Paper · 2025 · Applied Biosciences

Composition of Organic Fertilizers Containing Microorganisms and Their Effect on Soil Microbiological Activity and Plant Growth

Māris Seņkovs; Laila Dubova; Ina Alsiņa; et al.

10
claims
21
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

The conversion of livestock manure and peat into value-added fertilizers provides an environmentally sustainable approach to nutrient recycling and waste management. In this study, organic fertilizers were formulated from poultry, pig, and cattle manure mixed with peat and wood ash, with or without inoculation of the phosphate-solubilizing bacterium Priestia megaterium. Their efficiency was evaluated through plant growth and soil microbiological experiments involving conifer seedlings, herbaceous crops, and ornamental plants. Germination and growth trials with Norway spruce (Picea abies) and Scots pine (Pinus sylvestris) revealed clear species-specific responses: spruce seedlings performed best in substrates containing poultry or cattle manure, while pine showed enhanced growth with pig manure combined with bacterial inoculant. In pansies (Viola × wittrockiana), growth responses varied by cultivar; cattle manure enriched with bacteria increased leaf projection area, whereas poultry manure markedly suppressed growth. For cucumbers, basil, barley, radish, and garden beans, yields were lower than with mineral fertilizers, yet bacterial inoculation significantly influenced soil microbial activity by modifying respiration rates and hydrolytic enzyme intensity in plant- and manure-specific ways. The results demonstrate that microbial supplementation can alter soil biological processes and nutrient turnover, though its effects on plant productivity remain inconsistent. Further research is required to assess long-term performance under field conditions, as practical application will depend on achieving stable and reproducible results.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Spruce seedlings performed best in substrates containing poultry or cattle manure.

Taxon
Norway sprucePicea abiese1
Treatment
substrates containing poultry or cattle manuree2
Outcome
growthe1
Direction
performed beste2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Picea abies
Picea abies · TaxonIvar Leidus · CC BY-SA 3.0 ee · Wikimedia Commons ↗

Verbatim

e1

“Germination and growth trials with Norway spruce (Picea abies) and Scots pine (Pinus sylvestris) revealed clear species-specific responses”

✓ located verbatim in the source · abstract, characters 532–670 · show in text ↑
e2

“spruce seedlings performed best in substrates containing poultry or cattle manure”

✓ located verbatim in the source · abstract, characters 672–753 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Scots pine showed enhanced growth with pig manure combined with bacterial inoculant.

Taxon
Scots pinePinus sylvestrise1
Treatment
pig manure combined with bacterial inoculante2
Outcome
growthe1
Direction
enhancede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pinus sylvestris
Pinus sylvestris · TaxonHello, I am Bruce on Flickr · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e1

“Germination and growth trials with Norway spruce (Picea abies) and Scots pine (Pinus sylvestris) revealed clear species-specific responses”

✓ located verbatim in the source · abstract, characters 532–670 · show in text ↑
e2

“pine showed enhanced growth with pig manure combined with bacterial inoculant”

✓ located verbatim in the source · abstract, characters 761–838 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cattle manure enriched with bacteria increased leaf projection area in pansies.

Organ / stage
leafe2
Treatment
cattle manure enriched with bacteriae2
Outcome
leaf projection areae2
Direction
increasede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Viola x wittrockiana
Viola ×wittrockiana · Taxon · picture from its Wikimedia Commons categoryAndrew Bossi · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e1

“In pansies (Viola × wittrockiana), growth responses varied by cultivar”

✓ located verbatim in the source · abstract, characters 840–910 · show in text ↑
e2

“cattle manure enriched with bacteria increased leaf projection area”

✓ located verbatim in the source · abstract, characters 912–979 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Poultry manure markedly suppressed growth in pansies.

Treatment
poultry manuree2
Outcome
growthe2
Direction
suppressede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Viola x wittrockiana
Viola ×wittrockiana · Taxon · picture from its Wikimedia Commons categoryAndrew Bossi · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e1

“In pansies (Viola × wittrockiana), growth responses varied by cultivar”

✓ located verbatim in the source · abstract, characters 840–910 · show in text ↑
e2

“whereas poultry manure markedly suppressed growth”

✓ located verbatim in the source · abstract, characters 981–1030 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cucumber yields were lower with organic fertilizers than with mineral fertilizers.

Taxon
cucumbersCucumis sativuse1
Treatment
organic fertilizerse2
Outcome
yieldse1
Direction
lowere1
Comparator
mineral fertilizerse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“For cucumbers, basil, barley, radish, and garden beans, yields were lower than with mineral fertilizers”

✓ located verbatim in the source · abstract, characters 1032–1135 · show in text ↑
e2

“organic fertilizers were formulated from poultry, pig, and cattle manure mixed with peat and wood ash, with or without inoculation of the phosphate-solubilizing bacterium Priestia megaterium”

✓ located verbatim in the source · abstract, characters 181–371 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Basil yields were lower with organic fertilizers than with mineral fertilizers.

Treatment
organic fertilizerse2
Outcome
yieldse1
Direction
lowere1
Comparator
mineral fertilizerse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“For cucumbers, basil, barley, radish, and garden beans, yields were lower than with mineral fertilizers”

✓ located verbatim in the source · abstract, characters 1032–1135 · show in text ↑
e2

“organic fertilizers were formulated from poultry, pig, and cattle manure mixed with peat and wood ash, with or without inoculation of the phosphate-solubilizing bacterium Priestia megaterium”

✓ located verbatim in the source · abstract, characters 181–371 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Barley yields were lower with organic fertilizers than with mineral fertilizers.

Treatment
organic fertilizerse2
Outcome
yieldse1
Direction
lowere1
Comparator
mineral fertilizerse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“For cucumbers, basil, barley, radish, and garden beans, yields were lower than with mineral fertilizers”

✓ located verbatim in the source · abstract, characters 1032–1135 · show in text ↑
e2

“organic fertilizers were formulated from poultry, pig, and cattle manure mixed with peat and wood ash, with or without inoculation of the phosphate-solubilizing bacterium Priestia megaterium”

✓ located verbatim in the source · abstract, characters 181–371 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Radish yields were lower with organic fertilizers than with mineral fertilizers.

Treatment
organic fertilizerse2
Outcome
yieldse1
Direction
lowere1
Comparator
mineral fertilizerse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e1

“For cucumbers, basil, barley, radish, and garden beans, yields were lower than with mineral fertilizers”

✓ located verbatim in the source · abstract, characters 1032–1135 · show in text ↑
e2

“organic fertilizers were formulated from poultry, pig, and cattle manure mixed with peat and wood ash, with or without inoculation of the phosphate-solubilizing bacterium Priestia megaterium”

✓ located verbatim in the source · abstract, characters 181–371 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Garden bean yields were lower with organic fertilizers than with mineral fertilizers.

Taxon
garden beanse1
Treatment
organic fertilizerse2
Outcome
yieldse1
Direction
lowere1
Comparator
mineral fertilizerse1

Verbatim

e1

“For cucumbers, basil, barley, radish, and garden beans, yields were lower than with mineral fertilizers”

✓ located verbatim in the source · abstract, characters 1032–1135 · show in text ↑
e2

“organic fertilizers were formulated from poultry, pig, and cattle manure mixed with peat and wood ash, with or without inoculation of the phosphate-solubilizing bacterium Priestia megaterium”

✓ located verbatim in the source · abstract, characters 181–371 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Bacterial inoculation significantly influenced soil microbial activity, modifying respiration rates and hydrolytic enzyme intensity in plant- and manure-specific ways.

Taxon
herbaceous cropse3
Treatment
inoculation of the phosphate-solubilizing bacterium Priestia megateriume2
Outcome
soil microbial activitye1
Qualifier
respiration rates and hydrolytic enzyme intensitye1
Comparator
without inoculatione2

Not reportedDirection

Verbatim

e1

“bacterial inoculation significantly influenced soil microbial activity by modifying respiration rates and hydrolytic enzyme intensity in plant- and manure-specific ways”

✓ located verbatim in the source · abstract, characters 1141–1309 · show in text ↑
e2

“organic fertilizers were formulated from poultry, pig, and cattle manure mixed with peat and wood ash, with or without inoculation of the phosphate-solubilizing bacterium Priestia megaterium”

✓ located verbatim in the source · abstract, characters 181–371 · show in text ↑
e3

“involving conifer seedlings, herbaceous crops, and ornamental plants”

✓ located verbatim in the source · abstract, characters 462–530 · show in text ↑

fertilization · yield · Ocimum basilicum (basil)Results differ

52 papers: 48 positive, 4 negative or no effect

3 papers in the same direction as this paper · 48 in the opposite direction

  1. positive
  2. positive
  3. positive

48 papers more in this group

See all 52 papers →

fertilization · yield · Raphanus sativus (radish)Results differ

112 papers: 107 positive, 5 negative or no effect

4 papers in the same direction as this paper · 107 in the opposite direction

  1. positive
  2. positive
  3. positive

108 papers more in this group

See all 112 papers →

fertilization · growth · Viola x wittrockianaPositive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
See all 3 papers →

growing medium · growth · Picea abies (Norway spruce)Positive and negative results

8 papers: 5 positive, 3 negative or no effect

4 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. no effect
  3. no effect

4 papers more in this group

See all 8 papers →

inoculation · growth · Pinus sylvestris (Scots pine)Positive and negative results

18 papers: 17 positive, 1 negative or no effect

16 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

14 papers more in this group

See all 18 papers →

fertilization · growth · Picea abies (Norway spruce)Positive and negative results

19 papers: 12 positive, 7 negative or no effect

11 papers in the same direction as this paper · 7 in the opposite direction

  1. no effect
  2. no effect
  3. negative

15 papers more in this group

See all 19 papers →

fertilization · growth · Pinus sylvestris (Scots pine)Positive and negative results

33 papers: 24 positive, 9 negative or no effect

23 papers in the same direction as this paper · 8 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

29 papers more in this group

See all 33 papers →

fertilization · yield · Cucumis sativus (cucumber)Positive and negative results

288 papers: 263 positive, 25 negative or no effect

24 papers in the same direction as this paper · 263 in the opposite direction

  1. positive
  2. positive
  3. positive

284 papers more in this group

See all 288 papers →

8 of 9 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.