Abstract
Cold stress signal transduction refers to a process that involves various physiological and molecular changes. Hydrogen peroxide (H2O2) signaling is stimulated by plants in adapting to various environmental stresses. Here, nine respiratory burst oxidase homolog (ClRboh) genes were identified from the watermelon genome. Chilling stress induced increased gene expression of ClRbohD and H2O2 accumulation in watermelon plants. By using virus-induced gene silencing (VIGS), we found that H2O2-induced chilling tolerance is dependent on ClRbohD by increased electrolyte leakage, membrane lipid peroxidation, and reduced photosystem II activity in ClRbohD-silenced plants compared with control. In addition, silencing of 2-Cys peroxiredoxin (Cl2-CP) increased the chilling sensitivity, while exogenous H2O2 treatment compromised the silencing of Cl2-CP-induced photooxidative damage in watermelon plants. The gene expression and enzyme activities of Cu/Zn-SOD and GR were significantly increased in Cl2-CP-silenced plants rather than pV190 control plants upon H2O2 treatment under chilling stress, suggesting that the activation of antioxidant enzymes by H2O2 serves as an alternative pathway to compensate for the deficiency of Cl2-CP during chilling stress response in watermelon.
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Claims 10
Claim · model-written summary
Nine ClRboh genes were identified in the watermelon genome.
- Taxon
- watermelon
Citrullus lanatuse0 - Gene
- ClRbohe0
- Outcome
- genese0
- Value
- ninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, nine respiratory burst oxidase homolog (ClRboh) genes were identified from the watermelon genome.”
Claim · model-written summary
Chilling increased ClRbohD expression in watermelon.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- Chilling stresse0
- Gene
- ClRbohDe0
- Outcome
- gene expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chilling stress induced increased gene expression of ClRbohD and H2O2 accumulation in watermelon plants.”
Claim · model-written summary
Chilling increased H2O2 accumulation in watermelon.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- Chilling stresse0
- Outcome
- H2O2 accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chilling stress induced increased gene expression of ClRbohD and H2O2 accumulation in watermelon plants.”
Claim · model-written summary
ClRbohD silencing increased electrolyte leakage during H2O2-induced chilling tolerance.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- ClRbohD-silenced plantse0
- Gene
- ClRbohDe0
- Outcome
- electrolyte leakagee0
- Direction
- increasede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2O2-induced chilling tolerance is dependent on ClRbohD by increased electrolyte leakage, membrane lipid peroxidation, and reduced photosystem II activity in ClRbohD-silenced plants compared with control.”
t0“watermelon”
Claim · model-written summary
ClRbohD silencing increased membrane lipid peroxidation during H2O2-induced chilling tolerance.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- ClRbohD-silenced plantse0
- Gene
- ClRbohDe0
- Outcome
- membrane lipid peroxidatione0
- Direction
- increasede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2O2-induced chilling tolerance is dependent on ClRbohD by increased electrolyte leakage, membrane lipid peroxidation, and reduced photosystem II activity in ClRbohD-silenced plants compared with control.”
t0“watermelon”
Claim · model-written summary
ClRbohD silencing reduced photosystem II activity during H2O2-induced chilling tolerance.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- ClRbohD-silenced plantse0
- Gene
- ClRbohDe0
- Outcome
- photosystem II activitye0
- Direction
- reducede0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2O2-induced chilling tolerance is dependent on ClRbohD by increased electrolyte leakage, membrane lipid peroxidation, and reduced photosystem II activity in ClRbohD-silenced plants compared with control.”
t0“watermelon”
Claim · model-written summary
Cl2-CP silencing increased chilling sensitivity in watermelon.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- silencing of 2-Cys peroxiredoxin (Cl2-CP)e0
- Gene
- Cl2-CPe0
- Outcome
- chilling sensitivitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of 2-Cys peroxiredoxin (Cl2-CP) increased the chilling sensitivity, while exogenous H2O2 treatment compromised the silencing of Cl2-CP-induced photooxidative damage in watermelon plants.”
Claim · model-written summary
Exogenous H2O2 reduced photooxidative damage induced by Cl2-CP silencing in watermelon.
- Taxon
- watermelonCitrullus lanatuse0
- Treatment
- exogenous H2O2 treatmente0
- Gene
- Cl2-CPe0
- Outcome
- photooxidative damagee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of 2-Cys peroxiredoxin (Cl2-CP) increased the chilling sensitivity, while exogenous H2O2 treatment compromised the silencing of Cl2-CP-induced photooxidative damage in watermelon plants.”
Claim · model-written summary
H2O2 treatment under chilling increased Cu/Zn-SOD gene expression and enzyme activity in Cl2-CP-silenced plants relative to controls.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- H2O2 treatmente0
- Gene
- Cu/Zn-SODe0
- Outcome
- gene expression and enzyme activitiese0
- Direction
- increasede0
- Qualifier
- under chilling stresse0
- Comparator
- pV190 control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene expression and enzyme activities of Cu/Zn-SOD and GR were significantly increased in Cl2-CP-silenced plants rather than pV190 control plants upon H2O2 treatment under chilling stress”
t0“watermelon”
Claim · model-written summary
H2O2 treatment under chilling increased GR gene expression and enzyme activity in Cl2-CP-silenced plants relative to controls.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- H2O2 treatmente0
- Gene
- GRe0
- Outcome
- gene expression and enzyme activitiese0
- Direction
- increasede0
- Qualifier
- under chilling stresse0
- Comparator
- pV190 control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene expression and enzyme activities of Cu/Zn-SOD and GR were significantly increased in Cl2-CP-silenced plants rather than pV190 control plants upon H2O2 treatment under chilling stress”
t0“watermelon”
The same questions in other papers
cold · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
5 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.