Paper · 2000 · Acta Horticulturae

MICRO-ORGANISMS ACTING IN TISSUE CULTURES OF BLACK LOCUST (ROBINIA PSEUDOACACIA L.)

G. Naujoks; I. Zaspel; U. Behrendt

6
claims
10
verbatim quotes
7
citations
3
research areas
—
Not open access

Abstract

In the course of developing an appropriate micropropagation method for selected, straight growing genotypes of black locust (Robinia pseudoacacia L.) the enrichment of endogenous bacteria in shoot cultures was observed. It was not possible to suppress the unwanted micro-organisms by adding the antimicrobial stain Malachite Green to the nutrient medium. The bacteria were identified as two strains of Curtobacterium sp. A detrimental effect of the culture filtrate of both strains on callus and shoot cultures was not evident. The survival and growth of micropropagated black locust plantlets during transfer to soil was improved by an early inoculation of rooted shoots in vitro with different nodule forming bacteria strains isolated from older black locust trees.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Endogenous bacteria became enriched in black locust shoot cultures.

Taxon
BLACK LOCUSTRobinia pseudoacaciat0
Outcome
enrichment of endogenous bacteria in shoot culturese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the enrichment of endogenous bacteria in shoot cultures was observed”

✓ located verbatim in the source · abstract, characters 150–218 · show in text ↑
t0

“BLACK LOCUST (ROBINIA PSEUDOACACIA L.)”

✓ located verbatim in the source · title, characters 45–83 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Malachite Green did not suppress the unwanted microorganisms in the culture medium.

Taxon
BLACK LOCUSTRobinia pseudoacaciat0
Treatment
Malachite Greene0
Outcome
unwanted micro-organismse0
Direction
not possible to suppresse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“It was not possible to suppress the unwanted micro-organisms by adding the antimicrobial stain Malachite Green to the nutrient medium”

✓ located verbatim in the source · abstract, characters 220–353 · show in text ↑
t0

“BLACK LOCUST (ROBINIA PSEUDOACACIA L.)”

✓ located verbatim in the source · title, characters 45–83 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The associated bacteria were identified as two Curtobacterium sp. strains.

Taxon
Curtobacterium sp.Curtobacteriume0
Outcome
two strains of Curtobacterium sp.e0

Verbatim

e0

“The bacteria were identified as two strains of Curtobacterium sp.”

✓ located verbatim in the source · abstract, characters 355–420 · show in text ↑
t0

“BLACK LOCUST (ROBINIA PSEUDOACACIA L.)”

✓ located verbatim in the source · title, characters 45–83 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Filtrates of both bacterial strains showed no evident detrimental effect on callus and shoot cultures.

Taxon
BLACK LOCUSTRobinia pseudoacaciat0
Organ / stage
callus and shoot culturese0
Treatment
culture filtrate of both strainse0
Outcome
detrimental effecte0
Direction
not evidente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A detrimental effect of the culture filtrate of both strains on callus and shoot cultures was not evident”

✓ located verbatim in the source · abstract, characters 421–526 · show in text ↑
t0

“BLACK LOCUST (ROBINIA PSEUDOACACIA L.)”

✓ located verbatim in the source · title, characters 45–83 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Early inoculation of rooted shoots with nodule forming bacteria improved plantlet survival during soil transfer.

Taxon
black locustRobinia pseudoacaciae0
Treatment
early inoculation of rooted shoots in vitro with different nodule forming bacteria strainse0
Interactor
nodule forming bacteria strainse0symbiont
Outcome
survivale0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The survival and growth of micropropagated black locust plantlets during transfer to soil was improved by an early inoculation of rooted shoots in vitro with different nodule forming bacteria strains isolated from older black locust trees”

✓ located verbatim in the source · abstract, characters 528–766 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Early inoculation of rooted shoots with nodule forming bacteria improved plantlet growth during soil transfer.

Taxon
black locustRobinia pseudoacaciae0
Treatment
early inoculation of rooted shoots in vitro with different nodule forming bacteria strainse0
Interactor
nodule forming bacteria strainse0symbiont
Outcome
growthe0
Direction
improvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The survival and growth of micropropagated black locust plantlets during transfer to soil was improved by an early inoculation of rooted shoots in vitro with different nodule forming bacteria strains isolated from older black locust trees”

✓ located verbatim in the source · abstract, characters 528–766 · show in text ↑

in vitro · survival · Robinia pseudoacacia (black locust)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

in vitro · growth · Robinia pseudoacacia (black locust)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

inoculation · growth · Robinia pseudoacacia (black locust)Results agree

11 papers: 11 positive, 0 negative or no effect

10 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.