Abstract
Advances in micropropagation technique have helped to produce true-to-type clones of many important horticulture plants. A procedure for micropropagation of Avocado was developed using stem segments as explant for the in vitro establishment and 60 to 100% of explants survived. The highest average shoot length was obtained on Murashing and Skoog (MS) basal medium supplemented with 1 mg/l 6-benzyl adenine (BA) and 0.5 mg/l naphthalene acetic acid (NAA). The maximum number of proliferated shoots (6.2 shoots/ explant) was obtained on MS medium supplemented with 2 mg/l BA, 0.5 mg/l NAA and 10 mg/l arginine. Meanwhile, the highest shoot length (3.7 cm) was obtained on MS medium free from growth regulators (control). Eighty percent of the shoots rooted on half strength MS medium supplemented with 4 mg/l indole butyric acid (IBA), 0.5 mg/l NAA and 160 mg/l phloroglucinol (PG). The complete plants with the maximum average root number / shoot (5) and length (9.3 cm) was obtained on medium containing 4 mg/l IBA, 0.5 mg/l NAA and 160 mg/l PG. The highest survival percentage of 70% was obtained from the plantlets when they were transferred to greenhouse conditions. Avocado can successfully micropropagated beginning with stem segments without significant damage to mother plant.
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Claims 7
Claim · model-written summary
Avocado stem-segment explants gave 60 to 100% survival in the in vitro establishment procedure.
- Taxon
- Avocado
Persea americanae1 - Organ / stage
- stem segmentse1
- Outcome
- explants survivede2
- Value
- 60 to 100%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“micropropagation of Avocado was developed using stem segments as explant”
e2“60 to 100% of explants survived”
Claim · model-written summary
MS basal medium with 1 mg/l BA and 0.5 mg/l NAA gave the highest average shoot length in avocado.
- Taxon
- AvocadoPersea americanae1
- Treatment
- Murashing and Skoog (MS) basal medium supplemented with 1 mg/l 6-benzyl adenine (BA) and 0.5 mg/l naphthalene acetic acid (NAA)e3
- Outcome
- average shoot lengthe3
- Direction
- higheste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“micropropagation of Avocado was developed using stem segments as explant”
e3“The highest average shoot length was obtained on Murashing and Skoog (MS) basal medium supplemented with 1 mg/l 6-benzyl adenine (BA) and 0.5 mg/l naphthalene acetic acid (NAA)”
Claim · model-written summary
MS medium with 2 mg/l BA, 0.5 mg/l NAA and 10 mg/l arginine gave the maximum number of proliferated avocado shoots, 6.2 per explant.
- Taxon
- AvocadoPersea americanae1
- Treatment
- 2 mg/l BA, 0.5 mg/l NAA and 10 mg/l argininee4
- Outcome
- number of proliferated shootse4
- Direction
- maximume4
- Value
- 6.2 shoots/ explante4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“micropropagation of Avocado was developed using stem segments as explant”
e4“The maximum number of proliferated shoots (6.2 shoots/ explant) was obtained on MS medium supplemented with 2 mg/l BA, 0.5 mg/l NAA and 10 mg/l arginine”
Claim · model-written summary
Medium free from growth regulators gave the highest avocado shoot length, 3.7 cm, among the media compared.
- Taxon
- AvocadoPersea americanae1
- Treatment
- free from growth regulatorse5
- Outcome
- shoot lengthe5
- Direction
- higheste5
- Value
- 3.7 cme5
- Comparator
- controle5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“micropropagation of Avocado was developed using stem segments as explant”
e5“Meanwhile, the highest shoot length (3.7 cm) was obtained on MS medium free from growth regulators (control)”
Claim · model-written summary
Eighty percent of avocado shoots rooted on half-strength MS medium with 4 mg/l IBA, 0.5 mg/l NAA and 160 mg/l phloroglucinol.
- Taxon
- AvocadoPersea americanae1
- Treatment
- 4 mg/l indole butyric acid (IBA), 0.5 mg/l NAA and 160 mg/l phloroglucinol (PG)e6
- Outcome
- shoots rootede6
- Direction
- rootede6
- Value
- Eighty percente6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“micropropagation of Avocado was developed using stem segments as explant”
e6“Eighty percent of the shoots rooted on half strength MS medium supplemented with 4 mg/l indole butyric acid (IBA), 0.5 mg/l NAA and 160 mg/l phloroglucinol (PG)”
Claim · model-written summary
Plantlets transferred to greenhouse conditions reached 70% survival, the highest survival percentage reported.
- Taxon
- AvocadoPersea americanae1
- Outcome
- survival percentagee2
- Value
- 70%e2
- Setting
- greenhouse conditionse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“micropropagation of Avocado was developed using stem segments as explant”
e2“The highest survival percentage of 70% was obtained from the plantlets when they were transferred to greenhouse conditions”
Claim · model-written summary
The authors conclude that avocado can be micropropagated from stem segments without significant damage to the mother plant.
- Taxon
- AvocadoPersea americanae2
- Organ / stage
- stem segmentse2
- Outcome
- micropropagatede2
- Qualifier
- without significant damage to mother plante2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Avocado can successfully micropropagated beginning with stem segments without significant damage to mother plant”
The same questions in other papers
indole-3-butyric acid · growth · Persea americana (avocado)Results differ
8 papers in the same direction as this paper · 2 in the opposite direction
- positive
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See all 7 papers →1-naphthaleneacetic acid · growth · Persea americana (avocado)Results agree
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1 paper in the same direction as this paper · 1 in the opposite direction
- negative
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in vitro · growth · Persea americana (avocado)Results agree
5 papers in the same direction as this paper
- positive
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.