Abstract
SMALL AUXIN UP-REGULATED RNA (SAURs) genes are acknowledged as auxin-responsive genes that play crucial roles in modulating adaptive growth under abiotic stress conditions. Low temperatures constitute a primary limiting factor that significantly impairs the development, growth, and fruit quality of watermelon plants during the winter and spring seasons. Despite their potential importance, SAURs have not yet been thoroughly investigated or characterized in watermelon. In this study, we identified a positive regulator of the chilling stress response among watermelon SAURs, designated as ClSAUR1. Subcellular localization analysis demonstrated that the protein is directed to both the nucleus and cytoplasm. Quantitative real-time PCR (qRT-PCR) analysis indicated that ClSAUR1 is ubiquitously expressed across various watermelon tissues, with pronounced expression in the roots and leaves. Moreover, qRT-PCR and promoter::β-glucuronidase (GUS) staining assays revealed that the expression of ClSAUR1 is significantly upregulated in response to exogenous abscisic acid (ABA) and chilling stress. The overexpression of ClSAUR1 in tobacco lines was contrasted and analyzed, revealing an increased tolerance to chilling stress. This was evidenced by a reduced degree of wilting and chlorosis compared to wild-type (WT) plants. Furthermore, the overexpressed lines showed reduced reactive oxygen species (ROS) accumulation and increased antioxidant enzyme activity. The qRT-PCR results further indicated that the expression levels of genes associated with abscisic acid (ABA), antioxidant enzymes, and CBF–COR cold-responsive pathways were upregulated in the transgenic tobacco lines. This study provides new insights into the role of ClSAURs in enhancing the cold resistance of watermelon.
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Claims 9
Claim · model-written summary
ClSAUR1 protein localized to the nucleus and cytoplasm.
- Taxon
- Watermelon
Citrullus lanatust0 - Gene
- ClSAUR1t0
- Outcome
- both the nucleus and cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subcellular localization analysis demonstrated that the protein is directed to both the nucleus and cytoplasm.”
t0“A SMALL AUXIN UP-REGULATED RNA Gene Isolated from Watermelon (ClSAUR1)”
Claim · model-written summary
ClSAUR1 was expressed across watermelon tissues, particularly roots and leaves.
- Taxon
- watermelonCitrullus lanatuse0
- Organ / stage
- roots and leavese0
- Gene
- ClSAUR1e0
- Outcome
- ubiquitously expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ClSAUR1 is ubiquitously expressed across various watermelon tissues, with pronounced expression in the roots and leaves.”
Claim · model-written summary
Exogenous ABA significantly upregulated ClSAUR1 expression.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- exogenous abscisic acid (ABA)e0
- Gene
- ClSAUR1e0
- Outcome
- expression of ClSAUR1e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ClSAUR1 is significantly upregulated in response to exogenous abscisic acid (ABA) and chilling stress.”
t0“Watermelon”
Claim · model-written summary
Chilling stress significantly upregulated ClSAUR1 expression.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- chilling stresse0
- Gene
- ClSAUR1e0
- Outcome
- expression of ClSAUR1e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ClSAUR1 is significantly upregulated in response to exogenous abscisic acid (ABA) and chilling stress.”
t0“Watermelon”
Claim · model-written summary
ClSAUR1 overexpression increased tobacco chilling tolerance.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- overexpression of ClSAUR1e0
- Gene
- ClSAUR1e0
- Outcome
- tolerance to chilling stresse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of ClSAUR1 in tobacco lines was contrasted and analyzed, revealing an increased tolerance to chilling stress.”
Claim · model-written summary
ClSAUR1 overexpression reduced tobacco wilting and chlorosis compared with wild type.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- overexpression of ClSAUR1e0
- Gene
- ClSAUR1e0
- Outcome
- wilting and chlorosise0
- Direction
- reducede0
- Comparator
- wild-type (WT) plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of ClSAUR1 in tobacco lines was contrasted and analyzed, revealing an increased tolerance to chilling stress. This was evidenced by a reduced degree of wilting and chlorosis compared to wild-type (WT) plants.”
Claim · model-written summary
Transgenic tobacco lines accumulated less reactive oxygen species.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- overexpressed linese0
- Outcome
- reactive oxygen species (ROS) accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the overexpressed lines showed reduced reactive oxygen species (ROS) accumulation”
t0“tobacco”
Claim · model-written summary
Transgenic tobacco lines had greater antioxidant enzyme activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- overexpressed linese0
- Outcome
- antioxidant enzyme activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the overexpressed lines showed reduced reactive oxygen species (ROS) accumulation and increased antioxidant enzyme activity”
t0“tobacco”
Claim · model-written summary
Transgenic tobacco lines upregulated genes associated with ABA, antioxidant enzymes and CBF–COR pathways.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- transgenic tobacco linese0
- Outcome
- expression levels of genes associated with abscisic acid (ABA), antioxidant enzymes, and CBF–COR cold-responsive pathwayse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of genes associated with abscisic acid (ABA), antioxidant enzymes, and CBF–COR cold-responsive pathways were upregulated in the transgenic tobacco lines.”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
cold · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.