Abstract
Agrobacterium-mediated transient expression and virus-induced gene silencing (VIGS) are very useful in functional genomics in plants. However, whether these methods are effective in cassava (Manihot esculenta), one of the most important tropical crops, remains elusive. In this study, we used green fluorescent protein (GFP) and β-glucuronidase (GUS) as reporter genes in a transient expression assay. GFP or GUS could be detected in the infiltrated leaves at 2 days postinfiltration (dpi) and were evidenced by visual GFP and GUS assays, reverse-transcription PCR, and Western blot. In addition, phytoene desaturase (PDS) was used to show the silencing effect in a VIGS system. Both Agrobacterium GV3101 and AGL-1 with tobacco rattle virus (TRV)-MePDS-infiltrated distal leaves showed an albino phenotype at 20 dpi; in particular, the AGL-1-infiltrated plants showed an obvious albino area in the most distal leaves. Moreover, the silencing effect was validated by molecular identification. Notably, compared with the obvious cassava mosaic disease symptom infiltrated by African-cassava-mosaic-virus-based VIGS systems in previous studies, TRV-based VIGS-system-infiltrated cassava plants did not show obvious virus-induced disease symptoms, suggesting a significant advantage. Taken together, these methods could promote functional genomics in cassava.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
GFP or GUS reporter expression could be detected in cassava infiltrated leaves at 2 days postinfiltration.
- Taxon
- cassava
Manihot esculentae0 - Outcome
- GFP or GUS could be detectede1
- Value
- 2 days postinfiltration (dpi)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effective in cassava (Manihot esculenta)”
e1“GFP or GUS could be detected in the infiltrated leaves at 2 days postinfiltration (dpi)”
Claim · model-written summary
Both Agrobacterium GV3101 and AGL-1 carrying TRV-MePDS showed an albino phenotype in distal cassava leaves at 20 dpi.
- Taxon
- cassavaManihot esculentae0
- Outcome
- albino phenotypee2
- Value
- 20 dpie2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effective in cassava (Manihot esculenta)”
e2“Both Agrobacterium GV3101 and AGL-1 with tobacco rattle virus (TRV)-MePDS-infiltrated distal leaves showed an albino phenotype at 20 dpi”
Claim · model-written summary
AGL-1-infiltrated cassava plants showed an obvious albino area in the most distal leaves.
- Taxon
- cassavaManihot esculentae0
- Outcome
- albino area in the most distal leavese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effective in cassava (Manihot esculenta)”
e3“in particular, the AGL-1-infiltrated plants showed an obvious albino area in the most distal leaves”
Claim · model-written summary
The silencing effect in cassava was validated by molecular identification.
- Taxon
- cassavaManihot esculentae0
- Outcome
- silencing effecte4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effective in cassava (Manihot esculenta)”
e4“Moreover, the silencing effect was validated by molecular identification”
Claim · model-written summary
TRV-based VIGS-infiltrated cassava plants showed no obvious virus-induced disease symptoms, unlike the obvious cassava mosaic symptoms of African-cassava-mosaic-virus-based VIGS in previous studies.
- Taxon
- cassavaManihot esculentae5
- Treatment
- TRV-based VIGS-systeme5
- Outcome
- virus-induced disease symptomse5
- Direction
- did not showe5
- Comparator
- African-cassava-mosaic-virus-based VIGS systemse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“TRV-based VIGS-system-infiltrated cassava plants did not show obvious virus-induced disease symptoms”
e6“compared with the obvious cassava mosaic disease symptom infiltrated by African-cassava-mosaic-virus-based VIGS systems in previous studies”
The same questions in other papers
loss of function · disease · Manihot esculenta (cassava)Positive and negative results
5 papers in the same direction as this paper · 5 in the opposite direction
- negative
- positive
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.