Abstract
SUMMARY High‐affinity K + transporters/K + uptake permeases/K + transporters (HAK/KUP/KT) are important pathways mediating K + transport across cell membranes, which function in maintaining K + homeostasis during plant growth and stress response. An increasing number of studies have shown that HAK/KUP/KT transporters play crucial roles in root K + uptake and root‐to‐shoot translocation. However, whether HAK/KUP/KT transporters also function in phloem K + translocation remain unclear. In this study, we revealed that a phloem‐localized rice HAK/KUP/KT transporter, OsHAK18, mediated cell K + uptake when expressed in yeast, Escherichia coli and Arabidopsis. It was localized at the plasma membrane. Disruption of OsHAK18 rendered rice seedlings insensitive to low‐K + (LK) stress. After LK stress, some WT leaves showed severe wilting and chlorosis, whereas the corresponding leaves of oshak18 mutant lines (a Tos17 insertion line and two CRISPR lines) remained green and unwilted. Compared with WT, the oshak18 mutants accumulated more K + in shoots but less K + in roots after LK stress, leading to a higher shoot/root ratio of K + per plant. Disruption of OsHAK18 does not affect root K + uptake and K + level in xylem sap, but it significantly decreases phloem K + concentration and inhibits root‐to‐shoot‐to‐root K + (Rb + ) translocation in split‐root assay. These results reveal that OsHAK18 mediates phloem K + loading and redistribution, whose disruption is in favor of shoot K + retention under LK stress. Our findings expand the understanding of HAK/KUP/KT transporters' functions and provide a promising strategy for improving rice tolerance to K + deficiency.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
OsHAK18 mediated cellular K + uptake when expressed in yeast, Escherichia coli and Arabidopsis.
- Taxon
- rice
Oryza sativae0 - Gene
- OsHAK18e0
- Outcome
- cell K + uptakee0
- Qualifier
- when expressed in yeast, Escherichia coli and Arabidopsise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a phloem‐localized rice HAK/KUP/KT transporter, OsHAK18, mediated cell K + uptake when expressed in yeast, Escherichia coli and Arabidopsis”
Claim · model-written summary
OsHAK18 localized to the plasma membrane.
- Taxon
- RiceOryza sativat0
- Gene
- OsHAK18t0
- Outcome
- localized at the plasma membranee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was localized at the plasma membrane”
t0“Rice potassium transporter OsHAK18”
Claim · model-written summary
Disrupting OsHAK18 made rice seedlings insensitive to low‐K + stress.
- Taxon
- riceOryza sativae0
- Treatment
- Disruption of OsHAK18e0
- Gene
- OsHAK18e0
- Outcome
- rice seedlings insensitive to low‐K + (LK) stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of OsHAK18 rendered rice seedlings insensitive to low‐K + (LK) stress”
Claim · model-written summary
After low-K stress, oshak18 mutant leaves remained green and unwilted while corresponding WT leaves wilted and became chlorotic.
- Taxon
- RiceOryza sativat0
- Material
- oshak18 mutant linese0
- Organ / stage
- leavese0
- Treatment
- LK stresse0
- Outcome
- remained green and unwiltede0
- Comparator
- WT leavese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After LK stress, some WT leaves showed severe wilting and chlorosis, whereas the corresponding leaves of oshak18 mutant lines (a Tos17 insertion line and two CRISPR lines) remained green and unwilted”
t0“Rice potassium transporter”
Claim · model-written summary
Under low-K stress, oshak18 mutants accumulated more shoot K + than WT.
- Taxon
- RiceOryza sativat0
- Material
- oshak18 mutantse0
- Organ / stage
- shootse0
- Treatment
- LK stresse0
- Outcome
- K + in shootse0
- Direction
- moree0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with WT, the oshak18 mutants accumulated more K + in shoots but less K + in roots after LK stress”
t0“Rice potassium transporter”
Claim · model-written summary
Under low-K stress, oshak18 mutants accumulated less root K + than WT.
- Taxon
- RiceOryza sativat0
- Material
- oshak18 mutantse0
- Organ / stage
- rootse0
- Treatment
- LK stresse0
- Outcome
- K + in rootse0
- Direction
- lesse0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with WT, the oshak18 mutants accumulated more K + in shoots but less K + in roots after LK stress”
t0“Rice potassium transporter”
Claim · model-written summary
The mutants had a higher shoot to root K + ratio per plant.
- Taxon
- RiceOryza sativat0
- Outcome
- shoot/root ratio of K + per plante0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leading to a higher shoot/root ratio of K + per plant”
t0“Rice potassium transporter”
Claim · model-written summary
Disrupting OsHAK18 did not affect root K + uptake.
- Taxon
- RiceOryza sativat0
- Treatment
- Disruption of OsHAK18e0
- Outcome
- root K + uptakee0
- Direction
- does not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of OsHAK18 does not affect root K + uptake and K + level in xylem sap”
t0“Rice potassium transporter”
Claim · model-written summary
Disrupting OsHAK18 did not affect K + level in xylem sap.
- Taxon
- RiceOryza sativat0
- Treatment
- Disruption of OsHAK18e0
- Outcome
- K + level in xylem sape0
- Direction
- does not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of OsHAK18 does not affect root K + uptake and K + level in xylem sap”
t0“Rice potassium transporter”
Claim · model-written summary
Disrupting OsHAK18 significantly decreased phloem K + concentration.
- Taxon
- RiceOryza sativat0
- Treatment
- Disruption of OsHAK18e1
- Outcome
- phloem K + concentratione0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it significantly decreases phloem K + concentration”
e1“Disruption of OsHAK18 does not affect root K + uptake”
t0“Rice potassium transporter”
Claim · model-written summary
Disrupting OsHAK18 inhibited root‐to‐shoot‐to‐root K + (Rb + ) translocation in a split-root assay.
- Taxon
- RiceOryza sativat0
- Treatment
- Disruption of OsHAK18e1
- Outcome
- root‐to‐shoot‐to‐root K + (Rb + ) translocatione0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibits root‐to‐shoot‐to‐root K + (Rb + ) translocation in split‐root assay”
e1“Disruption of OsHAK18 does not affect root K + uptake”
t0“Rice potassium transporter”
Claim · model-written summary
The authors conclude that OsHAK18 mediates phloem K + loading and redistribution.
- Taxon
- RiceOryza sativat0
- Gene
- OsHAK18e0
- Outcome
- phloem K + loading and redistributione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHAK18 mediates phloem K + loading and redistribution”
t0“Rice potassium transporter”
Claim · model-written summary
The authors identify OsHAK18 disruption as a promising strategy for improving rice tolerance to potassium deficiency.
- Taxon
- riceOryza sativae0
- Treatment
- disruptione1
- Gene
- OsHAK18t0
- Outcome
- improving rice tolerance to K + deficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“provide a promising strategy for improving rice tolerance to K + deficiency”
e1“whose disruption is in favor of shoot K + retention under LK stress”
t0“Rice potassium transporter OsHAK18”
The same questions in other papers
loss of function · organism · Oryza sativa (rice)Positive and negative results
6 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- no effect
8 papers more in this group
See all 12 papers →loss of function · nutrient: content · Oryza sativa (rice)Positive and negative results
35 papers in the same direction as this paper · 20 in the opposite direction
- positive
- positive
- positive
52 papers more in this group
See all 56 papers →loss of function · molecular: metabolites · Oryza sativa (rice)Positive and negative results
52 papers in the same direction as this paper · 18 in the opposite direction
- negative
- positive
- positive
67 papers more in this group
See all 71 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.