Abstract
Transfer banana plantlet from in vitro to ex vitro conditions is essential prerequisite in micropropagation. In vitro grown plantlets are unable to cope with soil environment. To avoid these challenges, the plant must be acclimatized according to a protocol that takes into account the economics of the plant concerned. Banana shoot culture was established on MS medium supplemented with 5 mg/l BAP and 30 g/l sucrose. Shoot cuttings were subjected for in vitro root formation on half strength MS medium containing 1 mg/l IBA. Plantlets were acclimatized under tissue culture room conditions where they were able to repair their physiological disorders and cope ex vitro conditions. Disorder repair processes were better if plantlets were treated with chitosan. When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase. To save time and costs, root formation and acclimatization were combined in one step under transparent plastic bags (relative humidity 87%) in tissue culture room (25 oC) using ex vitro- hydroponic or soil-root formation procedure. Survival frequency was 100% when root formation was induced using 1 mg/l IBA and relative humidity was gradually decreased. Plantlets were transplanted in soil without application of in vitro or ex vitro root formation procedures died in five days.
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Claims 8
Claim · model-written summary
Chitosan treatment gave better disorder repair in acclimatizing banana plantlets.
Not reportedComparator
Verbatim
t0“Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions”
e2“Disorder repair processes were better if plantlets were treated with chitosan”
Claim · model-written summary
Chitosan increased the efficiency of acclimatization during all acclimatization periods.
Not reportedComparator
Verbatim
t0“Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions”
e1“When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase”
Claim · model-written summary
Chitosan increased plant growth parameters in banana plantlets.
Not reportedComparator
Verbatim
t0“Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions”
e1“When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase”
Claim · model-written summary
Chitosan increased water content in banana plantlets.
Not reportedComparator
Verbatim
t0“Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions”
e1“When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase”
Claim · model-written summary
Chitosan increased ROS scavenging in banana plantlets.
Not reportedComparator
Verbatim
t0“Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions”
e1“When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase”
Claim · model-written summary
Chitosan increased the activities of antioxidant enzymes, superoxide dismutase and peroxidase, in banana plantlets.
- Taxon
- bananat0
- Treatment
- chitosane1
- Gene
- superoxide dismutase and peroxidasee1
- Outcome
- activities of antioxidant enzymese1
- Direction
- increasee1
Not reportedComparator
Verbatim
t0“Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions”
e1“When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase”
Claim · model-written summary
Survival frequency was 100% when root formation used 1 mg/l IBA and relative humidity was gradually decreased.
Verbatim
t0“Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions”
e3“Survival frequency was 100% when root formation was induced using 1 mg/l IBA and relative humidity was gradually decreased”
Claim · model-written summary
Plantlets transplanted into soil without in vitro or ex vitro root formation procedures died within five days.
- Taxon
- bananat0
- Treatment
- transplanted in soil without application of in vitro or ex vitro root formation procedurese4
- Outcome
- diede4
- Qualifier
- in five dayse4
Not reportedComparatorDirection
Verbatim
t0“Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions”
e4“Plantlets were transplanted in soil without application of in vitro or ex vitro root formation procedures died in five days”
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