Abstract
Heat-shock protein 20s (HSP20) were initially shown to play a role during heat shock stress; however, recent data indicated that HSP20 proteins are also involved in abiotic stress in plants. Watermelon is known to be vulnerable to various stressors; however, HSP20 proteins have yet to be investigated and characterized in the watermelon. In a previous study, we identified a negative regulator of salt stress response from watermelon: ClHSP22.8 , a member of the HSP20 family. Quantitative real-time PCR (qRT-PCR) and promoter::β-glucuronidase ( GUS ) analysis revealed that ClHSP22.8 was expressed widely in a range of different tissues from the watermelon, but particularly in the roots of 7-day-old seedlings and flowers. Furthermore, qRT-PCR and GUS staining showed that the expression of ClHSP22.8 was significantly repressed by exogenous abscisic acid (ABA) and salt stress. The over-expression of ClHSP22.8 in Arabidopsis lines resulted in hypersensitivity to ABA and reduced tolerance to salt stress. Furthermore, the expression patterns of key regulators associated with ABA-dependent and independent pathways, and other stress-responsive signaling pathways, were also repressed in transgenic lines that over-expressed ClHSP22.8 . These results indicated that ClHSP22.8 is a negative regulator in plant response to salt stress and occurs via ABA-dependent and independent, and other stress-responsive signaling pathways.
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Claims 7
Claim · model-written summary
ClHSP22.8 was widely expressed in watermelon tissues, especially seedling roots and flowers.
- Taxon
- watermelon
Citrullus lanatuse0 - Organ / stage
- roots of 7-day-old seedlings and flowerse0
- Gene
- ClHSP22.8e0
- Outcome
- expressed widelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative real-time PCR (qRT-PCR) and promoter::β-glucuronidase ( GUS ) analysis revealed that ClHSP22.8 was expressed widely in a range of different tissues from the watermelon, but particularly in the roots of 7-day-old seedlings and flowers”
Claim · model-written summary
exogenous abscisic acid (ABA) significantly repressed ClHSP22.8 expression.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- exogenous abscisic acid (ABA)e0
- Gene
- ClHSP22.8e0
- Outcome
- expression of ClHSP22.8e0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“qRT-PCR and GUS staining showed that the expression of ClHSP22.8 was significantly repressed by exogenous abscisic acid (ABA) and salt stress”
t0“watermelon”
Claim · model-written summary
salt stress significantly repressed ClHSP22.8 expression.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- salt stresse0
- Gene
- ClHSP22.8e0
- Outcome
- expression of ClHSP22.8e0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“qRT-PCR and GUS staining showed that the expression of ClHSP22.8 was significantly repressed by exogenous abscisic acid (ABA) and salt stress”
t0“watermelon”
Claim · model-written summary
ClHSP22.8 overexpression caused ABA hypersensitivity in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- over-expression of ClHSP22.8e0
- Gene
- ClHSP22.8e0
- Outcome
- hypersensitivity to ABAe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of ClHSP22.8 in Arabidopsis lines resulted in hypersensitivity to ABA and reduced tolerance to salt stress”
Claim · model-written summary
ClHSP22.8 overexpression reduced salt-stress tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- over-expression of ClHSP22.8e0
- Gene
- ClHSP22.8e0
- Outcome
- tolerance to salt stresse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of ClHSP22.8 in Arabidopsis lines resulted in hypersensitivity to ABA and reduced tolerance to salt stress”
Claim · model-written summary
ClHSP22.8 overexpression repressed regulators of ABA-dependent and independent and other stress-response pathways.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- over-expressed ClHSP22.8e0
- Gene
- ClHSP22.8e0
- Outcome
- expression patterns of key regulators associated with ABA-dependent and independent pathways, and other stress-responsive signaling pathwayse0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression patterns of key regulators associated with ABA-dependent and independent pathways, and other stress-responsive signaling pathways, were also repressed in transgenic lines that over-expressed ClHSP22.8”
t0“Arabidopsis”
Claim · model-written summary
The authors infer that ClHSP22.8 negatively regulates salt-stress response through several signaling pathways.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ClHSP22.8e0
- Outcome
- plant response to salt stresse0
- Qualifier
- ABA-dependent and independent, and other stress-responsive signaling pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results indicated that ClHSP22.8 is a negative regulator in plant response to salt stress and occurs via ABA-dependent and independent, and other stress-responsive signaling pathways”
t0“Arabidopsis”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
salinity · molecular: expression · Citrullus lanatus (watermelon)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.