Abstract
BACKGROUND AND AIMS: The nuclear factor Y (NF-Y) transcription factor complex is important in plant growth, development and stress response. Information regarding this transcription factor complex is limited in cassava (Manihot esculenta). In this study, 15 MeNF-YAs, 21 MeNF-YBs and 15 MeNF-YCs were comprehensively characterized during plant defence. METHODS: Gene expression in MeNF-Ys was examined during interaction with the bacterial pathogen Xanthomonas axonopodis pv. manihotis (Xam). The yeast two-hybrid system was employed to investigate protein-protein interactions in the heterotrimeric NF-Y transcription factor complex. The in vivo roles of MeNF-Ys were revealed by virus-induced gene silencing (VIGS) in cassava. KEY RESULTS: The regulation of MeNF-Ys in response to Xam indicated their possible roles in response to cassava bacterial blight. Protein-protein interaction assays identified the heterotrimeric NF-Y transcription factor complex (MeNF-YA1/3, MeNF-YB11/16 and MeNF-YC11/12). Moreover, the members of the heterotrimeric NF-Y transcription factor complex were located in the cell nucleus and conferred transcriptional activation activity to the CCAAT motif. Notably, the heterotrimeric NF-Y transcription factor complex positively regulated plant disease resistance to Xam, confirmed by a disease phenotype in overexpressing plants in Nicotiana benthamiana and VIGS in cassava. Consistently, the heterotrimeric NF-Y transcription factor complex positively regulated the expression of pathogenesis-related genes (MePRs). CONCLUSIONS: The NF-Y transcription factor complex (MeNF-YA1/3, MeNF-YB11/16 and MeNF-YC11/12) characterized here was shown to play a role in transcriptional activation of MePR promoters, contributing to the plant defence response in cassava.
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Claims 6
Claim · model-written summary
MeNF-Y genes were regulated in response to Xam.
- Taxon
- cassava
Manihot esculentae0 - Interactor
- Xame0pathogen
- Gene
- MeNF-Yse0
- Outcome
- regulation of MeNF-Yse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regulation of MeNF-Ys in response to Xam indicated their possible roles in response to cassava bacterial blight”
Claim · model-written summary
Protein interaction assays identified a heterotrimeric NF-Y complex.
- Taxon
- cassavaManihot esculentat0
- Gene
- MeNF-YA1/3, MeNF-YB11/16 and MeNF-YC11/12e0
- Outcome
- heterotrimeric NF-Y transcription factor complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein-protein interaction assays identified the heterotrimeric NF-Y transcription factor complex (MeNF-YA1/3, MeNF-YB11/16 and MeNF-YC11/12)”
t0“cassava”
Claim · model-written summary
The complex members were located in the cell nucleus.
- Taxon
- cassavaManihot esculentat0
- Gene
- heterotrimeric NF-Y transcription factor complexe0
- Outcome
- located in the cell nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the members of the heterotrimeric NF-Y transcription factor complex were located in the cell nucleus”
t0“cassava”
Claim · model-written summary
The complex conferred transcriptional activation activity to the CCAAT motif.
- Taxon
- cassavaManihot esculentat0
- Gene
- CCAAT motife0
- Outcome
- transcriptional activation activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“conferred transcriptional activation activity to the CCAAT motif”
t0“cassava”
Claim · model-written summary
The complex positively regulated resistance to Xam in plant disease assays.
- Taxon
- cassavaManihot esculentae0
- Treatment
- VIGS in cassavae0
- Interactor
- Xame0pathogen
- Outcome
- plant disease resistance to Xame0
- Direction
- positively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heterotrimeric NF-Y transcription factor complex positively regulated plant disease resistance to Xam, confirmed by a disease phenotype in overexpressing plants in Nicotiana benthamiana and VIGS in cassava”
Claim · model-written summary
The complex positively regulated pathogenesis-related gene expression.
- Taxon
- cassavaManihot esculentat0
- Gene
- MePRse0
- Outcome
- expression of pathogenesis-related genese0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heterotrimeric NF-Y transcription factor complex positively regulated the expression of pathogenesis-related genes (MePRs)”
t0“cassava”
The same questions in other papers
loss of function · disease · Manihot esculenta (cassava)Positive and negative results
5 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.