Paper · 2022 · IOP Conference Series Earth and Environmental Science

Optimization of benzyl amino purines (BAP) concentration and medium type on the induction of banana shoots (Musa acuminata Colla.) cv. Barangan Merah under in vitro condition

M Setyowati; Efendi; Alfizar; et al.

5
claims
10
verbatim quotes
3
citations
2
research areas
✓
Open access · cc-by

Abstract

Abstract The use of optimum concentration of BAP and the right medium type can support shoot induction on the explant of banana sucker cv.Barangan Merah. The Research was conducted at the Laboratory of Tissue Culture, Faculty of Agriculture, Universitas Syiah Kuala. This research used a completely randomized 3x3 factorial design. The first factor was Benzyl Amino Purines (BAP) concentration at three levels of concentration, i.e. control, 3 mg.L -1 , and 6 mg.L -1 . The second factor was Musrahige & Skoog (MS) medium type at three levels, i.e. solid, solid-liquid, and liquid. Results showed that the BAP treatment of 3 mg.L -1 had the biggest response to the number of open midribs compared to other BAP treatments. The type of solid medium tends to shoot induction better than other types of medium. The contamination that occurred was 13.9% of the 72 explants planted. The contamination was caused by Mucor and Aspergillus fungi. The bacteria causing the contamination were gram positive bacteria (coccus) and gram-negative bacteria (coccus and bacilli).

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BAP at 3 mg/L gave the biggest response in number of open midribs among BAP treatments in banana shoot induction.

Taxon
bananat0
Material
Barangan Meraht0
Treatment
BAP treatment of 3 mg.L -1e1
Outcome
number of open midribse1
Direction
biggeste1
Comparator
other BAP treatmentse1
Setting
in vitro conditiont0

Verbatim

e1

“Results showed that the BAP treatment of 3 mg.L -1 had the biggest response to the number of open midribs compared to other BAP treatments”

✓ located verbatim in the source · abstract, characters 582–720 · show in text ↑
t0

“banana shoots (Musa acuminata Colla.) cv. Barangan Merah under in vitro condition”

✓ located verbatim in the source · title, characters 93–174 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Solid medium tends to give better shoot induction than other medium types in banana.

Taxon
bananat0
Treatment
type of solid mediume2
Outcome
shoot inductione2
Direction
bettere2
Qualifier
tends toe2
Comparator
other types of mediume2
Setting
in vitro conditiont0

Verbatim

e2

“The type of solid medium tends to shoot induction better than other types of medium”

✓ located verbatim in the source · abstract, characters 722–805 · show in text ↑
t0

“banana shoots (Musa acuminata Colla.) cv. Barangan Merah under in vitro condition”

✓ located verbatim in the source · title, characters 93–174 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Contamination affected 13.9% of the 72 explants planted in banana in vitro culture.

Taxon
bananat0
Outcome
contaminatione3
Value
13.9%e3
Qualifier
of the 72 explants plantede3

Verbatim

e3

“The contamination that occurred was 13.9% of the 72 explants planted”

✓ located verbatim in the source · abstract, characters 807–875 · show in text ↑
t0

“banana shoots (Musa acuminata Colla.) cv. Barangan Merah under in vitro condition”

✓ located verbatim in the source · title, characters 93–174 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Mucor and Aspergillus fungi caused contamination of banana explants in culture.

Taxon
bananat0
Interactor
MucorMucore4other
Second interactor
AspergillusAspergilluse4other
Outcome
contaminatione4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mucor
Mucor · Interactor · picture of the genus (one of its species)James Lindsey · CC BY-SA 3.0 · Wikimedia Commons ↗
Aspergillus
Aspergillus · Second interactor · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e4

“The contamination was caused by Mucor and Aspergillus fungi”

✓ located verbatim in the source · abstract, characters 877–936 · show in text ↑
t0

“banana shoots (Musa acuminata Colla.) cv. Barangan Merah under in vitro condition”

✓ located verbatim in the source · title, characters 93–174 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Gram-positive and gram-negative bacteria (cocci and bacilli) caused contamination of banana explants in culture.

Taxon
bananat0
Interactor
gram positive bacteria (coccus)Coccuse5other
Second interactor
gram-negative bacteria (coccus and bacilli)e5other
Outcome
contaminatione5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Coccus
Coccus · Interactor · picture of the genus (one of its species)Whitney Cranshaw, Colorado State University, United States, Bugwood.org · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e5

“The bacteria causing the contamination were gram positive bacteria (coccus) and gram-negative bacteria (coccus and bacilli)”

✓ located verbatim in the source · abstract, characters 938–1061 · show in text ↑
t0

“banana shoots (Musa acuminata Colla.) cv. Barangan Merah under in vitro condition”

✓ located verbatim in the source · title, characters 93–174 · show in text ↑

in vitro · growth · MusaPositive and negative results

68 papers: 58 positive, 10 negative or no effect

57 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. no effect
  3. negative

64 papers more in this group

See all 68 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.