Abstract
An experiment was undertaken to develop an improved in vitro regeneration protocol in gerbera. Murashige and Skoog (MS) medium was supplemented with various growth regulators at different concentrations for callus induction and organogenesis. Newly emerging leaves of Gerbera cv. Rosalin were used as explants. Experimental results showed that maximum rate (74.07%) of formation of callus with good growth was recorded on MS medium supplemented with 2.0mgL-1 2,4-D + BAP 0.5mgL-1. Best shoot regeneration (57.8 %) with maximum shoot number (12.0) was achieved on with BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS medium. Maximum (66.7 %) and earliest (12.3 days) root formation in shoots was recorded on IBA 3.0mgL-1is 1/2MS media. Survival rate of regenerated plantlets was maximum (73.33 %) in the potting mixture containing garden soil, sand and vermicompost (1:1:1).
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Claims 6
Claim · model-written summary
MS medium with 2.0mgL-1 2,4-D and BAP 0.5mgL-1 gave maximum callus formation of 74.07%.
- Material
- Rosaline1
- Treatment
- MS medium supplemented with 2.0mgL-1 2,4-D + BAP 0.5mgL-1e0
- Outcome
- formation of calluse0
- Direction
- maximume0
- Value
- 74.07%e0
GerberaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum rate (74.07%) of formation of callus with good growth was recorded on MS medium supplemented with 2.0mgL-1 2,4-D + BAP 0.5mgL-1”
e1“Gerbera cv. Rosalin”
Claim · model-written summary
BAP 2.0mgL-1 plus NAA 0.5mgL-1 gave best shoot regeneration of 57.8%.
- Material
- Rosaline1
- Treatment
- BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS mediume0
- Outcome
- shoot regeneratione0
- Direction
- Beste0
- Value
- 57.8 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Best shoot regeneration (57.8 %) with maximum shoot number (12.0) was achieved on with BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS medium.”
e1“Gerbera cv. Rosalin”
Claim · model-written summary
The same BAP and NAA medium gave the maximum shoot number of 12.0.
- Treatment
- BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS mediume0
- Outcome
- shoot numbere0
- Direction
- maximume0
- Value
- 12.0e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Best shoot regeneration (57.8 %) with maximum shoot number (12.0) was achieved on with BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS medium.”
e1“Gerbera cv. Rosalin”
Claim · model-written summary
IBA 3.0mgL-1 in half-strength MS medium gave maximum root formation of 66.7%.
- Treatment
- IBA 3.0mgL-1is 1/2MS mediae0
- Outcome
- root formation in shootse0
- Direction
- Maximume0
- Value
- 66.7 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum (66.7 %) and earliest (12.3 days) root formation in shoots was recorded on IBA 3.0mgL-1is 1/2MS media.”
e1“Gerbera cv. Rosalin”
Claim · model-written summary
IBA 3.0mgL-1 in half-strength MS medium gave earliest root formation at 12.3 days.
- Treatment
- IBA 3.0mgL-1is 1/2MS mediae0
- Outcome
- root formation in shootse0
- Direction
- earlieste0
- Value
- 12.3 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum (66.7 %) and earliest (12.3 days) root formation in shoots was recorded on IBA 3.0mgL-1is 1/2MS media.”
e1“Gerbera cv. Rosalin”
Claim · model-written summary
A 1:1:1 garden soil, sand, and vermicompost mix gave maximum plantlet survival of 73.33%.
- Treatment
- potting mixture containing garden soil, sand and vermicompost (1:1:1)e0
- Outcome
- Survival rate of regenerated plantletse0
- Direction
- maximume0
- Value
- 73.33 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Survival rate of regenerated plantlets was maximum (73.33 %) in the potting mixture containing garden soil, sand and vermicompost (1:1:1).”
e1“Gerbera cv. Rosalin”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.