Abstract
Anthers from six cauliflower types (five Syrian local types, Gtyp1, Gtyp2, Gtyp3, Gtyp4, Gtyp5 and the control Casper F1), were cultured in vitro to observe their androgenic responses. Anthers were cultured on MS medium supplemented with diverse levels of 2,4-D and BAP. Among the local types Gtyp1 was significantly superior in the callus induction frequency (54.44%). While the maximum rate of callus induction (56.8%) was detected in MS medium (0.3 mg/L 2,4-D and 1 mg/L BAP). Then callus was cultured on MS medium supplemented with different levels of NAA, BA, BAP and 2iP to Shoot regeneration. The maximum rate of shoot initiation was noticed in Casper F1(39.31%). Also, shoot initiation was the best in MS medium (0.5 mg/L NAA and 2 mg/L 2iP). As well, the minimum number of days of initiated shoot regeneration was detected in Casper F1 (31.35 days). Ploidy analysis of root tips revealed that 11.43% of the regenerated plantlets were haploids, 55.71% were diploids, and 32.9% were polyploids.
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Claims 8
Claim · model-written summary
Gtyp1 had the highest local-type callus induction frequency, 54.44%.
- Taxon
- cauliflower
Brassica oleracea var. botrytist0 - Material
- Gtyp1e0
- Outcome
- callus induction frequencye0
- Direction
- superiore0
- Value
- 54.44%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the local types Gtyp1 was significantly superior in the callus induction frequency (54.44%)”
t0“cauliflower”
Claim · model-written summary
MS medium with 0.3 mg/L 2,4-D and 1 mg/L BAP produced 56.8% callus induction.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- MS medium (0.3 mg/L 2,4-D and 1 mg/L BAP)e0
- Outcome
- callus inductione0
- Direction
- maximume0
- Value
- 56.8%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum rate of callus induction (56.8%) was detected in MS medium (0.3 mg/L 2,4-D and 1 mg/L BAP)”
t0“cauliflower”
Claim · model-written summary
Casper F1 had the maximum shoot initiation rate of 39.31%.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Material
- Casper F1e0
- Outcome
- shoot initiatione0
- Direction
- maximume0
- Value
- 39.31%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum rate of shoot initiation was noticed in Casper F1(39.31%)”
t0“cauliflower”
Claim · model-written summary
Shoot initiation was best in MS medium with 0.5 mg/L NAA and 2 mg/L 2iP.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- MS medium (0.5 mg/L NAA and 2 mg/L 2iP)e0
- Outcome
- shoot initiatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot initiation was the best in MS medium (0.5 mg/L NAA and 2 mg/L 2iP)”
t0“cauliflower”
Claim · model-written summary
Casper F1 had the shortest time to shoot regeneration, 31.35 days.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Material
- Casper F1e0
- Outcome
- number of days of initiated shoot regeneratione0
- Direction
- minimume0
- Value
- 31.35 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“minimum number of days of initiated shoot regeneration was detected in Casper F1 (31.35 days)”
t0“cauliflower”
Claim · model-written summary
Haploids comprised 11.43% of regenerated plantlets.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- regenerated plantlets were haploidse0
- Value
- 11.43%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11.43% of the regenerated plantlets were haploids, 55.71% were diploids, and 32.9% were polyploids”
t0“cauliflower”
Claim · model-written summary
Diploids comprised 55.71% of regenerated plantlets.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- diploidse0
- Value
- 55.71%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11.43% of the regenerated plantlets were haploids, 55.71% were diploids, and 32.9% were polyploids”
t0“cauliflower”
Claim · model-written summary
Polyploids comprised 32.9% of regenerated plantlets.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- polyploidse0
- Value
- 32.9%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11.43% of the regenerated plantlets were haploids, 55.71% were diploids, and 32.9% were polyploids”
t0“cauliflower”
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