Abstract
Alternative splicing is an important mechanism involved in regulating the development of multicellular organisms. Although many genes in plants undergo alternative splicing, little is understood of its significance in regulating plant growth and development. In this study, alternative splicing of black cottonwood (Populus trichocarpa) wood-associated NAC domain transcription factor (PtrWNDs), PtrWND1B, is shown to occur exclusively in secondary xylem fiber cells. PtrWND1B is expressed with a normal short-transcript PtrWND1B-s as well as its alternative long-transcript PtrWND1B-l. The intron 2 structure of the PtrWND1B gene was identified as a critical sequence that causes PtrWND1B alternative splicing. Suppression of PtrWND1B expression specifically inhibited fiber cell wall thickening. The two PtrWND1B isoforms play antagonistic roles in regulating cell wall thickening during fiber cell differentiation in Populus spp. PtrWND1B-s overexpression enhanced fiber cell wall thickening, while overexpression of PtrWND1B-l repressed fiber cell wall thickening. Alternative splicing may enable more specific regulation of processes such as fiber cell wall thickening during wood formation.
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Claims 6
Claim · model-written summary
PtrWND1B alternative splicing occurred exclusively in secondary xylem fiber cells.
- Taxon
- black cottonwood
Populus trichocarpae0 - Organ / stage
- secondary xylem fiber cellse0
- Gene
- PtrWND1Be0
- Outcome
- alternative splicinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alternative splicing of black cottonwood (Populus trichocarpa) wood-associated NAC domain transcription factor (PtrWNDs), PtrWND1B, is shown to occur exclusively in secondary xylem fiber cells”
Claim · model-written summary
PtrWND1B was expressed as short and long transcripts.
- Gene
- PtrWND1Be0
- Outcome
- normal short-transcript PtrWND1B-s as well as its alternative long-transcript PtrWND1B-le0
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrWND1B is expressed with a normal short-transcript PtrWND1B-s as well as its alternative long-transcript PtrWND1B-l”
t0“Populus Species”
Claim · model-written summary
The intron 2 structure caused PtrWND1B alternative splicing.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The intron 2 structure of the PtrWND1B gene was identified as a critical sequence that causes PtrWND1B alternative splicing”
t0“Populus Species”
Claim · model-written summary
Suppressing PtrWND1B inhibited fiber cell wall thickening.
- Treatment
- Suppression of PtrWND1B expressione0
- Gene
- PtrWND1Be0
- Outcome
- fiber cell wall thickeninge0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of PtrWND1B expression specifically inhibited fiber cell wall thickening”
t0“Populus Species”
Claim · model-written summary
PtrWND1B-s overexpression enhanced fiber cell wall thickening.
- Treatment
- PtrWND1B-s overexpressione0
- Gene
- PtrWND1B-se0
- Outcome
- fiber cell wall thickeninge0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrWND1B-s overexpression enhanced fiber cell wall thickening”
t0“Populus Species”
Claim · model-written summary
PtrWND1B-l overexpression repressed fiber cell wall thickening.
- Treatment
- overexpression of PtrWND1B-le0
- Gene
- PtrWND1B-le0
- Outcome
- fiber cell wall thickeninge0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PtrWND1B-l repressed fiber cell wall thickening”
t0“Populus Species”
The same questions in other papers
loss of function · cytology · Populus (poplar)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.