Abstract
The influence of the developmental stage of zygotic embryos and the composition and pH of the Gamborg induction medium B5 on the initiation and development of somatic embryos was investigated. The optimal medium was B5 medium with a pH value of 5.0 and without plant growth regulator, at which the highest frequency of somatic embryogenesis (56.67%) and the highest average number of somatic embryos per explant (3.35) were achieved. Somatic embryos appeared directly on the hypocotyls of the explants, without the callus stage. On zygotic embryos in the early cotyledonary phase, three times higher regeneration was achieved compared to larger embryos in the cotyledonary phase. The induction of somatic embryogenesis did not occur during the growth of explants on the medium containinig 2,4-dichlorophenoxyacetic acid, nor on zygotic embryos in the late cotyledonary phase. Random amplified polymorphic DNA analysis showed the genetic stability of somatic embryo-derived plants, which makes this newly established protocol suitable for the regeneration and propagation of desirable broccoli genotypes.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
B5 medium at pH 5.0 without growth regulator achieved the highest somatic embryogenesis frequency, 56.67%.
- Taxon
- broccoli
Brassica oleracea var. italicat0 - Treatment
- B5 medium with a pH value of 5.0 and without plant growth regulatore0
- Outcome
- frequency of somatic embryogenesise0
- Direction
- higheste0
- Value
- 56.67%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal medium was B5 medium with a pH value of 5.0 and without plant growth regulator, at which the highest frequency of somatic embryogenesis (56.67%) and the highest average number of somatic embryos per explant (3.35) were achieved”
t0“broccoli”
Claim · model-written summary
B5 medium at pH 5.0 without growth regulator achieved the highest mean number of somatic embryos per explant, 3.35.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- B5 medium with a pH value of 5.0 and without plant growth regulatore0
- Outcome
- average number of somatic embryos per explante0
- Direction
- higheste0
- Value
- 3.35e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal medium was B5 medium with a pH value of 5.0 and without plant growth regulator, at which the highest frequency of somatic embryogenesis (56.67%) and the highest average number of somatic embryos per explant (3.35) were achieved”
t0“broccoli”
Claim · model-written summary
Somatic embryos formed directly on explant hypocotyls without callus.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Organ / stage
- hypocotylse0
- Outcome
- Somatic embryos appeared directlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos appeared directly on the hypocotyls of the explants, without the callus stage”
t0“broccoli”
Claim · model-written summary
Early cotyledonary embryos regenerated three times more than larger cotyledonary embryos.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Organ / stage
- early cotyledonary phasee0
- Outcome
- regeneratione0
- Direction
- highere0
- Value
- three timese0
- Comparator
- larger embryos in the cotyledonary phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On zygotic embryos in the early cotyledonary phase, three times higher regeneration was achieved compared to larger embryos in the cotyledonary phase”
t0“broccoli”
Claim · model-written summary
Somatic embryogenesis did not occur with 2,4-dichlorophenoxyacetic acid.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- medium containinig 2,4-dichlorophenoxyacetic acide0
- Outcome
- somatic embryogenesis did not occure0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of somatic embryogenesis did not occur during the growth of explants on the medium containinig 2,4-dichlorophenoxyacetic acid”
t0“broccoli”
Claim · model-written summary
Somatic embryogenesis did not occur in late cotyledonary zygotic embryos.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Organ / stage
- late cotyledonary phasee0
- Outcome
- somatic embryogenesis did not occure1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nor on zygotic embryos in the late cotyledonary phase”
e1“The induction of somatic embryogenesis did not occur”
t0“broccoli”
Claim · model-written summary
RAPD analysis showed genetic stability of somatic embryo derived broccoli plants.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Material
- somatic embryo-derived plantse0
- Outcome
- genetic stabilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Random amplified polymorphic DNA analysis showed the genetic stability of somatic embryo-derived plants”
t0“broccoli”
Claim · model-written summary
Authors consider the protocol suitable for regeneration and propagation of broccoli genotypes.
- Taxon
- broccoliBrassica oleracea var. italicae0
- Outcome
- regeneration and propagatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this newly established protocol suitable for the regeneration and propagation of desirable broccoli genotypes”
The same questions in other papers
2,4-D · growth · Brassica oleracea (wild cabbage)Positive and negative results
1 paper in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →ph · growth · Brassica oleracea (wild cabbage)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →class · growth · Brassica oleracea (wild cabbage)Positive and negative results
14 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
13 papers more in this group
See all 17 papers →in vitro · growth · Brassica oleracea (wild cabbage)Positive and negative results
34 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
34 papers more in this group
See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.