Abstract
Shoot tip explants of superior date palm cultivars Sewi, Tamr El wady, Hegazi and one individual unknown female from El-Kharga city (called Faleg or Meghel) were grown in the New Valley region and cultured on three different starting culture media MS+10mg/l 2,4-D+3.0mg/l 2iP ( M1), MS+ 10mg/l NAA+ 3.0mg/l 2iP (M2) and MS+30 mg/l NAA+20mg/l 2iP(M3) for eight months (two months interval). All culture media were supplemented with 40g/l sucrose, 2.0 g/l PVP, and 3.0 g/l activated charcoal and solidified with gelrite at 2.0 g/l. After eight months, some morphological responses were noticed as direct shoot buds, direct embryogenesis and callus. Depending on these responses, different culture media with different combinations of auxin and cytokinin were used to differentiate and multiply these cultures. Results indicated that the highest direct shoot bud percentage was formed on M1 medium while the highest direct embryo percentage was formed on M2.Induced direct shoot buds that transferred to the half strength of 2,4D then transferred to auxin free medium showed the highest multiplied rate as clusters. Maximum shoot number was obtained when transferring these clusters to a modified medium supplemented with 3/4 MS salt + 2.0 mg/l BA+ 0.5 mg/l Kinetin+ 0.25 mg/l 2iP+ 1.0 mg/l IAA. Meanwhile, induced direct embryos transferred to a modified medium supplemented with 1/2 MS+ 0.25 mg/l ABA + 0.5 mg/l Kinetin+ 0.25 mg/l 2iP showed the highest secondary embryo number. All induced shoots were rooted successfully and transferred to the greenhouse.
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Claims 6
Claim · model-written summary
M1 medium gave the highest direct shoot bud percentage.
- Taxon
- date palm
Phoenix dactyliferat0 - Treatment
- M1 mediume0
- Outcome
- direct shoot bud percentagee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm”
e0“highest direct shoot bud percentage was formed on M1 medium”
Claim · model-written summary
M2 medium gave the highest direct embryo percentage.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- M2e0
- Outcome
- direct embryo percentagee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm”
e0“highest direct embryo percentage was formed on M2”
Claim · model-written summary
Transferring direct shoot buds to half-strength 2,4D and then auxin-free medium gave the highest cluster multiplication rate.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- half strength of 2,4D then transferred to auxin free mediume0
- Outcome
- multiplied rate as clusterse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm”
e0“Induced direct shoot buds that transferred to the half strength of 2,4D then transferred to auxin free medium showed the highest multiplied rate as clusters”
Claim · model-written summary
The modified medium with 3/4 MS salt and listed growth regulators produced the maximum shoot number.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- modified medium supplemented with 3/4 MS salt + 2.0 mg/l BA+ 0.5 mg/l Kinetin+ 0.25 mg/l 2iP+ 1.0 mg/l IAAe0
- Outcome
- shoot numbere0
- Direction
- Maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm”
e0“Maximum shoot number was obtained when transferring these clusters to a modified medium supplemented with 3/4 MS salt + 2.0 mg/l BA+ 0.5 mg/l Kinetin+ 0.25 mg/l 2iP+ 1.0 mg/l IAA”
Claim · model-written summary
The modified 1/2 MS medium with ABA, Kinetin, and 2iP gave the highest secondary embryo number.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- modified medium supplemented with 1/2 MS+ 0.25 mg/l ABA + 0.5 mg/l Kinetin+ 0.25 mg/l 2iPe0
- Outcome
- secondary embryo numbere0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm”
e0“induced direct embryos transferred to a modified medium supplemented with 1/2 MS+ 0.25 mg/l ABA + 0.5 mg/l Kinetin+ 0.25 mg/l 2iP showed the highest secondary embryo number”
Claim · model-written summary
All induced shoots rooted and were transferred to a greenhouse.
- Taxon
- date palmPhoenix dactyliferat0
- Organ / stage
- induced shootse0
- Outcome
- rooted successfully and transferred to the greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm”
e0“All induced shoots were rooted successfully and transferred to the greenhouse”
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