Abstract
Tomato (Solanum lycopersicum), an economically important vegetable crop cultivated worldwide, often suffers massive financial losses due to Phytophthora infestans (P. infestans) spread and breakouts. Arbuscular mycorrhiza (AM) fungi mediated biocontrol has demonstrated great potential in plant resistance. However, little information is available on the regulation of mycorrhizal tomato resistance against P. infestans. Therefore, microRNAs (miRNAs) sequencing technology was used to analyse miRNA and their targets in the mycorrhizal tomato after P. infestans infection. Our study showed a lower severity of necrotic lesions in mycorrhizal tomato than in nonmycorrhizal controls. We investigated 35 miRNAs that showed the opposite expression tendency in mycorrhizal and nonmycorrhizal tomato after P. infestans infection when compared with uninfected P. infestans. Among them, miR319c was upregulated in mycorrhizal tomato leaves after pathogen infection. Overexpression of miR319c or silencing of its target gene (TCP1) increased tomato resistance to P. infestans, implying that miR319c acts as a positive regulator in tomato after pathogen infection. Additionally, we examined the induced expression patterns of miR319c and TCP1 in tomato plants exposed to salicylic acid (SA) treatment, and SA content and the expression levels of SA-related genes were also measured in overexpressing transgenic plants. The result revealed that miR319c can not only participates in tomato resistance to P. infestans by regulating SA content, but also indirectly regulates the expression levels of key genes in the SA pathway by regulating TCP1.In this study, we propose a novel mechanism in which the miR319c in mycorrhizal tomato increases resistance to P. infestans.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Mycorrhizal tomato showed lower necrotic lesion severity than nonmycorrhizal controls after P. infestans infection
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- mycorrhizale1
- Interactor
- Phytophthora infestans
Phytophthora infestanse0pathogen - Outcome
- severity of necrotic lesionse1
- Direction
- lowere1
- Comparator
- nonmycorrhizal controlse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“often suffers massive financial losses due to Phytophthora infestans (P. infestans) spread and breakouts”
e1“Our study showed a lower severity of necrotic lesions in mycorrhizal tomato than in nonmycorrhizal controls”
Claim · model-written summary
Thirty-five miRNAs showed opposite expression tendencies in mycorrhizal versus nonmycorrhizal tomato after P. infestans infection
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Outcome
- opposite expression tendencye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We investigated 35 miRNAs that showed the opposite expression tendency in mycorrhizal and nonmycorrhizal tomato after P. infestans infection when compared with uninfected P. infestans”
Claim · model-written summary
miR319c was upregulated in mycorrhizal tomato leaves after pathogen infection
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- leavese1
- Gene
- miR319ce1
- Outcome
- miR319ce1
- Direction
- upregulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Among them, miR319c was upregulated in mycorrhizal tomato leaves after pathogen infection”
Claim · model-written summary
Overexpression of miR319c increased tomato resistance to P. infestans
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Overexpression of miR319ce1
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- miR319ce1
- Outcome
- tomato resistance to P. infestanse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Overexpression of miR319c or silencing of its target gene (TCP1) increased tomato resistance to P. infestans”
Claim · model-written summary
Silencing of the target gene TCP1 increased tomato resistance to P. infestans
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- silencing of its target gene (TCP1)e1
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- TCP1e1
- Outcome
- tomato resistance to P. infestanse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Overexpression of miR319c or silencing of its target gene (TCP1) increased tomato resistance to P. infestans”
Claim · model-written summary
Expression patterns of miR319c and TCP1 were induced in tomato plants exposed to salicylic acid treatment
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- salicylic acid (SA) treatmente1
- Gene
- TCP1e1
- Outcome
- expression patterns of miR319c and TCP1e1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we examined the induced expression patterns of miR319c and TCP1 in tomato plants exposed to salicylic acid (SA) treatment”
Claim · model-written summary
miR319c contributes to tomato resistance to P. infestans by regulating salicylic acid content
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- miR319ce1
- Compound
- SAe1
- Outcome
- tomato resistance to P. infestanse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The result revealed that miR319c can not only participates in tomato resistance to P. infestans by regulating SA content”
Claim · model-written summary
miR319c indirectly regulates expression levels of key SA-pathway genes by regulating TCP1
- Taxon
- TomatoSolanum lycopersicume0
- Gene
- TCP1e1
- Outcome
- expression levels of key genes in the SA pathwaye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato (Solanum lycopersicum), an economically important vegetable crop”
e1“but also indirectly regulates the expression levels of key genes in the SA pathway by regulating TCP1”
The same questions in other papers
loss of function · Phytophthora infestans · Solanum lycopersicum (tomato)Positive and negative results
9 papers in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.