Abstract
In order to obtain a basic information of plant cell suspension culture as a step toward the development of large scale culture, culture conditions of crown gall cells (auxin non-requiring cells) were investigated. Addition of yeast extract to culture medium was significantly effective for the growth and cell dispersion. In experiments on the ability of the cultured cells to utilize sugars as the carbon source, it was observed that galactose, added to the culture medium, markedly inhibited the cell growth. Pasteurization of the medium containing fructose as carbon source made it brownish by Maillard reaction and the medium apparently restrained the cell growth. However, the fructose medium sterilized by filtration was excellent for the cell growth as well as sucrose or glucose medium. In a jar fermentor, even the glucose medium became brownish by heat sterilization and the brown colored medium restrained the cell growth. Under optimum conditions, the doubling time was 1.1 day in exponential phase and 2.0 g of cell (dry weight) per 100 ml culture was obtained as the maximum yield.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Yeast extract significantly improved cell growth and dispersion.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Treatment
- yeast extracte0
- Outcome
- growth and cell dispersione0
- Direction
- effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Addition of yeast extract to culture medium was significantly effective for the growth and cell dispersion.”
Claim · model-written summary
Galactose markedly inhibited cell growth.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- galactosee0
- Outcome
- cell growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“galactose, added to the culture medium, markedly inhibited the cell growth”
Claim · model-written summary
Heat-sterilized fructose medium browned and restrained cell growth.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Pasteurization of the medium containing fructosee0
- Outcome
- cell growthe0
- Direction
- restrainede0
- Qualifier
- apparentlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Pasteurization of the medium containing fructose as carbon source made it brownish by Maillard reaction and the medium apparently restrained the cell growth.”
Claim · model-written summary
Filter-sterilized fructose medium supported excellent cell growth, as did sucrose or glucose medium.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- fructose medium sterilized by filtratione0
- Outcome
- cell growthe0
- Direction
- excellente0
- Comparator
- sucrose or glucose mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“the fructose medium sterilized by filtration was excellent for the cell growth as well as sucrose or glucose medium”
Claim · model-written summary
Heat-sterilized glucose medium browned and restrained cell growth in a jar fermentor.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- heat sterilizatione0
- Outcome
- cell growthe0
- Direction
- restrainede0
- Setting
- jar fermentore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“In a jar fermentor, even the glucose medium became brownish by heat sterilization and the brown colored medium restrained the cell growth.”
Claim · model-written summary
Under optimum conditions doubling time was 1.1 day in exponential phase.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- doubling timee0
- Value
- 1.1 daye0
- Qualifier
- optimum conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Under optimum conditions, the doubling time was 1.1 day in exponential phase”
Claim · model-written summary
Maximum cell dry-weight yield was 2.0 g per 100 ml culture.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- maximum yielde0
- Value
- 2.0 g of cell (dry weight) per 100 ml culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“2.0 g of cell (dry weight) per 100 ml culture was obtained as the maximum yield”
The same questions in other papers
heat · growth · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 11 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →processing · growth · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 13 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →botanical · growth · Nicotiana tabacum (tobacco)Positive and negative results
14 papers in the same direction as this paper · 9 in the opposite direction
- negative
- no effect
- negative
20 papers more in this group
See all 24 papers →in vitro · growth · Nicotiana tabacum (tobacco)Positive and negative results
31 papers in the same direction as this paper · 8 in the opposite direction
- negative
- negative
- negative
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.