Abstract
The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans. The long non-coding RNA (lncRNA) Sl-lncRNA39896 is induced after P. infestans infection and was previously predicted to act as an endogenous target mimic (eTM) for the microRNA Sl-miR166b, which function in stress responses. Here, we further examined the role of Sl-lncRNA39896 and Sl-miR166b in tomato resistance to P. infestans. Sl-miR166b levels were higher in Sl-lncRNA39896-knockout mutants than in wild-type plants, and the mutants displayed enhanced resistance to P. infestans. A six-point mutation in the region of Sl-lncRNA39896 that binds to Sl-miR166b disabled the interaction, suggesting that Sl-lncRNA39896 acts as an eTM for Sl-miR166b. Overexpressing Sl-miR166b yielded a similar phenotype to that produced by Sl-lncRNA39896-knockout, whereas silencing of Sl-miR166b impaired resistance. We verified that Sl-miR166b cleaved transcripts of its target class III homeodomain-leucine zipper genes SlHDZ34 and SlHDZ45. Silencing of SlHDZ34/45 decreased pathogen accumulation in plants infected with P. infestans. Additionally, jasmonic acid and ethylene contents were elevated following infection in the plants with enhanced resistance. Sl-lncRNA39896 is the first known lncRNA to negatively regulate resistance to P. infestans in tomato. We propose a novel mechanism in which the lncRNA39896-miR166b-HDZ module modulates resistance to P. infestans.
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Claims 11
Claim · model-written summary
Sl-miR166b levels were higher in Sl-lncRNA39896-knockout mutants than in wild-type tomato plants.
- Taxon
- tomato
Solanum lycopersicume0 - Material
- Sl-lncRNA39896-knockout mutantse1
- Treatment
- Sl-lncRNA39896-knockoute1
- Gene
- Sl-miR166be1
- Outcome
- Sl-miR166b levelse1
- Direction
- highere1
- Comparator
- wild-type plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e1“Sl-miR166b levels were higher in Sl-lncRNA39896-knockout mutants than in wild-type plants”
Claim · model-written summary
Sl-lncRNA39896-knockout mutants displayed enhanced resistance to P. infestans compared with wild-type tomato plants.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- Sl-lncRNA39896-knockout mutantse1
- Treatment
- Sl-lncRNA39896-knockoute1
- Interactor
- P. infestans
Phytophthora infestanse2pathogen - Outcome
- resistancee2
- Direction
- enhancede2
- Comparator
- wild-type plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e1“Sl-miR166b levels were higher in Sl-lncRNA39896-knockout mutants than in wild-type plants”
e2“the mutants displayed enhanced resistance to P. infestans”
Claim · model-written summary
Overexpressing Sl-miR166b gave a phenotype similar to that of Sl-lncRNA39896-knockout.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Overexpressing Sl-miR166be3
- Gene
- Sl-miR166be3
- Outcome
- phenotypee3
- Direction
- no_changeinferrede3
- Comparator
- Sl-lncRNA39896-knockoute3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e3“Overexpressing Sl-miR166b yielded a similar phenotype to that produced by Sl-lncRNA39896-knockout”
Claim · model-written summary
Silencing of Sl-miR166b impaired tomato resistance to P. infestans.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencing of Sl-miR166be4
- Gene
- Sl-miR166be4
- Outcome
- resistancee4
- Direction
- impairede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e4“silencing of Sl-miR166b impaired resistance”
Claim · model-written summary
Sl-miR166b cleaved transcripts of its target class III homeodomain-leucine zipper genes SlHDZ34 and SlHDZ45.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlHDZ34 and SlHDZ45e5
- Outcome
- cleaved transcriptse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e5“We verified that Sl-miR166b cleaved transcripts of its target class III homeodomain-leucine zipper genes SlHDZ34 and SlHDZ45”
Claim · model-written summary
Silencing of SlHDZ34/45 decreased P. infestans accumulation in infected tomato plants.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Silencing of SlHDZ34/45e6
- Interactor
- P. infestansPhytophthora infestanse6pathogen
- Gene
- SlHDZ34/45e6
- Outcome
- pathogen accumulatione6
- Direction
- decreasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e6“Silencing of SlHDZ34/45 decreased pathogen accumulation in plants infected with P. infestans”
Claim · model-written summary
Jasmonic acid and ethylene contents were elevated in tomato plants following infection, in plants with enhanced resistance.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- infectione7
- Outcome
- jasmonic acid and ethylene contentse7
- Direction
- elevatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e7“Additionally, jasmonic acid and ethylene contents were elevated following infection in the plants with enhanced resistance”
Claim · model-written summary
Sl-lncRNA39896 is the first known lncRNA to negatively regulate tomato resistance to P. infestans.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- Sl-lncRNA39896e1
- Outcome
- resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e1“Sl-lncRNA39896 is the first known lncRNA to negatively regulate resistance to P. infestans in tomato”
Claim · model-written summary
The authors propose that the lncRNA39896-miR166b-HDZ module modulates tomato resistance to P. infestans.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- lncRNA39896-miR166b-HDZ modulee1
- Outcome
- resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e1“We propose a novel mechanism in which the lncRNA39896-miR166b-HDZ module modulates resistance to P. infestans”
Claim · model-written summary
A six-point mutation in the Sl-lncRNA39896 region that binds Sl-miR166b disabled the interaction.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- six-point mutatione1
- Gene
- Sl-lncRNA39896e1
- Outcome
- the interactione1
- Direction
- disablede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e1“A six-point mutation in the region of Sl-lncRNA39896 that binds to Sl-miR166b disabled the interaction”
Claim · model-written summary
Sl-lncRNA39896 acts as an endogenous target mimic (eTM) for Sl-miR166b.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- Sl-lncRNA39896e1
- Outcome
- acts as an eTM for Sl-miR166be1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield and quality of tomatoes (Solanum lycopersicum) is seriously affected by Phytophthora infestans”
e1“suggesting that Sl-lncRNA39896 acts as an eTM for Sl-miR166b”
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