Abstract
The rale of multiplication of gerbera (Gerbera jamesonii Bolus ex Hooker f.) through division is slow and season dependent. Somatic embryogenesis constitutes a powerful tool not only for rapid clonal propagation but for other biotechnological applications as well. The present investigation was carried out to study the response of explants to callus induction and somatic embryogenesis. Somatic embryogenesis has been achieved from callus cultures derived from leaf disc explants of gerbera cv. Alsmeera. Callus cultures were obtained from leaf disc pieces and petioles (1–2 cm) on MS medium supplemented with varied combinations and concentrations of auxins and cytokinins. High frequency (92.78%) for callus induction was achieved from leaf explant obtained on MS medium supplemented with BAP (1 mg/1) and 2, 4-D (2.5 mg/l). Maximum percentage response for somatic embryos induction (64.56%) from callus clumps was obtained on MS medium amended with BAP (0.5 mg/1) and NAA (1 mg/1). Histological studies confirmed the presence of somatic embryos in different developmental stages viz., globular and heart shaped.
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Claims 4
Claim · model-written summary
Somatic embryos were produced from leaf disc derived callus of gerbera cultivar Alsmeera.
GerberaNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryogenesis has been achieved from callus cultures derived from leaf disc explants of gerbera cv. Alsmeera.”
Claim · model-written summary
Leaf explants on MS medium with BAP and 2, 4-D reached 92.78% callus induction.
- Organ / stage
- leaf explante0
- Treatment
- MS medium supplemented with BAP (1 mg/1) and 2, 4-D (2.5 mg/l)e0
- Outcome
- callus inductione0
- Direction
- High frequencye0
- Value
- 92.78%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High frequency (92.78%) for callus induction was achieved from leaf explant obtained on MS medium supplemented with BAP (1 mg/1) and 2, 4-D (2.5 mg/l).”
t0“Gerbera (Gerbera Jamesonii Bolus ex Hooker f.)”
Claim · model-written summary
Callus clumps on BAP and NAA medium reached 64.56% somatic embryo induction.
- Organ / stage
- callus clumpse0
- Treatment
- MS medium amended with BAP (0.5 mg/1) and NAA (1 mg/1)e0
- Outcome
- somatic embryos inductione0
- Direction
- Maximume0
- Value
- 64.56%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum percentage response for somatic embryos induction (64.56%) from callus clumps was obtained on MS medium amended with BAP (0.5 mg/1) and NAA (1 mg/1).”
t0“Gerbera (Gerbera Jamesonii Bolus ex Hooker f.)”
Claim · model-written summary
Histology confirmed globular and heart shaped somatic embryos.
- Outcome
- presence of somatic embryos in different developmental stages viz., globular and heart shapede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histological studies confirmed the presence of somatic embryos in different developmental stages viz., globular and heart shaped.”
t0“Gerbera (Gerbera Jamesonii Bolus ex Hooker f.)”
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