Abstract
This research developed an effective in-vitro method for regenerating strawberry plants, facilitating the large-scale production of planting materials. Various vegetative parts, such as runner tips, nodal segments, shoot apices, stems and leaves, were used for direct organogenesis. These explants were grown on MS media enriched with varying concentrations of plant growth regulators. The highest shoot multiplication rate has been found to be 89.8 % and the maximum number of shoots per explant (4.3) were achieved using runner tips in culture media supplemented with 1.5 mg/L BAP and 0.1 mg/L of NAA. The optimal conditions included pH of 5.8, 0.6 % agar, 3000 lux light intensity, 16-hr photoperiod and an incubation temperature of 25 ± 2 °C. The optimal percentage of rooting (90.8 %) for the regenerated shoots was achieved on ½ MS medium enriched with 1.0 mg/L IBA along with highest number of roots per culture. Subsequently. all regenerated plants were hardened in a greenhouse with 85 % relative humidity. To confirm the genetic fidelity of the in vitro-derived plants, 12 ISSR primers were used, revealing monomorphic bands. The standardized protocol developed in this study is crucial for large-scale multiplication and can also aid in identifying any clonal variations or adulteration.
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Claims 6
Claim · model-written summary
Runner tips on BAP and NAA gave an 89.8% shoot multiplication rate.
- Taxon
- Fragaria × ananassa
_single2.jpg?width=60)
Fragaria ×ananassat0 - Treatment
- runner tips in culture media supplemented with 1.5 mg/L BAP and 0.1 mg/L of NAAe0
- Outcome
- shoot multiplication ratee0
- Direction
- higheste0
- Value
- 89.8 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
t0“Fragaria × ananassa Duch. cv. Chandler”
e0“The highest shoot multiplication rate has been found to be 89.8 % and the maximum number of shoots per explant (4.3) were achieved using runner tips in culture media supplemented with 1.5 mg/L BAP and 0.1 mg/L of NAA”
Claim · model-written summary
Runner tips on BAP and NAA yielded 4.3 shoots per explant.
- Taxon
- Fragaria × ananassaFragaria ×ananassat0
- Treatment
- runner tips in culture media supplemented with 1.5 mg/L BAP and 0.1 mg/L of NAAe0
- Outcome
- number of shoots per explante0
- Direction
- maximume0
- Value
- 4.3e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fragaria × ananassa Duch. cv. Chandler”
e0“the maximum number of shoots per explant (4.3) were achieved using runner tips in culture media supplemented with 1.5 mg/L BAP and 0.1 mg/L of NAA”
Claim · model-written summary
Half-strength MS with IBA gave 90.8% rooting and the highest root number.
- Taxon
- Fragaria × ananassaFragaria ×ananassat0
- Organ / stage
- regenerated shootse0
- Treatment
- ½ MS medium enriched with 1.0 mg/L IBAe0
- Outcome
- percentage of rootinge0
- Direction
- optimale0
- Value
- 90.8 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fragaria × ananassa Duch. cv. Chandler”
e0“The optimal percentage of rooting (90.8 %) for the regenerated shoots was achieved on ½ MS medium enriched with 1.0 mg/L IBA along with highest number of roots per culture”
Claim · model-written summary
The regenerated plants were hardened in a greenhouse.
- Taxon
- Fragaria × ananassaFragaria ×ananassat0
- Outcome
- regenerated plants were hardenede0
- Qualifier
- 85 % relative humiditye0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fragaria × ananassa Duch. cv. Chandler”
e0“all regenerated plants were hardened in a greenhouse with 85 % relative humidity”
Claim · model-written summary
ISSR primers gave monomorphic bands in regenerated plants.
- Taxon
- Fragaria × ananassaFragaria ×ananassat0
- Outcome
- monomorphic bandse0
- Value
- 12 ISSR primerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fragaria × ananassa Duch. cv. Chandler”
e0“To confirm the genetic fidelity of the in vitro-derived plants, 12 ISSR primers were used, revealing monomorphic bands”
Claim · model-written summary
The authors consider the protocol useful for large-scale multiplication and detecting clonal variation.
- Taxon
- Fragaria × ananassaFragaria ×ananassat0
- Outcome
- large-scale multiplicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Fragaria × ananassa Duch. cv. Chandler”
e0“The standardized protocol developed in this study is crucial for large-scale multiplication and can also aid in identifying any clonal variations or adulteration”
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