Abstract
Tomato is an important horticultural and economic crop cultivated worldwide. As Phytophthora infestans becomes a huge threat to tomato production, it is necessary to study the resistance mechanisms of tomato against P. infestans. Our previous research has found that miR482 might be involved in tomato–P. infestans interaction. In this study, miR482b precursor was cloned from Solanum pimpinellifolium “L3708” and miR482b was shown to decrease in abundance in tomato following P. infestans infection. Compared to wild-type tomato plants, tomato plants that overexpressed miR482b displayed more serious disease symptoms after P. infestans infection, with more necrotic cells, longer lesion diameters, and increased P. infestans abundance. Meanwhile, silencing of miR482b was performed by short tandem target mimic (STTM), resulting in enhancement of tomato resistance to P. infestans. Using miRNA and degradome data sets, NBS–LRR disease-resistance genes targeted by miR482b were validated. Negative correlation between the expression of miR482b and its target genes was found in all miR482b-overexpressing and -silencing tomato plants. Our results provide insight into tomato miR482b involved in the response to P. infestans infection, and demonstrate that miR482b–NBS–LRR is an important component in the network of tomato–P. infestans interaction.
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Claims 8
Claim · model-written summary
Tomato miR482b abundance decreased after P. infestans infection.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- P. infestans infectione0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Gene
- miR482be0
- Outcome
- miR482be0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR482b was shown to decrease in abundance in tomato following P. infestans infection”
Claim · model-written summary
miR482b overexpression caused more serious disease symptoms than wild type after infection.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- overexpressed miR482be0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- miR482be0
- Outcome
- disease symptomse0
- Direction
- more seriouse0
- Comparator
- wild-type tomato plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to wild-type tomato plants, tomato plants that overexpressed miR482b displayed more serious disease symptoms after P. infestans infection, with more necrotic cells, longer lesion diameters, and increased P. infestans abundance”
Claim · model-written summary
miR482b overexpression increased necrotic cells after infection.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- overexpressed miR482be0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- miR482be0
- Outcome
- necrotic cellse0
- Direction
- moree0
- Comparator
- wild-type tomato plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to wild-type tomato plants, tomato plants that overexpressed miR482b displayed more serious disease symptoms after P. infestans infection, with more necrotic cells, longer lesion diameters, and increased P. infestans abundance”
Claim · model-written summary
miR482b overexpression increased lesion diameter after infection.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- overexpressed miR482be0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- miR482be0
- Outcome
- lesion diameterse0
- Direction
- longere0
- Comparator
- wild-type tomato plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to wild-type tomato plants, tomato plants that overexpressed miR482b displayed more serious disease symptoms after P. infestans infection, with more necrotic cells, longer lesion diameters, and increased P. infestans abundance”
Claim · model-written summary
miR482b overexpression increased pathogen abundance after infection.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- overexpressed miR482be0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- miR482be0
- Outcome
- P. infestans abundancee0
- Direction
- increasede0
- Comparator
- wild-type tomato plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to wild-type tomato plants, tomato plants that overexpressed miR482b displayed more serious disease symptoms after P. infestans infection, with more necrotic cells, longer lesion diameters, and increased P. infestans abundance”
Claim · model-written summary
Silencing miR482b enhanced tomato resistance to P. infestans.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencing of miR482be0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- miR482be0
- Outcome
- tomato resistance to P. infestanse0
- Direction
- enhancemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of miR482b was performed by short tandem target mimic (STTM), resulting in enhancement of tomato resistance to P. infestans”
Claim · model-written summary
NBS–LRR resistance genes targeted by miR482b were validated.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- NBS–LRR disease-resistance genese0
- Outcome
- targeted by miR482be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NBS–LRR disease-resistance genes targeted by miR482b were validated”
t0“tomato resistance”
Claim · model-written summary
miR482b and target gene expression were negatively correlated in overexpressing and silenced plants.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- miR482be0
- Outcome
- expression of miR482b and its target genese0
- Direction
- Negative correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Negative correlation between the expression of miR482b and its target genes was found in all miR482b-overexpressing and -silencing tomato plants”
The same questions in other papers
loss of function · Phytophthora infestans · Solanum lycopersicum (tomato)Positive and negative results
9 papers in the same direction as this paper · 2 in the opposite direction
- negative
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.