Abstract
There is an increasing trend in forest production towards planting rapid-growing trees as attractive, environmentally friendly energy sources. This study aimed to establish an alternative to the traditional propagation of a number of selections of Robinia pseudoacacia L. by developing an in vitro culture protocol. This study’s topic is of great importance, and it reflects an ongoing concern at the University of Oradea’s Faculty of Environmental Protection’s sustainable research program. The explants from four forms (called S1, S2, S3, and S4), selected for their phenotypic characteristics, were inoculated on four culture media (Murashige–Skoog (MS), Anderson, Chée–Pool, and Driver and Kuniyuki Woody (DKW)) with the same phytohormonal balance. DKW medium proved to be the better support of morphogenic activity, and it was further tested under different phytohormonal balances. Different results were observed depending on the hormone content in the DKW environment. In the presence of 0.5 mg/L benzylaminopurine (BAP) and 0.04 mg/L aminoisobutyric acid (AIB), 91.5% of the explants developed an average of 4.45 ± 0.18 shoots, whereas the average upper shoot height (3.82 cm) was recorded on DKW medium with 0.5 mg/L BAP and 0.04 mg/L α-naphthaleneacetic acid (NAA). Auxin, 0.05 mg/L AIB, promoted root production (5.27 ± 0.15 roots/explant), while 0.1 mg/L NAA promoted root length. In conclusion, the S4 selection produced the greatest outcomes of all environmental variables in terms of both the number of shoots and their heights.
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Claims 7
Claim · model-written summary
DKW medium best supported morphogenic activity.
- Taxon
- Robinia pseudoacacia L.
Robinia pseudoacaciat0 - Treatment
- DKW mediume0
- Outcome
- morphogenic activitye0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DKW medium proved to be the better support of morphogenic activity”
t0“Robinia pseudoacacia L.”
Claim · model-written summary
DKW with BAP and AIB produced shoots on 91.5% of explants.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- 0.5 mg/L benzylaminopurine (BAP) and 0.04 mg/L aminoisobutyric acid (AIB)e0
- Outcome
- explants developede0
- Direction
- increaseinferrede0
- Value
- 91.5%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of 0.5 mg/L benzylaminopurine (BAP) and 0.04 mg/L aminoisobutyric acid (AIB), 91.5% of the explants developed an average of 4.45 ± 0.18 shoots”
t0“Robinia pseudoacacia L.”
Claim · model-written summary
That BAP and AIB combination yielded 4.45 ± 0.18 shoots on average.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- 0.5 mg/L benzylaminopurine (BAP) and 0.04 mg/L aminoisobutyric acid (AIB)e0
- Outcome
- shootse0
- Direction
- increaseinferrede0
- Value
- 4.45 ± 0.18 shootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of 0.5 mg/L benzylaminopurine (BAP) and 0.04 mg/L aminoisobutyric acid (AIB), 91.5% of the explants developed an average of 4.45 ± 0.18 shoots”
t0“Robinia pseudoacacia L.”
Claim · model-written summary
DKW with BAP and NAA produced an upper shoot height of 3.82 cm.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- DKW medium with 0.5 mg/L BAP and 0.04 mg/L α-naphthaleneacetic acid (NAA)e0
- Outcome
- average upper shoot heighte0
- Direction
- higherinferrede0
- Value
- 3.82 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“average upper shoot height (3.82 cm) was recorded on DKW medium with 0.5 mg/L BAP and 0.04 mg/L α-naphthaleneacetic acid (NAA)”
t0“Robinia pseudoacacia L.”
Claim · model-written summary
AIB at 0.05 mg/L promoted 5.27 ± 0.15 roots per explant.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- 0.05 mg/L AIBe0
- Outcome
- root productione0
- Direction
- promotede0
- Value
- 5.27 ± 0.15 roots/explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.05 mg/L AIB, promoted root production (5.27 ± 0.15 roots/explant)”
t0“Robinia pseudoacacia L.”
Claim · model-written summary
NAA at 0.1 mg/L promoted root length.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Treatment
- 0.1 mg/L NAAe0
- Outcome
- root lengthe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.1 mg/L NAA promoted root length”
t0“Robinia pseudoacacia L.”
Claim · model-written summary
Selection S4 had the greatest shoot number and height responses.
- Taxon
- Robinia pseudoacacia L.Robinia pseudoacaciat0
- Material
- S4 selectione0
- Outcome
- number of shoots and their heightse0
- Direction
- greateste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the S4 selection produced the greatest outcomes of all environmental variables in terms of both the number of shoots and their heights”
t0“Robinia pseudoacacia L.”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.