Abstract
Introduction/Objective The aim of the study was to develop a technological approach for the production, storage, and preparation of strawberry microplants, facilitating their simultaneous acclimatization before planting in a greenhouse when favorable temperature and lighting conditions are established. Methods The initial material for the experiment was obtained via clonal micropropagation. Preparation, accumulation, and preservation of strawberry plants in vitro for delayed acclimatization were performed by periodically repeating the procedure of removing roots and leaves from previously rooted microplants and transferring them in the form of vegetating buds onto a fresh nutrient medium for further rehabilitation. The mass acclimatization of microplants was carried out under substrate-free flow-through hydroponic conditions, following which they were transplanted into a greenhouse in early spring. Results The survival rate of microplants during the periodically repeated procedures of removing roots and leaves from previously obtained microplants reached 100% for all studied cultivars. Active leaf and root recovery began on days 3–7 and 10–14, respectively. A 1-month acclimatization period on flow-through hydroponics allowed for a 2–2.5-fold increase in microplant height, a 2-fold increase in root length, and an almost 3-fold increase in weight. Discussion The repetitive removal of microplant roots and leaves, with their further transplantation on fresh nutrient medium as vegetating buds for subsequent rehabilitation during periods of unfavorable environmental conditions, does not reduce the viability of microplants, allowing the preservation of planting material without loss until acclimatization is advisable. Acclimatization using flow-through hydroponics enables the elimination of fungal pathogenesis that occurs during the use of solid substrates. This protocol also increases the predictability of acclimatization outcomes and ensures successful transplantation into a greenhouse. Conclusion The developed alternative strategy of strawberry clonal micropropagation allows prediction of the output volume of acclimatized microplants throughout a calendar year with a high degree of accuracy. The estimated quantity of such material can be compactly stored in a lighted tissue culture room, significantly saving time and resources. Microplants are well preserved until a favorable moment for acclimatization in a viable state as vegetating buds. After the rehabilitation cycles are complete, the microplants can be simultaneously transferred for acclimatization on flow-through hydroponics.
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Claims 12
Claim · model-written summary
Repeated root and leaf removal maintained 100% microplant survival across cultivars.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae1 - Treatment
- removing roots and leavese0
- Outcome
- survival rate of microplantse0
- Direction
- reachede0
- Value
- 100%e0
- Qualifier
- all studied cultivarse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The survival rate of microplants during the periodically repeated procedures of removing roots and leaves from previously obtained microplants reached 100% for all studied cultivars”
e1“strawberry plants in vitro”
Claim · model-written summary
Leaves began recovering on days 3–7 after rehabilitation.
- Taxon
- strawberryFragaria ×ananassae1
- Organ / stage
- leafe0
- Outcome
- leaf and root recoverye0
- Value
- days 3–7e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Active leaf and root recovery began on days 3–7 and 10–14, respectively”
e1“strawberry plants in vitro”
Claim · model-written summary
Roots began recovering on days 10–14 after rehabilitation.
- Taxon
- strawberryFragaria ×ananassae1
- Organ / stage
- roote0
- Outcome
- root recoverye0
- Value
- 10–14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Active leaf and root recovery began on days 3–7 and 10–14, respectively”
e1“strawberry plants in vitro”
Claim · model-written summary
One month of flow through hydroponic acclimatization increased microplant height 2–2.5 fold.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- 1-month acclimatization period on flow-through hydroponicse0
- Outcome
- microplant heighte0
- Direction
- increasee0
- Value
- 2–2.5-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 1-month acclimatization period on flow-through hydroponics allowed for a 2–2.5-fold increase in microplant height”
e1“strawberry plants in vitro”
Claim · model-written summary
One month of flow through hydroponic acclimatization doubled root length.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- 1-month acclimatization period on flow-through hydroponicse0
- Outcome
- root lengthe0
- Direction
- increasee0
- Value
- 2-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 1-month acclimatization period on flow-through hydroponics allowed for a 2–2.5-fold increase in microplant height, a 2-fold increase in root length”
e1“strawberry plants in vitro”
Claim · model-written summary
One month of flow through hydroponic acclimatization almost tripled microplant weight.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- 1-month acclimatization period on flow-through hydroponicse0
- Outcome
- weighte0
- Direction
- increasee0
- Value
- 3-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 1-month acclimatization period on flow-through hydroponics allowed for a 2–2.5-fold increase in microplant height, a 2-fold increase in root length, and an almost 3-fold increase in weight”
e1“strawberry plants in vitro”
Claim · model-written summary
The repeated bud rehabilitation procedure preserved microplant viability until acclimatization.
- Taxon
- strawberryFragaria ×ananassae1
- Outcome
- viability of microplantse0
- Comparator
- without losse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“does not reduce the viability of microplants, allowing the preservation of planting material without loss until acclimatization is advisable”
e1“strawberry plants in vitro”
Claim · model-written summary
Flow through hydroponics eliminated fungal pathogenesis associated with solid substrates.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- flow-through hydroponicse0
- Outcome
- fungal pathogenesise0
- Direction
- eliminatione0
- Comparator
- solid substratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acclimatization using flow-through hydroponics enables the elimination of fungal pathogenesis that occurs during the use of solid substrates”
e1“strawberry plants in vitro”
Claim · model-written summary
The protocol improved predictability of acclimatization outcomes.
- Taxon
- strawberryFragaria ×ananassae1
- Outcome
- predictability of acclimatization outcomese0
- Value
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This protocol also increases the predictability of acclimatization outcomes”
e1“strawberry plants in vitro”
Claim · model-written summary
The protocol supported successful greenhouse transplantation.
- Taxon
- strawberryFragaria ×ananassae1
- Outcome
- successful transplantation into a greenhousee0
- Comparator
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ensures successful transplantation into a greenhouse”
e1“strawberry plants in vitro”
Claim · model-written summary
The propagation strategy enabled accurate year round prediction of acclimatized microplant output.
- Taxon
- strawberryFragaria ×ananassae1
- Outcome
- output volume of acclimatized microplantse0
- Value
- high degree of accuracye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“allows prediction of the output volume of acclimatized microplants throughout a calendar year with a high degree of accuracy”
e1“strawberry clonal micropropagation”
Claim · model-written summary
Estimated planting material can be stored compactly with savings of time and resources.
- Taxon
- strawberryFragaria ×ananassae1
- Outcome
- saving time and resourcese0
- Comparator
- lighted tissue culture roome0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The estimated quantity of such material can be compactly stored in a lighted tissue culture room, significantly saving time and resources”
e1“strawberry clonal micropropagation”
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