Abstract
Melatonin, a multifunctional signaling molecule, has been shown to play a significant role in response to abiotic stress. Several species have been reported to unveil melatonin’s effect on osmotic stress; however, the signal transduction mechanism of phytohormone-mediated melatonin biosynthesis in plant species remains unclear. In this study, although plants can biosynthesize melatonin, the exogenous application of melatonin to watermelon cells can improve cell growth in response to osmotic stress by regulating the antioxidant machinery of cells. Regarding the melatonin synthesis pathway, ClOMT (ClASMT and ClCOMT) is a multi-gene family, and ClSNAT has two members. Both ClOMTs and ClSNATs harbor the cis-elements in their promoter regions responding to various hormones. Among abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA), ABA treatment observably upregulated the expression of ClOMTs and ClSNATs, and the accumulation of melatonin with ABA treatment reached a level comparable to that with osmotic stress by mannitol treatment. Furthermore, when hormone biosynthesis inhibitors were added to cells before osmotic stress, the expression of ClOMTs and ClSNATs, as well as melatonin accumulation, were significantly suppressed with the ABA biosynthesis inhibitor. This study demonstrated the crucial role of melatonin biosynthesis in response to osmotic stress via plant hormone signal transduction. It showed that ABA signaling plays a dominant role in melatonin synthesis under osmotic stress.
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Claims 8
Claim · model-written summary
Exogenous melatonin improved watermelon cell growth under osmotic stress.
- Taxon
- Watermelon
Citrullus lanatust0 - Treatment
- exogenous application of melatonine0
- Outcome
- cell growthe0
- Direction
- improvee0
- Qualifier
- osmotic stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon”
e0“the exogenous application of melatonin to watermelon cells can improve cell growth in response to osmotic stress by regulating the antioxidant machinery of cells.”
Claim · model-written summary
ClOMT is a multi-gene family.
- Taxon
- WatermelonCitrullus lanatust0
- Gene
- ClOMTe0
- Outcome
- multi-gene familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon”
e0“ClOMT (ClASMT and ClCOMT) is a multi-gene family, and ClSNAT has two members.”
Claim · model-written summary
ClSNAT has two members.
- Taxon
- WatermelonCitrullus lanatust0
- Gene
- ClSNATe0
- Outcome
- two memberse0
- Value
- two memberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon”
e0“ClOMT (ClASMT and ClCOMT) is a multi-gene family, and ClSNAT has two members.”
Claim · model-written summary
ClOMTs and ClSNATs have hormone responsive promoter cis elements.
- Taxon
- WatermelonCitrullus lanatust0
- Gene
- ClOMTs and ClSNATse0
- Outcome
- cis-elements in their promoter regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon”
e0“Both ClOMTs and ClSNATs harbor the cis-elements in their promoter regions responding to various hormones.”
Claim · model-written summary
ABA upregulated ClOMT and ClSNAT expression.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- ABA treatmente0
- Gene
- ClOMTs and ClSNATse0
- Outcome
- expression of ClOMTs and ClSNATse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon”
e0“ABA treatment observably upregulated the expression of ClOMTs and ClSNATs, and the accumulation of melatonin with ABA treatment reached a level comparable to that with osmotic stress by mannitol treatment.”
Claim · model-written summary
ABA induced melatonin accumulation comparable to mannitol osmotic stress.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- ABA treatmente0
- Outcome
- accumulation of melatonine0
- Direction
- comparablee0
- Comparator
- osmotic stress by mannitol treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon”
e0“the accumulation of melatonin with ABA treatment reached a level comparable to that with osmotic stress by mannitol treatment.”
Claim · model-written summary
ABA biosynthesis inhibition significantly suppressed ClOMT and ClSNAT expression during osmotic stress.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- ABA biosynthesis inhibitore0
- Gene
- ClOMTs and ClSNATse0
- Outcome
- expression of ClOMTs and ClSNATse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon”
e0“the expression of ClOMTs and ClSNATs, as well as melatonin accumulation, were significantly suppressed with the ABA biosynthesis inhibitor.”
Claim · model-written summary
ABA biosynthesis inhibition significantly suppressed melatonin accumulation during osmotic stress.
- Taxon
- WatermelonCitrullus lanatust0
- Treatment
- ABA biosynthesis inhibitore0
- Outcome
- melatonin accumulatione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Watermelon”
e0“the expression of ClOMTs and ClSNATs, as well as melatonin accumulation, were significantly suppressed with the ABA biosynthesis inhibitor.”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.