Abstract
With global climate warming, enhancing the heat stress tolerance of rice seeds is critical for ensuring crop yields and maintaining global food security. 2,4-Epibrassionolide (EBR) has been shown to effectively alleviate the adverse effects of heat stress on rice seed germination, but its mitigation mechanism has not been fully clarified. In this experiment, exogenous EBR was used as a seed priming agent. The activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), malondialdehyde (MDA), soluble protein contents, and plant hormone levels were measured during rice seed germination under heat stress (38 °C). We constructed a cDNA library for transcriptome sequencing analysis. The results showed that exogenous EBR could effectively alleviate the effect of heat stress on rice seeds by enhancing SOD, POD, and CAT enzyme activity; reducing the MDA content; and increasing the soluble protein content. Additionally, exogenous EBR increases the levels of GA and IAA while decreasing the ABA content. According to a transcriptomic analysis, exogenous EBR can induce the expression of key genes involved in GA, IAA, and ABA hormone biosynthesis and metabolism, regulating GA-, IAA-, ABA-, and H2O2-mediated signaling pathways to promote the germination of rice seeds under heat stress. This study provides new insights into the application of rice seed priming techniques.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
EBR alleviated heat-stress effects on rice seeds.
- Taxon
- Rice
Oryza sativat0 - Treatment
- exogenous EBRe0
- Outcome
- effect of heat stress on rice seedse0
- Direction
- alleviatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous EBR could effectively alleviate the effect of heat stress on rice seeds”
t0“Rice”
Claim · model-written summary
EBR enhanced SOD, POD and CAT enzyme activity under heat stress.
- Taxon
- RiceOryza sativat0
- Treatment
- EBRe1
- Outcome
- SOD, POD, and CAT enzyme activitye0
- Direction
- enhancinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhancing SOD, POD, and CAT enzyme activity”
t0“Rice”
e1“The results showed that exogenous EBR could effectively alleviate”
Claim · model-written summary
EBR reduced MDA content.
- Taxon
- RiceOryza sativat0
- Treatment
- EBRe1
- Outcome
- MDA contente0
- Direction
- reducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reducing the MDA content”
t0“Rice”
e1“The results showed that exogenous EBR could effectively alleviate”
Claim · model-written summary
EBR increased soluble protein content.
- Taxon
- RiceOryza sativat0
- Treatment
- EBRe1
- Outcome
- soluble protein contente0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increasing the soluble protein content”
t0“Rice”
e1“The results showed that exogenous EBR could effectively alleviate”
Claim · model-written summary
EBR increased GA and IAA levels.
- Taxon
- RiceOryza sativat0
- Treatment
- exogenous EBRe0
- Outcome
- levels of GA and IAAe0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous EBR increases the levels of GA and IAA”
t0“Rice”
Claim · model-written summary
EBR decreased ABA content.
- Taxon
- RiceOryza sativat0
- Treatment
- EBRe1
- Outcome
- ABA contente0
- Direction
- decreasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreasing the ABA content”
t0“Rice”
e1“The results showed that exogenous EBR could effectively alleviate”
Claim · model-written summary
EBR induced expression of key genes in GA, IAA and ABA biosynthesis and metabolism.
- Taxon
- RiceOryza sativat0
- Treatment
- exogenous EBRe0
- Outcome
- expression of key genes involved in GA, IAA, and ABA hormone biosynthesis and metabolisme0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous EBR can induce the expression of key genes involved in GA, IAA, and ABA hormone biosynthesis and metabolism”
t0“Rice”
Claim · model-written summary
EBR was interpreted to regulate GA-, IAA-, ABA- and H2O2-mediated signaling to promote rice seed germination under heat stress.
- Taxon
- RiceOryza sativat0
- Outcome
- germination of rice seeds under heat stresse0
- Qualifier
- GA-, IAA-, ABA-, and H2O2-mediated signaling pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“regulating GA-, IAA-, ABA-, and H2O2-mediated signaling pathways to promote the germination of rice seeds under heat stress”
t0“Rice”
The same questions in other papers
24-epi-brassinolide · physiology: osmolytes · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
24-epi-brassinolide · physiology: hormones · Oryza sativa (rice)Results agree
2 papers in the same direction as this paper
- positive
- positive
24-epi-brassinolide · physiology: antioxidant enzymes · Oryza sativa (rice)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →24-epi-brassinolide · physiology: oxidative · Oryza sativa (rice)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →24-epi-brassinolide · molecular: metabolites · Oryza sativa (rice)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →24-epi-brassinolide · molecular: expression · Oryza sativa (rice)Results agree
8 papers in the same direction as this paper
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.