Abstract
Ralstonia solanacearum releases a set of effectors into plant cells that modify the host defence reaction. The role of the effector protein RipI during infection has not been elucidated. In this study, we demonstrated that transient overexpression of RipI induces the hypersensitive response (HR), up-regulating the HR marker gene hin1, in Nicotiana benthamiana. Deletion of R. solanacearum ripI led to increased virulence in tomato (Solanum lycopersicum) plants. Through yeast two-hybrid and pull-down assays, we identified an interaction between the N. benthamiana transcription factor bHLH93 and RipI, both of which could be localized in the nucleus of Arabidopsis protoplasts. Silencing of bHLH93 markedly attenuated the RipI-induced HR and induced expression of the PDF1.2 defence gene. These data demonstrate that the R. solanacearum effector RipI induces a host defence reaction by interacting with the bHLH93 transcription factor.
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Claims 6
Claim · model-written summary
RipI overexpression induced hypersensitive response and upregulated hin1 in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- transient overexpression of RipIe0
- Interactor
- Ralstonia solanacearum
Ralstonia solanacearumt0pathogen - Gene
- RipIe0
- Outcome
- hypersensitive response (HR), up-regulating the HR marker gene hin1e0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of RipI induces the hypersensitive response (HR), up-regulating the HR marker gene hin1, in Nicotiana benthamiana”
t0“Ralstonia solanacearum”
Claim · model-written summary
Deleting ripI increased Ralstonia virulence in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- Deletion of R. solanacearum ripIe0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Gene
- R. solanacearum ripIe0
- Outcome
- virulencee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of R. solanacearum ripI led to increased virulence in tomato (Solanum lycopersicum) plants.”
Claim · model-written summary
Plant bHLH93 interacted with RipI.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- Ralstonia solanacearumRalstonia solanacearumt0pathogen
- Gene
- bHLH93e0
- Outcome
- interaction between the N. benthamiana transcription factor bHLH93 and RipIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified an interaction between the N. benthamiana transcription factor bHLH93 and RipI”
t0“Ralstonia solanacearum”
Claim · model-written summary
bHLH93 and RipI localized in Arabidopsis protoplast nuclei.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- protoplastse0
- Gene
- bHLH93t0
- Outcome
- localized in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both of which could be localized in the nucleus of Arabidopsis protoplasts”
t0“bHLH93”
Claim · model-written summary
Silencing bHLH93 attenuated the RipI-induced hypersensitive response.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of bHLH93e0
- Interactor
- Ralstonia solanacearumRalstonia solanacearumt0pathogen
- Gene
- bHLH93e0
- Outcome
- RipI-induced HRe0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of bHLH93 markedly attenuated the RipI-induced HR”
t0“Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana”
Claim · model-written summary
Silencing bHLH93 induced PDF1.2 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of bHLH93e0
- Interactor
- Ralstonia solanacearumRalstonia solanacearumt0pathogen
- Gene
- PDF1.2e0
- Outcome
- expression of the PDF1.2 defence genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of bHLH93 markedly attenuated the RipI-induced HR and induced expression of the PDF1.2 defence gene.”
t0“Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.