Abstract
Robinia pseudoacacia var. umbraculifera, commonly called umbrella black locust were regenerated after co-cultivation of internode segments with Agrobacterium tumefaciens which included yeast cadmium factor 1 (YCF 1) gene. The tolerance to cadmium and lead for plants can be increased by the YCF1 gene expression. Moreover, the recent studies have shown that YCF1 gene transgenic plants increase the accumulation of cadmium and lead into plant vacuoles. The effect of plant growth regulator such as 2,4-dichlorophenoxyacetic acid (2,4-D), ${\alpha}$-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and thidiazuron (TDZ) were studied to evaluate the propagation of plants through internode explants. The efficient induction of multiple adventitious shoots and callus were observed on a medium supplemented with 0.1 mg/L TDZ + 0.2 mg/L BA. To induce shoot elongation and rooting, regenerated shoots were transferred into basal MS medium without any plant growth regulator. Successful Agrobacterium tumefaciens mediated transformation was obtained by 20 min vacuum-infiltration with $50{\mu}M$ acetosyringone on the optimal multiple shoot induction medium with 30 mg/L hygromycin and 300 mg/L cefotaxime. To confirm the integration and expression of transgene, Polymerase Chain Reaction (PCR) and Reverse Transcriptase PCR (RT-PCR) were performed with specific primers. The frequency of transformation was approximately 18.94%. This study can be used to genetic engineering of phytoremediator.
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Claims 3
Claim · model-written summary
TDZ plus BA medium efficiently induced multiple adventitious shoots and callus.
- Taxon
- Robinia pseudoacacia var. umbraculifera
Robinia pseudoacaciat0 - Treatment
- 0.1 mg/L TDZ + 0.2 mg/L BAe0
- Outcome
- multiple adventitious shoots and calluse0
- Direction
- efficient inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The efficient induction of multiple adventitious shoots and callus were observed on a medium supplemented with 0.1 mg/L TDZ + 0.2 mg/L BA.”
t0“Robinia pseudoacacia var. umbraculifera”
Claim · model-written summary
Vacuum infiltration with acetosyringone yielded successful Agrobacterium-mediated transformation.
- Taxon
- Robinia pseudoacacia var. umbraculiferaRobinia pseudoacaciat0
- Treatment
- 20 min vacuum-infiltration with $50{\mu}M$ acetosyringonee0
- Interactor
- Agrobacterium tumefaciens
Agrobacterium tumefaciense0other - Outcome
- Successful Agrobacterium tumefaciens mediated transformatione0
- Value
- 20 mine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successful Agrobacterium tumefaciens mediated transformation was obtained by 20 min vacuum-infiltration with $50{\mu}M$ acetosyringone on the optimal multiple shoot induction medium with 30 mg/L hygromycin and 300 mg/L cefotaxime.”
t0“Robinia pseudoacacia var. umbraculifera”
Claim · model-written summary
Transformation frequency was approximately 18.94%.
- Taxon
- Robinia pseudoacacia var. umbraculiferaRobinia pseudoacaciat0
- Outcome
- frequency of transformatione0
- Value
- 18.94%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The frequency of transformation was approximately 18.94%.”
t0“Robinia pseudoacacia var. umbraculifera”
The same questions in other papers
Thidiazuron · growth · Robinia pseudoacacia (black locust)Results agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · growth · Robinia pseudoacacia (black locust)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.