteene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fifteen hybrid plants were obtained in all ovaries cultured.”
t0“Brassica campestris and Brassica oleracea”
Claim · model-written summary
The reciprocal B. oleracea × B. campestris cross yielded no seeds or naked embryos.
- Taxon
- B. oleracea×B. campestrisBrassica oleraceae0
- Outcome
- no needs and no naked-embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the reciprocal cross, B. oleracea×B. campestris, no needs and no naked-embryos were obtained in all ovaries cultured.”
Claim · model-written summary
Excised ovary culture was useful for producing B. campestris × B. oleracea hybrids.
- Taxon
- B. campestris×B. oleraceaBrassica rapa subsp. oleiferae0
- Outcome
- production of interspecific hybridse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Culture in vitro of excised ovaries is useful as a new technique for the production of interspecific hybrids in B. campestris×B. oleracea.”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.