Abstract
An in vitro mass propagation protocol was developed for Gerbera (Gerbera jamesonii Bolus) var. orange using flower bud explants. The young (7 days) explants were found to be suitable for culture and highest (100%) callus induction was obtained in MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAA. The maximum shoot regeneration (95%) and the number of shoots (10) were observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA. The highest shoot multiplication frequency (95%) and the number of shoots (60) were obtained in MS medium supplemented with only 2.0 mg/l BAP. The well-developed highest number of roots (5.0) per plant and average root length (5.0 cm) with 100% root induction efficiency without callusing was recorded in the half strength of MS medium devoid of any hormonal supplement. Acclimatized in vitro grown plantlets were successfully transferred into the field condition. Plant Tissue Cult. & Biotech. 32(2): 217-226, 2022 (December)
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Claims 10
Claim · model-written summary
Young (7 days) flower bud explants of Gerbera were suitable for culture.
GerberaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“The young (7 days) explants were found to be suitable for culture”
Claim · model-written summary
Highest callus induction (100%) in Gerbera was obtained in MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAA.
- Treatment
- MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAAe1
- Outcome
- callus inductione1
- Direction
- higheste1
- Value
- 100%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“highest (100%) callus induction was obtained in MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAA”
Claim · model-written summary
Maximum shoot regeneration (95%) in Gerbera was observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA.
- Treatment
- MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAAe1
- Outcome
- shoot regeneratione1
- Direction
- maximume1
- Value
- 95%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“The maximum shoot regeneration (95%) and the number of shoots (10) were observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA”
Claim · model-written summary
The maximum number of shoots (10) in Gerbera was observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA.
- Treatment
- MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAAe1
- Outcome
- number of shootse1
- Direction
- maximume1
- Value
- 10e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“The maximum shoot regeneration (95%) and the number of shoots (10) were observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA”
Claim · model-written summary
The highest shoot multiplication frequency (95%) in Gerbera was obtained in MS medium supplemented with only 2.0 mg/l BAP.
- Treatment
- MS medium supplemented with only 2.0 mg/l BAPe1
- Outcome
- shoot multiplication frequencye1
- Direction
- higheste1
- Value
- 95%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“The highest shoot multiplication frequency (95%) and the number of shoots (60) were obtained in MS medium supplemented with only 2.0 mg/l BAP”
Claim · model-written summary
The highest number of shoots (60) in Gerbera was obtained in MS medium supplemented with only 2.0 mg/l BAP.
- Treatment
- MS medium supplemented with only 2.0 mg/l BAPe1
- Outcome
- number of shootse1
- Direction
- higheste1
- Value
- 60e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“The highest shoot multiplication frequency (95%) and the number of shoots (60) were obtained in MS medium supplemented with only 2.0 mg/l BAP”
Claim · model-written summary
The highest number of roots per plant (5.0) in Gerbera was recorded in half-strength MS medium without hormonal supplement.
- Treatment
- half strength of MS medium devoid of any hormonal supplemente1
- Outcome
- number of rootse1
- Direction
- higheste1
- Value
- 5.0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“The well-developed highest number of roots (5.0) per plant and average root length (5.0 cm) with 100% root induction efficiency without callusing was recorded in the half strength of MS medium devoid of any hormonal supplement”
Claim · model-written summary
The highest average root length (5.0 cm) in Gerbera was recorded in half-strength MS medium without hormonal supplement.
- Treatment
- half strength of MS medium devoid of any hormonal supplemente1
- Outcome
- average root lengthe1
- Direction
- higheste1
- Value
- 5.0 cme1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“The well-developed highest number of roots (5.0) per plant and average root length (5.0 cm) with 100% root induction efficiency without callusing was recorded in the half strength of MS medium devoid of any hormonal supplement”
Claim · model-written summary
Half-strength MS medium without hormonal supplement gave 100% root induction efficiency without callusing in Gerbera.
- Treatment
- half strength of MS medium devoid of any hormonal supplemente1
- Outcome
- root induction efficiencye1
- Direction
- higheste1
- Value
- 100%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“The well-developed highest number of roots (5.0) per plant and average root length (5.0 cm) with 100% root induction efficiency without callusing was recorded in the half strength of MS medium devoid of any hormonal supplement”
Claim · model-written summary
Acclimatized in vitro grown Gerbera plantlets were successfully transferred into the field condition.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gerbera (Gerbera jamesonii Bolus)”
e1“Acclimatized in vitro grown plantlets were successfully transferred into the field condition”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.