Abstract
In vitro experiment was conducted at the Plant Tissue Culture Laboratory of the Department ofHorticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt during the period 2015-2016. Effects ofincreasing number of subcultures up to 12th subculture, BA concentrations up to 2.0 mg/l and their interaction onmultiplication rate of four strawberry cultivars were studied. Also, organogenic potential of leaf disc explants fromthree strawberry cultivars were tested using two different media (direct and indirect regeneration medium). Presentedresults showed that the highest multiplication rate in cvs. Kamela and Amega were achieved after the 6th subculturesusing 1.0 mg/l BA, however, the highest multiplication rate in cv. Camarosa was achieved after 7 subcultures using 1.0mg/ BA. The highest multiplication rate in cv. Fortuna was found after 6 subcultures on MS medium+0.5 mg/l BA,however, the lowest multiplication rate in all tested genotypes were after 12th subculture on MS+ 2.0 mg/l BA. Highregeneration capacity was obtained in medium amended with TDZ (direct regeneration medium) compared with 2,4-D+BA (indirect regeneration medium). Fortuna gave the highest regenerated shoots followed by cv. Kamela, then cv.Camarosa (v/v/v). Plantlets were successfully acclimated with 90% survival.
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Claims 7
Claim · model-written summary
Cultivars Kamela and Amega reached their highest multiplication rate after the 6th subculture with 1.0 mg/l BA.
- Taxon
- Strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Material
- Kamela and Amegae1
- Treatment
- 1.0 mg/l BAe1
- Outcome
- multiplication ratee1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
t0“Potential in Strawberry genotypes”
e1“the highest multiplication rate in cvs. Kamela and Amega were achieved after the 6th subculturesusing 1.0 mg/l BA”
Claim · model-written summary
Cultivar Camarosa reached its highest multiplication rate after 7 subcultures with 1.0 mg/l BA.
- Taxon
- StrawberryFragaria ×ananassat0
- Material
- Camarosae2
- Treatment
- 7 subcultures using 1.0mg/ BAe2
- Outcome
- multiplication ratee2
- Direction
- higheste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potential in Strawberry genotypes”
e2“the highest multiplication rate in cv. Camarosa was achieved after 7 subcultures using 1.0mg/ BA”
Claim · model-written summary
Cultivar Fortuna reached its highest multiplication rate after 6 subcultures on MS medium with 0.5 mg/l BA.
- Taxon
- StrawberryFragaria ×ananassat0
- Material
- Fortunae3
- Treatment
- MS medium+0.5 mg/l BAe3
- Outcome
- multiplication ratee3
- Direction
- higheste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potential in Strawberry genotypes”
e3“The highest multiplication rate in cv. Fortuna was found after 6 subcultures on MS medium+0.5 mg/l BA”
Claim · model-written summary
The lowest multiplication rate in all tested genotypes occurred after 12 subcultures on MS with 2.0 mg/l BA.
- Taxon
- StrawberryFragaria ×ananassat0
- Material
- all tested genotypese4
- Treatment
- 12th subculture on MS+ 2.0 mg/l BAe4
- Outcome
- multiplication ratee4
- Direction
- loweste4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potential in Strawberry genotypes”
e4“the lowest multiplication rate in all tested genotypes were after 12th subculture on MS+ 2.0 mg/l BA”
Claim · model-written summary
High regeneration capacity was obtained in medium with TDZ (direct regeneration) compared with 2,4-D+BA (indirect regeneration).
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- TDZ (direct regeneration medium)e5
- Outcome
- regeneration capacitye5
- Direction
- Highe5
- Comparator
- 2,4-D+BA (indirect regeneration medium)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potential in Strawberry genotypes”
e5“Highregeneration capacity was obtained in medium amended with TDZ (direct regeneration medium) compared with 2,4-D+BA (indirect regeneration medium)”
Claim · model-written summary
Cultivar Fortuna gave the highest number of regenerated shoots, followed by Kamela and then Camarosa.
- Taxon
- StrawberryFragaria ×ananassat0
- Material
- Fortunae6
- Outcome
- regenerated shootse6
- Direction
- higheste6
- Qualifier
- followed by cv. Kamela, then cv.Camarosae6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potential in Strawberry genotypes”
e6“Fortuna gave the highest regenerated shoots followed by cv. Kamela, then cv.Camarosa (v/v/v)”
Claim · model-written summary
Acclimated strawberry plantlets showed 90% survival.
- Taxon
- StrawberryFragaria ×ananassat0
- Organ / stage
- Plantletse7
- Outcome
- survivale7
- Value
- 90%e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potential in Strawberry genotypes”
e7“Plantlets were successfully acclimated with 90% survival”
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