Abstract
Drought is a major environmental stress limiting global wheat (Triticum aestivum) production. Exploring drought tolerance genes is important for improving drought adaptation in this crop. Here, we cloned and characterized TaTIP41, a novel drought tolerance gene in wheat. TaTIP41 is a putative conserved component of target of rapamycin (TOR) signaling, and the TaTIP41 homoeologs were expressed in response to drought stress and abscisic acid (ABA). The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect. Furthermore, TaTIP41 physically interacted with TaTAP46, another conserved component of TOR signaling. Like TaTIP41, TaTAP46 positively regulated drought tolerance. Furthermore, TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase (PP2A) catalytic subunits, such as TaPP2A-2, and inhibited their enzymatic activities. Silencing TaPP2A-2 improved drought tolerance in wheat. Together, our findings provide new insights into the roles of TaTIP41 and TaTAP46 in the drought tolerance and ABA response in wheat, and their potential application in improving wheat environmental adaptability.
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Claims 10
Claim · model-written summary
TaTIP41 homoeologs are expressed in wheat in response to drought stress and abscisic acid.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- drought stress and abscisic acid (ABA)e1
- Gene
- TaTIP41e1
- Outcome
- TaTIP41 homoeologse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e1“the TaTIP41 homoeologs were expressed in response to drought stress and abscisic acid (ABA)”
Claim · model-written summary
Overexpression of TaTIP41 enhanced drought tolerance in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaTIP41e2
- Gene
- TaTIP41e2
- Outcome
- drought tolerancee2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e2“The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect”
Claim · model-written summary
Overexpression of TaTIP41 enhanced the ABA response in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaTIP41e2
- Gene
- TaTIP41e2
- Outcome
- ABA responsee2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e2“The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect”
Claim · model-written summary
Overexpression of TaTIP41 enhanced ABA-induced stomatal closure in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaTIP41e2
- Gene
- TaTIP41e2
- Outcome
- ABA-induced stomatal closuree2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e2“The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect”
Claim · model-written summary
RNAi downregulation of TaTIP41 decreased drought tolerance and the ABA response in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- downregulation using RNA interference (RNAi)e2
- Gene
- TaTIP41e2
- Outcome
- drought tolerancee2
- Direction
- decreaseinferrede2
- Qualifier
- ABA responsee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e2“The overexpression of TaTIP41 enhanced drought tolerance and the ABA response, including ABA-induced stomatal closure, while its downregulation using RNA interference (RNAi) had the opposite effect”
Claim · model-written summary
TaTAP46 positively regulates drought tolerance in wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaTAP46e3
- Outcome
- drought tolerancee3
- Direction
- positively regulatede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e3“Like TaTIP41, TaTAP46 positively regulated drought tolerance”
Claim · model-written summary
TaTIP41 physically interacts with TaTAP46 in wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaTIP41e4
- Outcome
- physically interacted with TaTAP46e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e4“TaTIP41 physically interacted with TaTAP46”
Claim · model-written summary
TaTIP41 and TaTAP46 interact with PP2A catalytic subunits such as TaPP2A-2 in wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaPP2A-2e5
- Outcome
- interacted with type-2A protein phosphatase (PP2A) catalytic subunitse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e5“TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase (PP2A) catalytic subunits, such as TaPP2A-2, and inhibited their enzymatic activities”
Claim · model-written summary
TaTIP41 and TaTAP46 inhibit the enzymatic activity of TaPP2A-2 in wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaPP2A-2e5
- Outcome
- enzymatic activitiese5
- Direction
- inhibitede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e5“TaTIP41 and TaTAP46 interacted with type-2A protein phosphatase (PP2A) catalytic subunits, such as TaPP2A-2, and inhibited their enzymatic activities”
Claim · model-written summary
Silencing TaPP2A-2 improved drought tolerance in wheat.
- Taxon
- wheatTriticum aestivume6
- Treatment
- Silencing TaPP2A-2e6
- Gene
- TaPP2A-2e6
- Outcome
- drought tolerancee6
- Direction
- improvede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“positively regulate drought tolerance in wheat”
e6“Silencing TaPP2A-2 improved drought tolerance in wheat”
The same questions in other papers
loss of function · tolerance: abiotic · Triticum aestivum (wheat)Positive and negative results
7 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.