Paper · 2025 · Advances in Horticultural Science

Effect of auxins on the rooting of the avocado rootstock 'Duke 7'

Mercedes Martínez-Villagómez; Alejandro Facundo Barrientos-Priego; José Oscar Mascorro-Gallardo; et al.

9
claims
21
verbatim quotes
1
citations
3
research areas
✓
Open access · cc-by

Abstract

Clonal propagation of avocado rootstocks through etiolated shoot rooting represents a key strategy to enhance genetic uniformity, plant health, and productivity in commercial orchards. However, its success largely depends on the rooting phase, where auxins play a critical role. This study evaluated the effect of auxin-based rooting agents (types and concentrations) on root induction and quality in etiolated shoots of the ‘Duke 7’ rootstock. Five agents (IAA, NAA, IBA, K-IBA, and IBA + NAA combination) were tested at three concentrations (24.6, 34.4, and 44.2 mM) under a completely randomized factorial design (5 × 3) with three replicates per treatment. Morphological variables included rooting percentage, survival rate, root number/length/diameter, secondary root development, callus formation, and root quality index (RQI). Results revealed significant effects of agent type, concentration, and their interaction. NAA (34.4 mM) was the most effective for root number (55.3) and RQI (154.9 cm), albeit with high callus formation and reduced secondary roots. The IBA + NAA combination (34.4 mM) also showed high RQI (140.4 cm), with greater root length and less negative impact on root architecture. IBA alone achieved 100% rooting with moderate root development, balancing efficacy and physiological tolerance. Overall, intermediate concentrations of NAA and IBA + NAA yielded optimal results. These findings can refine clonal propagation protocols for ‘Duke 7’, with direct applications in commercial nurseries producing high-performance rootstocks.

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Claims 9

Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Rooting responses depended significantly on agent type, concentration, and their interaction.

Taxon
avocadoPersea americanat0
Outcome
effects of agent type, concentration, and their interactione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Results revealed significant effects of agent type, concentration, and their interaction.”

✓ located verbatim in the source · abstract, characters 834–923 · show in text ↑
t0

“avocado”

✓ located verbatim in the source · title, characters 39–46 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NAA at 34.4 mM produced the most roots, 55.3.

Taxon
avocadoPersea americanat0
Material
Duke 7t0
Treatment
NAA (34.4 mM)e0
Outcome
root numbere0
Direction
most effectivee0
Value
55.3e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“NAA (34.4 mM) was the most effective for root number (55.3) and RQI (154.9 cm)”

✓ located verbatim in the source · abstract, characters 924–1002 · show in text ↑
t0

“avocado rootstock 'Duke 7'”

✓ located verbatim in the source · title, characters 39–65 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NAA at 34.4 mM yielded an RQI of 154.9 cm.

Taxon
avocadoPersea americanat0
Treatment
NAA (34.4 mM)e0
Outcome
RQIe0
Direction
most effectivee0
Value
154.9 cme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“NAA (34.4 mM) was the most effective for root number (55.3) and RQI (154.9 cm)”

✓ located verbatim in the source · abstract, characters 924–1002 · show in text ↑
t0

“avocado”

✓ located verbatim in the source · title, characters 39–46 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

NAA at 34.4 mM caused high callus formation.

Taxon
avocadoPersea americanat0
Treatment
NAA (34.4 mM)e1
Outcome
callus formatione0
Direction
highe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“albeit with high callus formation and reduced secondary roots”

✓ located verbatim in the source · abstract, characters 1004–1065 · show in text ↑
t0

“avocado”

✓ located verbatim in the source · title, characters 39–46 · show in text ↑
e1

“NAA (34.4 mM) was the most effective for root number (55.3) and RQI (154.9 cm)”

✓ located verbatim in the source · abstract, characters 924–1002 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

NAA at 34.4 mM reduced secondary roots.

Taxon
avocadoPersea americanat0
Treatment
NAA (34.4 mM)e1
Outcome
secondary rootse0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“albeit with high callus formation and reduced secondary roots”

✓ located verbatim in the source · abstract, characters 1004–1065 · show in text ↑
t0

“avocado”

✓ located verbatim in the source · title, characters 39–46 · show in text ↑
e1

“NAA (34.4 mM) was the most effective for root number (55.3) and RQI (154.9 cm)”

✓ located verbatim in the source · abstract, characters 924–1002 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IBA plus NAA at 34.4 mM yielded an RQI of 140.4 cm.

Taxon
avocadoPersea americanat0
Treatment
IBA + NAA combination (34.4 mM)e0
Outcome
RQIe0
Direction
highe0
Value
140.4 cme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The IBA + NAA combination (34.4 mM) also showed high RQI (140.4 cm)”

✓ located verbatim in the source · abstract, characters 1067–1134 · show in text ↑
t0

“avocado”

✓ located verbatim in the source · title, characters 39–46 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IBA plus NAA produced greater root length and less negative impact on root architecture.

Taxon
avocadoPersea americanat0
Treatment
IBA + NAA combination (34.4 mM)e1
Outcome
root length and less negative impact on root architecturee0
Direction
greatere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“with greater root length and less negative impact on root architecture.”

✓ located verbatim in the source · abstract, characters 1136–1207 · show in text ↑
t0

“avocado”

✓ located verbatim in the source · title, characters 39–46 · show in text ↑
e1

“The IBA + NAA combination (34.4 mM) also showed high RQI (140.4 cm)”

✓ located verbatim in the source · abstract, characters 1067–1134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IBA alone achieved 100% rooting.

Taxon
avocadoPersea americanat0
Treatment
IBA alonee0
Outcome
rootinge0
Direction
achievede0
Value
100%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“IBA alone achieved 100% rooting with moderate root development”

✓ located verbatim in the source · abstract, characters 1208–1270 · show in text ↑
t0

“avocado”

✓ located verbatim in the source · title, characters 39–46 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

IBA alone produced moderate root development.

Taxon
avocadoPersea americanat0
Treatment
IBA alonee0
Outcome
root developmente0
Direction
moderatee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Persea americana
Persea americana · TaxonB.navez · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“IBA alone achieved 100% rooting with moderate root development”

✓ located verbatim in the source · abstract, characters 1208–1270 · show in text ↑
t0

“avocado”

✓ located verbatim in the source · title, characters 39–46 · show in text ↑

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.