Paper · 2024 · Journal of Agricultural Sciences – Sri Lanka

In Vitro Effect of PEG and Proline on Callus Growth and Minerals Values in Basmati Rice (Oryza sativa)

S. Adress; M. I. Khokhar; R. N. Fatima; et al.

6
claims
12
verbatim quotes
5
citations
1
research areas
✓
Open access · cc-by-nc-sa

Abstract

Purpose: Drought is a universal issue that disturbs photosynthetic system, adversely affecting quality and crop yield. The study was conducted to determine response of polyethylene glycol (PEG) as stressing agent alone or combination with proline on four fine rice genotypes for callus components as well as biochemical parameters like Chromium (Cr), Magnesium (Mg), Manganese (Mn), Iron (Fe), Phosphorus (P).Research Method: Callus was induced on media having 4.43g/L MS + 4mg/L 2, 4-D (2, 4-dichlorophenoxy acetic acid) + 30g/L sucrose + 1.76g/L phytagel with different levels of PEG “1.25g/L and 2.5g/L” with and without 1ml/L proline.Findings: Callus was used for different analyses after 30 days and results showed that PEG treatments have a negative effect on callus fresh weight, callus dry weight, days to callus induction, callus percentage, and mineral nutrients like K, Cr, Mg, Mn, Fe, P. PEG 2.5g/L showed a greater decrease as compared to PEG 1.25g/L. Proline significantly raises callus growth alone or in combination with PEG but less than control whereas callus dry weight, K, and Ca contents increased more than control when proline applied alone or with PEG combination.Originality/value: Exogenous proline application improves callus growth rate as well as macro- micro elements by mitigating the effect of PEG.

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Claims 6

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

PEG reduced callus fresh and dry weight and callus percentage.

Taxon
Basmati Ricet0
Treatment
PEG treatmentse0
Outcome
callus fresh weight, callus dry weight, days to callus induction, callus percentagee0
Direction
negative effecte0

Not reportedComparator

Verbatim

e0

“PEG treatments have a negative effect on callus fresh weight, callus dry weight, days to callus induction, callus percentage, and mineral nutrients like K, Cr, Mg, Mn, Fe, P.”

✓ located verbatim in the source · abstract, characters 725–899 · show in text ↑
t0

“Basmati Rice (Oryza sativa)”

✓ located verbatim in the source · title, characters 75–102 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

PEG reduced callus mineral nutrients K, Cr, Mg, Mn, Fe and P.

Taxon
Basmati Ricet0
Treatment
PEG treatmentse0
Outcome
mineral nutrients like K, Cr, Mg, Mn, Fe, Pe0
Direction
negative effecte0

Not reportedComparator

Verbatim

e0

“PEG treatments have a negative effect on callus fresh weight, callus dry weight, days to callus induction, callus percentage, and mineral nutrients like K, Cr, Mg, Mn, Fe, P.”

✓ located verbatim in the source · abstract, characters 725–899 · show in text ↑
t0

“Basmati Rice (Oryza sativa)”

✓ located verbatim in the source · title, characters 75–102 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

PEG at 2.5g/L caused a greater decrease than 1.25g/L.

Taxon
Basmati Ricet0
Treatment
PEG 2.5g/Le0
Outcome
decreasee0
Direction
greatere0
Comparator
PEG 1.25g/Le0

Verbatim

e0

“PEG 2.5g/L showed a greater decrease as compared to PEG 1.25g/L.”

✓ located verbatim in the source · abstract, characters 900–964 · show in text ↑
t0

“Basmati Rice (Oryza sativa)”

✓ located verbatim in the source · title, characters 75–102 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Proline alone or with PEG significantly raised callus growth, though less than control.

Taxon
Basmati Ricet0
Treatment
Prolinee0
Outcome
callus growthe0
Direction
raisese0
Qualifier
less than controle0
Comparator
controle0

Verbatim

e0

“Proline significantly raises callus growth alone or in combination with PEG but less than control”

✓ located verbatim in the source · abstract, characters 965–1062 · show in text ↑
t0

“Basmati Rice (Oryza sativa)”

✓ located verbatim in the source · title, characters 75–102 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Proline alone or with PEG increased callus dry weight beyond control.

Taxon
Basmati Ricet0
Treatment
proline applied alone or with PEG combinatione0
Outcome
callus dry weighte0
Direction
increased moree0
Comparator
controle0

Verbatim

e0

“callus dry weight, K, and Ca contents increased more than control when proline applied alone or with PEG combination.”

✓ located verbatim in the source · abstract, characters 1071–1188 · show in text ↑
t0

“Basmati Rice (Oryza sativa)”

✓ located verbatim in the source · title, characters 75–102 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Proline alone or with PEG increased K and Ca contents beyond control.

Taxon
Basmati Ricet0
Treatment
proline applied alone or with PEG combinatione0
Outcome
K, and Ca contentse0
Direction
increased moree0
Comparator
controle0

Verbatim

e0

“callus dry weight, K, and Ca contents increased more than control when proline applied alone or with PEG combination.”

✓ located verbatim in the source · abstract, characters 1071–1188 · show in text ↑
t0

“Basmati Rice (Oryza sativa)”

✓ located verbatim in the source · title, characters 75–102 · show in text ↑

proline · growth · Oryza sativa (rice)Results differ

28 papers: 26 positive, 2 negative or no effect

25 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

24 papers more in this group

See all 28 papers →

poly(ethylene glycol) · nutrient: content · Oryza sativa (rice)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

poly(ethylene glycol) · growth · Oryza sativa (rice)Positive and negative results

29 papers: 9 positive, 20 negative or no effect

8 papers in the same direction as this paper · 19 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

25 papers more in this group

See all 29 papers →

proline · nutrient: content · Oryza sativa (rice)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.