Abstract
An efficient micropropagation system for strawberry cv. Camarosa was developed. Sterilized runner tips were cultured on hormone-free Murashige and Skoog (MS) medium with 3% sucrose, 1 mL·L −1 Plant Preservative Mixture, and solidified using 0.25% phytagel to produce in vitro stock plants. Shoot tips derived from the in vitro stock plants were cultured on MS media containing 0, 2, 4, and 8 μM thidiazuron (TDZ) and 0, 4, 9, 18, and 27 μM N6-benzylamino-purine (BAP) for shoot induction. Shoots produced on the best shoot induction medium were rooted on MS media containing 1, 2, 3, and 5 μM of either indole-3-butyric acid (IBA) or naphthaleneacetic acid (NAA). Results showed that MS medium with 2 μM TDZ and 4 μM BAP was optimum for shoot multiplication from the shoot tips. The most suitable medium for inducing the highest number of roots per explant, the highest percentage of explant with roots, and the highest mean root length were 1 μM NAA, 1 μM IBA, and hormone-free MS medium, respectively. Plantlets were transplanted into substrate consisting of perlite + vermiculite + cocopeat (2:1:2 v/v/v) resulting in 90% survival. After 1 month, plants were irrigated using Hoagland's solution and runners were produced after 3 months.
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Claims 6
Claim · model-written summary
MS medium with 2 μM TDZ and 4 μM BAP was optimum for shoot multiplication of strawberry cv. Camarosa.
- Taxon
- Strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Material
- cv. Camarosat0
- Treatment
- 2 μM TDZ and 4 μM BAPe0
- Outcome
- shoot multiplicatione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
t0“Micropropagation of Strawberry cv. Camarosa”
e0“MS medium with 2 μM TDZ and 4 μM BAP was optimum for shoot multiplication from the shoot tips”
Claim · model-written summary
The highest number of roots per explant was obtained on MS medium with 1 μM NAA.
- Taxon
- StrawberryFragaria ×ananassat0
- Material
- cv. Camarosat0
- Treatment
- 1 μM NAAe1
- Outcome
- highest number of roots per explante1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Strawberry cv. Camarosa”
e1“The most suitable medium for inducing the highest number of roots per explant, the highest percentage of explant with roots, and the highest mean root length were 1 μM NAA, 1 μM IBA, and hormone-free MS medium, respectively”
Claim · model-written summary
The highest percentage of explants with roots was obtained on MS medium with 1 μM IBA.
- Taxon
- StrawberryFragaria ×ananassat0
- Material
- cv. Camarosat0
- Treatment
- 1 μM IBAe1
- Outcome
- highest percentage of explant with rootse1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Strawberry cv. Camarosa”
e1“The most suitable medium for inducing the highest number of roots per explant, the highest percentage of explant with roots, and the highest mean root length were 1 μM NAA, 1 μM IBA, and hormone-free MS medium, respectively”
Claim · model-written summary
The highest mean root length was obtained on hormone-free MS medium.
- Taxon
- StrawberryFragaria ×ananassat0
- Material
- cv. Camarosat0
- Treatment
- hormone-free MS mediume1
- Outcome
- highest mean root lengthe1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Strawberry cv. Camarosa”
e1“The most suitable medium for inducing the highest number of roots per explant, the highest percentage of explant with roots, and the highest mean root length were 1 μM NAA, 1 μM IBA, and hormone-free MS medium, respectively”
Claim · model-written summary
Transplanted plantlets in perlite, vermiculite and cocopeat had 90% survival.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- perlite + vermiculite + cocopeat (2:1:2 v/v/v)e2
- Outcome
- survivale2
- Value
- 90%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Strawberry cv. Camarosa”
e2“Plantlets were transplanted into substrate consisting of perlite + vermiculite + cocopeat (2:1:2 v/v/v) resulting in 90% survival”
Claim · model-written summary
Plants irrigated with Hoagland solution produced runners after 3 months.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- Hoagland's solutione3
- Outcome
- runnerse3
- Qualifier
- after 3 monthse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Micropropagation of Strawberry cv. Camarosa”
e3“After 1 month, plants were irrigated using Hoagland's solution and runners were produced after 3 months”
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