Abstract
Summary Avocado is an important crop that would benefit from the production of new, superior cultivars; however, conventional breeding techniques for this species are slow. The development of cultivars would be aided if an efficient tissue culture system could be developed. This study describes the use of embryonic material and variations in tissue culture conditions on in vitro shoot development. The production of shoot bases from embryonic shoot tissues permitted the production of multiple adventitious shoots from one explant, with up to ten shoots developing from one base. Shoot development was significantly affected by the hormone regime on which the embryonic shoot bases were cultured. MMS medium supplemented with 1.0 mgl -1 BAP, 0.1 mg l -1 IBA, 0.1 mg l -1 GA 3 was found to be optimal for adventitious shoot development. Cutting the embryonic shoot tissue transversely significantly increased multiple shoot growth compared to longitudinal dissection. The addition of 0.1% PVP reduced tissue browning and necrosis. Root formation was achieved from 75% of adventitious microcuttings with the use of IBA and PUT, either as a pretreatment or in the culture medium. The combination of these methods should provide an efficient way of achieving multiple, adventitious shoot and root formation in vitro .
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Claims 6
Claim · model-written summary
Embryonic shoot bases yielded multiple adventitious shoots, up to ten per base.
- Taxon
- avocado
Persea americanat0 - Material
- embryonic shoot tissuese0
- Outcome
- multiple adventitious shootse0
- Value
- up to ten shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“avocado”
e0“The production of shoot bases from embryonic shoot tissues permitted the production of multiple adventitious shoots from one explant, with up to ten shoots developing from one base.”
Claim · model-written summary
Hormone regime significantly affected shoot development.
- Taxon
- avocadoPersea americanat0
- Treatment
- hormone regimee0
- Outcome
- Shoot developmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“avocado”
e0“Shoot development was significantly affected by the hormone regime on which the embryonic shoot bases were cultured.”
Claim · model-written summary
The supplemented MMS medium was optimal for adventitious shoot development.
- Taxon
- avocadoPersea americanat0
- Treatment
- MMS medium supplemented with 1.0 mgl -1 BAP, 0.1 mg l -1 IBA, 0.1 mg l -1 GA 3e0
- Outcome
- adventitious shoot developmente0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“avocado”
e0“MMS medium supplemented with 1.0 mgl -1 BAP, 0.1 mg l -1 IBA, 0.1 mg l -1 GA 3 was found to be optimal for adventitious shoot development.”
Claim · model-written summary
Transverse cutting increased multiple shoot growth versus longitudinal dissection.
- Taxon
- avocadoPersea americanat0
- Treatment
- Cutting the embryonic shoot tissue transverselye0
- Outcome
- multiple shoot growthe0
- Direction
- increasede0
- Comparator
- longitudinal dissectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“avocado”
e0“Cutting the embryonic shoot tissue transversely significantly increased multiple shoot growth compared to longitudinal dissection.”
Claim · model-written summary
PVP reduced tissue browning and necrosis.
- Taxon
- avocadoPersea americanat0
- Treatment
- 0.1% PVPe0
- Outcome
- tissue browning and necrosise0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“avocado”
e0“The addition of 0.1% PVP reduced tissue browning and necrosis.”
Claim · model-written summary
IBA and PUT enabled rooting of 75% of adventitious microcuttings.
- Taxon
- avocadoPersea americanat0
- Treatment
- IBA and PUTe0
- Outcome
- Root formatione0
- Value
- 75%e0
- Qualifier
- either as a pretreatment or in the culture mediume0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“avocado”
e0“Root formation was achieved from 75% of adventitious microcuttings with the use of IBA and PUT, either as a pretreatment or in the culture medium.”
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