Abstract
Bacterial blight seriously affects the growth and production of cassava (Manihot esculenta Crantz), but disease resistance genes and the underlying molecular mechanism remain unknown. In this study, we found that LESION SIMULATING DISEASE 3 (MeLSD3) is essential for disease resistance in cassava. MeLSD3 physically interacts with SIRTUIN 1 (MeSRT1), inhibiting MeSRT1-mediated deacetylation modification at the acetylation of histone 3 at K9 (H3K9Ac). This leads to increased H3K9Ac levels and transcriptional activation of SUPPRESSOR OF BIR1 (SOBIR1) and FLAGELLIN-SENSITIVE2 (FLS2) in pattern-triggered immunity, resulting in immune responses in cassava. When MeLSD3 was silenced, the release of MeSRT1 directly decreased H3K9Ac levels and inhibited the transcription of SOBIR1 and FLS2, leading to decreased disease resistance. Notably, DELLA protein GIBBERELLIC ACID INSENSITIVE 1 (MeGAI1) also interacted with MeLSD3, which enhanced the interaction between MeLSD3 and MeSRT1 and further strengthened the inhibition of MeSRT1-mediated deacetylation modification at H3K9Ac of defense genes. In summary, this study illustrates the mechanism by which MeLSD3 interacts with MeSRT1 and MeGAI1, thereby mediating the level of H3K9Ac and the transcription of defense genes and immune responses in cassava.
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Claims 7
Claim · model-written summary
MeLSD3 is essential for disease resistance in cassava.
- Taxon
- cassava
Manihot esculentae1 - Gene
- LESION SIMULATING DISEASE 3 (MeLSD3)e1
- Outcome
- disease resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“LESION SIMULATING DISEASE 3 (MeLSD3) is essential for disease resistance in cassava”
Claim · model-written summary
MeLSD3 interacts with MeSRT1, inhibiting its deacetylation of H3K9Ac, which increases H3K9Ac levels.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeSRT1e2
- Outcome
- H3K9Ac levelse3
- Direction
- increasede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cassava”
e2“MeLSD3 physically interacts with SIRTUIN 1 (MeSRT1), inhibiting MeSRT1-mediated deacetylation modification at the acetylation of histone 3 at K9 (H3K9Ac)”
e3“This leads to increased H3K9Ac levels”
Claim · model-written summary
Silencing MeLSD3 decreased disease resistance in cassava.
- Taxon
- cassavaManihot esculentat0
- Treatment
- When MeLSD3 was silencede4
- Outcome
- disease resistancee4
- Direction
- decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cassava”
e4“When MeLSD3 was silenced, the release of MeSRT1 directly decreased H3K9Ac levels and inhibited the transcription of SOBIR1 and FLS2, leading to decreased disease resistance”
Claim · model-written summary
Silencing MeLSD3 decreased H3K9Ac levels in cassava.
- Taxon
- cassavaManihot esculentat0
- Treatment
- When MeLSD3 was silencede4
- Outcome
- H3K9Ac levelse4
- Direction
- decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cassava”
e4“When MeLSD3 was silenced, the release of MeSRT1 directly decreased H3K9Ac levels and inhibited the transcription of SOBIR1 and FLS2, leading to decreased disease resistance”
Claim · model-written summary
Silencing MeLSD3 inhibited transcription of SOBIR1 and FLS2 in cassava.
- Taxon
- cassavaManihot esculentat0
- Treatment
- When MeLSD3 was silencede4
- Gene
- SOBIR1 and FLS2e4
- Outcome
- transcription of SOBIR1 and FLS2e4
- Direction
- inhibitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cassava”
e4“When MeLSD3 was silenced, the release of MeSRT1 directly decreased H3K9Ac levels and inhibited the transcription of SOBIR1 and FLS2, leading to decreased disease resistance”
Claim · model-written summary
MeGAI1 interacts with MeLSD3 and enhances the interaction between MeLSD3 and MeSRT1 in cassava.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeGAI1e5
- Outcome
- interaction between MeLSD3 and MeSRT1e5
- Direction
- enhancede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cassava”
e5“Notably, DELLA protein GIBBERELLIC ACID INSENSITIVE 1 (MeGAI1) also interacted with MeLSD3, which enhanced the interaction between MeLSD3 and MeSRT1”
Claim · model-written summary
MeGAI1 further strengthens the inhibition of MeSRT1-mediated deacetylation at H3K9Ac in cassava.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeGAI1e5
- Outcome
- inhibition of MeSRT1-mediated deacetylation modification at H3K9Ace6
- Direction
- strengthenede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cassava”
e5“Notably, DELLA protein GIBBERELLIC ACID INSENSITIVE 1 (MeGAI1) also interacted with MeLSD3, which enhanced the interaction between MeLSD3 and MeSRT1”
e6“further strengthened the inhibition of MeSRT1-mediated deacetylation modification at H3K9Ac of defense genes”
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.