Abstract
While drought resistance is become of increasing importance in rice (Oryza sativa L.), selection under actual field conditions is tedious due to low heritability and time required. Selection in tissue culture is thought to be one way to improve selection efficiency, but this requires standardized protocols. Rice cultivars PAU 201 and PR 116 showed significant callus induction, but the capacity for callus induction and regeneration decreased under polyethylene glycol (PEG) (6000) stress in both cultivars. Calli were induced on semisolid Murahige and Skoog (MS) medium supplemented with 2.5 mg l-1 2, 4-dichlorophenoxy acetic acid (2,4-D) + 0.5 mg l-1 kinetin (kin) + 560 mg l-1 proline + 30 g l-1 sucrose + 8 g l agar-1. Embryogenic calli showed shoot regeneration on MS medium supplemented with 2.0 mg l-1 benzyl aminopurine (BAP) + 0.5 mg l-1 kinetin + 0.5 mg l-1 napthalene acetic acid (NAA) + 30 g l-1 sucrose + 8 g l-1 agar. Increased levels of PEG (6000) (0, 0.5, 1.0, 1.5, 2.0 %) were used to create water stress. There was reduction in callus induction ability and plant regeneration efficiency with increasing levels of PEG (6000) stress. These results indicated that PEG (6000) can be used as water stress creating agent under in vitro conditions and rice variety PR 116 was relatively tolerant to drought stress as compared to PAU 201. This study will serve as a base line for in vitro screening of drought tolerant transgenic rice.
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Claims 5
Claim · model-written summary
Increasing PEG (6000) stress reduced callus induction ability and plant regeneration efficiency in rice.
- Taxon
- rice
Oryza sativat0 - Treatment
- increasing levels of PEG (6000) stresse0
- Outcome
- callus induction ability and plant regeneration efficiencye0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE (ORYZA SATIVA L.)”
e0“There was reduction in callus induction ability and plant regeneration efficiency with increasing levels of PEG (6000) stress”
Claim · model-written summary
Rice variety PR 116 was relatively tolerant to drought stress compared with PAU 201.
- Taxon
- riceOryza sativat0
- Material
- PR 116e1
- Treatment
- drought stresse1
- Outcome
- tolerant to drought stresse1
- Comparator
- PAU 201e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE (ORYZA SATIVA L.)”
e1“rice variety PR 116 was relatively tolerant to drought stress as compared to PAU 201”
Claim · model-written summary
Rice cultivars PAU 201 and PR 116 showed significant callus induction.
- Taxon
- riceOryza sativat0
- Material
- PAU 201 and PR 116e2
- Outcome
- callus inductione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE (ORYZA SATIVA L.)”
e2“Rice cultivars PAU 201 and PR 116 showed significant callus induction”
Claim · model-written summary
Embryogenic calli of rice showed shoot regeneration on MS medium.
- Taxon
- riceOryza sativat0
- Organ / stage
- Embryogenic callie3
- Outcome
- shoot regeneratione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE (ORYZA SATIVA L.)”
e3“Embryogenic calli showed shoot regeneration on MS medium”
Claim · model-written summary
PEG (6000) can serve as a water stress creating agent under in vitro conditions.
- Taxon
- riceOryza sativat0
- Outcome
- water stress creating agent under in vitro conditionse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“RICE (ORYZA SATIVA L.)”
e4“PEG (6000) can be used as water stress creating agent under in vitro conditions”
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See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.