Abstract
Lead (Pb) is a highly toxic contaminant to living organisms and the environment. Excessive Pb in soils affects crop yield and quality, thus threatening human health via the food chain. Herein, we investigated Pb tolerance among a maize association panel using root bushiness (BSH) under Pb treatment as an indicator. Through a genome-wide association study of relative BSH, we identified four single nucleotide polymorphisms (SNPs) and 30 candidate genes associated with Pb tolerance in maize seedlings. Transcriptome analysis showed that four of the 30 genes were differentially responsive to Pb treatment between two maize lines with contrasting Pb tolerance. Among these, the ZmbZIP107 transcription factor was confirmed as the key gene controlling maize tolerance to Pb by using gene-based association studies. Two 5’ UTR_variants in ZmbZIP107 affected its expression level and Pb tolerance among different maize lines. ZmbZIP107 protein was specifically targeted to the nucleus and ZmbZIP107 mRNA showed the highest expression in maize seedling roots among different tissues. Heterologous expression of ZmbZIP107 enhanced rice tolerance to Pb stress and decreased Pb absorption in the roots. Our study provided the basis for revelation of the molecular mechanism underlying Pb tolerance and contributed to cultivation of Pb-tolerant varieties in maize.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
GWAS identified four SNPs associated with maize Pb tolerance.
Zea maysNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified four single nucleotide polymorphisms (SNPs) and 30 candidate genes associated with Pb tolerance in maize seedlings”
Claim · model-written summary
GWAS identified 30 candidate genes associated with maize Pb tolerance.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified four single nucleotide polymorphisms (SNPs) and 30 candidate genes associated with Pb tolerance in maize seedlings”
Claim · model-written summary
Four candidate genes responded differentially to Pb treatment in contrasting maize lines.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four of the 30 genes were differentially responsive to Pb treatment between two maize lines with contrasting Pb tolerance”
Claim · model-written summary
ZmbZIP107 was confirmed as a key gene controlling maize Pb tolerance.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmbZIP107 transcription factor was confirmed as the key gene controlling maize tolerance to Pb”
Claim · model-written summary
Two 5’ UTR variants in ZmbZIP107 affected expression level.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two 5’ UTR_variants in ZmbZIP107 affected its expression level and Pb tolerance among different maize lines.”
Claim · model-written summary
Two 5’ UTR variants in ZmbZIP107 affected Pb tolerance among maize lines.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two 5’ UTR_variants in ZmbZIP107 affected its expression level and Pb tolerance among different maize lines.”
Claim · model-written summary
ZmbZIP107 protein localized to the nucleus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmbZIP107 protein was specifically targeted to the nucleus”
e1“maize seedling roots among different tissues”
Claim · model-written summary
ZmbZIP107 mRNA expression was highest in maize seedling roots.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmbZIP107 mRNA showed the highest expression in maize seedling roots among different tissues.”
Claim · model-written summary
Heterologous ZmbZIP107 expression increased rice Pb tolerance.
- Taxon
- rice
Oryza sativae0 - Treatment
- Heterologous expression of ZmbZIP107e0
- Gene
- ZmbZIP107e0
- Outcome
- rice tolerance to Pb stresse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heterologous expression of ZmbZIP107 enhanced rice tolerance to Pb stress and decreased Pb absorption in the roots.”
Claim · model-written summary
Heterologous ZmbZIP107 expression decreased Pb absorption in rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Treatment
- Heterologous expression of ZmbZIP107e0
- Gene
- ZmbZIP107e0
- Outcome
- Pb absorptione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heterologous expression of ZmbZIP107 enhanced rice tolerance to Pb stress and decreased Pb absorption in the roots.”
The same questions in other papers
overexpression · nutrient: content · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →overexpression · disease · Oryza sativa (rice)Positive and negative results
23 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.